3D hepatoma tissue construction method based on biological 3D printing hydrogel

Abstract

The invention discloses a 3D hepatoma tissue construction method based on biological 3D printing hydrogel. The method comprises the steps as follows: 1) a GelMA biomaterial is synthesized, and the acylation rate and light polymerization time of the GelMA biomaterial are detected; 2) after GelMA and an photoinitiator are uniformly mixed and dissolved, human hepatoma cell HepG2 and GelMA solutions are uniformly mixed, the cell concentration is adjusted to 1*10<6>/mL, and biological printing ink is prepared. Biological printed cells and a hydrogel system are placed under an ultraviolet lamp for light polymerization for 5 min to be cured, the cured system is placed in a 10%FBS culture solution to be cultured for 7 d, and a hepatoma tissue engineered unit containing an HepG2 source is prepared and saved for later use; 3) the swelling ratio and balance water content of a 3D hepatoma tissue material are detected; 4) the growth states of 2D and 3D on the seventh day are detected with a BE histochemistry method. The method is reliable, convenient to operate and high in repeatability and can effectively promote formation of hepatoma tissue functions and provide beneficial reference for scientific research of hepatic tissue engineering and clinical treatment of hepatoma.

Description

technical field [0001] The invention belongs to the technical field of biology and tissue engineering, and in particular relates to a method for constructing three-dimensional liver cancer tissue based on biological 3D printing hydrogel. Background technique [0002] Drug development requires in vivo and in vitro experiments. The liver cancer cell line HepG2 has been extensively studied and applied in drug screening and tissue engineering in traditional 2D culture. Many indicators cannot be evaluated objectively, which is still a difficult problem to be overcome. The 3D culture system can better simulate the growth environment of cells in the human body, has a more complete culture system than 2D, and is being widely used in various fields. [0003] The 3D culture system needs to be realized with the help of different biological materials. Biological scaffold materials such as collagen and nanomaterials are widely used in tissue engineering. Cell growth requires certain me...

AI Technical Summary

Problems solved by technology

[0002] Drug development requires in vivo and in vitro experiments. The liver cancer cell line HepG2 has been extensively studied and applied in drug screening and tissue engineering in traditional 2D culture. Many indicators cannot be evaluated objectively, which is still a difficult problem to be overcome

Images