Resist calcification modified method of heterogeneity biological organization material

A biological tissue and tissue material technology, applied in medical science, prosthesis, etc., can solve the problems of calcium salt deposition, no cross-linking, easy tearing, etc., achieve high tensile strength and elongation at break, biomechanical Enhanced performance, enhanced biostability

Inactive Publication Date: 2008-04-16
胡盛寿 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, glutaraldehyde treatment also has its own shortcomings. First, it only has a certain cross-linking effect on collagen fibers, but has no cross-linking effect on other components such as elastic fibers and proteoglycans; Calcification
The calcified tissue will become hard and brittle, which will affect the activity of the tissue and be easily torn and perforated, resulting in poor durability of the biomaterial. The patient must undergo a second operation to remove the calcified tissue, which not only poses a certain risk but also increases the patient’s Un

Method used

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  • Resist calcification modified method of heterogeneity biological organization material
  • Resist calcification modified method of heterogeneity biological organization material
  • Resist calcification modified method of heterogeneity biological organization material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1 Using tannic acid alone to cross-link the bovine jugular vein with valve;

[0088] (1) Reagent preparation

[0089] 2.5% pH4.0 tannic acid solution: get 12.5 grams of commercially available tannic acid and dissolve it in 50mmol / LNa2HPO4 solution to 500ml, adjust the pH to 4.0 with hydrochloric acid.

[0090] (2) Material preparation

[0091] The bovine jugular vein pipeline with a diameter of 12-24 mm was collected and the length was about 10 cm. The fat and excess loose connective tissue on the surface were trimmed, and rinsed in D-Hank’s solution for later use.

[0092] (3) Cross-linking treatment process

[0093] 1. Rinse the inside and outside of the bovine jugular vein lumen with 2.5% pH4.0 tannin solution first, then fill the cavity with 2.5% pH4.0 tannin solution, and then insert 300ml of 2.5% pH4.0 Soak in a container of tannin solution for 48 hours at 32 °C.

[0094] 2. Before use, put the above-mentioned material treated by this method into norma...

Embodiment 2

[0114] Example 2 Synergistic crosslinking of tannic acid and glutaraldehyde to treat bovine jugular vein valved pipeline;

[0115] (1) Reagent preparation

[0116] 0.3% glutaraldehyde / 0.3% tannin mixed solution pH5.5: Dissolve 3ml of commercially available 50% glutaraldehyde solution in 50mmol / L Na 2 HPO 4 solution to 500ml, then take 0.5 g of commercially available tannic acid and dissolve it in the above solution, and adjust the pH to 5.5 with hydrochloric acid.

[0117] (2) Material preparation

[0118] Bovine jugular veins with a diameter of 12-24 mm and a length of about 10 cm were collected, the fat on the surface and excess loose connective tissue were trimmed, and rinsed in D-Hank’s solution for later use.

[0119] (3) Cross-linking treatment process

[0120] 1. Rinse the inside and outside of the cavity with 0.3% glutaraldehyde / 0.3% tannin mixed solution at pH5.5, then fill the cavity with 0.3% glutaraldehyde / 0.3% tannin mixed solution and put in 300ml Soak in a ...

Embodiment 3

[0141] Embodiment 3 Using tannic acid alone to cross-link the bovine pericardium;

[0142] (1) Reagent preparation

[0143] 1% pH5.5 tannic acid solution: prepare 50mmol / L Na 2 HPO 4 buffer solution to 500ml, then take 5 grams of commercially available tannic acid and dissolve it in the above solution, and adjust the pH value to 5.5 with hydrochloric acid.

[0144] (2) Material preparation

[0145] The collection area is about 8×8cm 2 Trim the fat and excess loose connective tissue from the bovine pericardium, rinse in D-Hank’s solution and set aside.

[0146] (3) Cross-linking treatment process

[0147] 1. Rinse the bovine pericardium material once with 1% pH5.5 tannic acid solution, then put it into a container containing 300ml of 1% pH5.5 tannic acid solution and fix it at 22°C for 96 hours.

[0148] 2. Before use, place the above-mentioned material treated by this method into normal saline and rinse it several times to remove residual tannin that has not been combine...

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Abstract

The present invention discloses a method of calcification-resisting modifying for the heterologous biology tissue material, in the method the tannic acid solution is used to immerse the tissue; the concentration of the tannic acid is between 1 and 6%, the pH value of the solution is between 4.0 and 6.0, the immersing temperature is between 18 and 32 DEG C, and the immersing time is between 24 and 96 hour. The method of the invention can simultaneously crosslink the collagen fiber, the elastic fiber and glycosaminoglycan in the cell epimatrix of the tissue material, reduce the degradation when the tissue is implanted to the body, compensate the insufficiency that the pentanedial only crosslinks the collagen fiber and remarkably increase the calcification-resisting capability of the material; has the effect of antibacterial and reducing the protein antigenicity, and helps to increase the calcification-resisting capability and bioavailability of the material. The intercrossed tissue material has the advantages of good biostability, reinforced biomechanical property and long service lifetime.

Description

technical field [0001] The invention relates to an anti-calcification modification method for biological tissue materials, in particular to an anti-calcification modification method for homogeneous or heterogeneous biological tissue materials. Background technique [0002] In cardiac surgery, in most cases, it is necessary to implant various repair materials to replace diseased tissues and organs and restore their functions. According to the source of implant materials, it can be roughly divided into two categories: artificial materials and biological materials, and biological materials usually need to be chemically modified to remove their immunogenicity, increase the ability to resist enzymatic hydrolysis, and improve the durability of materials in the body. sex. At present, the modification of heterogeneous materials has been using the glutaraldehyde treatment method. This method was first used in the treatment of heart valve materials by the Frenchman Carpentier in 1968...

Claims

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Application Information

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IPC IPC(8): A61L27/00
Inventor 胡盛寿周建业王德
Owner 胡盛寿
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