Resist calcification modified method of heterogeneity biological organization material

Abstract

The present invention discloses a method of calcification-resisting modifying for the heterologous biology tissue material, in the method the tannic acid solution is used to immerse the tissue; the concentration of the tannic acid is between 1 and 6%, the pH value of the solution is between 4.0 and 6.0, the immersing temperature is between 18 and 32 DEG C, and the immersing time is between 24 and 96 hour. The method of the invention can simultaneously crosslink the collagen fiber, the elastic fiber and glycosaminoglycan in the cell epimatrix of the tissue material, reduce the degradation when the tissue is implanted to the body, compensate the insufficiency that the pentanedial only crosslinks the collagen fiber and remarkably increase the calcification-resisting capability of the material; has the effect of antibacterial and reducing the protein antigenicity, and helps to increase the calcification-resisting capability and bioavailability of the material. The intercrossed tissue material has the advantages of good biostability, reinforced biomechanical property and long service lifetime.

Description

technical field [0001] The invention relates to an anti-calcification modification method for biological tissue materials, in particular to an anti-calcification modification method for homogeneous or heterogeneous biological tissue materials. Background technique [0002] In cardiac surgery, in most cases, it is necessary to implant various repair materials to replace diseased tissues and organs and restore their functions. According to the source of implant materials, it can be roughly divided into two categories: artificial materials and biological materials, and biological materials usually need to be chemically modified to remove their immunogenicity, increase the ability to resist enzymatic hydrolysis, and improve the durability of materials in the body. sex. At present, the modification of heterogeneous materials has been using the glutaraldehyde treatment method. This method was first used in the treatment of heart valve materials by the Frenchman Carpentier in 1968...

AI Technical Summary

Problems solved by technology

[0003] However, glutaraldehyde treatment also has its own shortcomings. First, it only has a certain cross-linking effect on collagen fibers, but has no cross-linking effect on other components such as elastic fibers and proteoglycans; Calcification

The calcified tissue will become hard and brittle, which will affect the activity of the tissue and be easily torn and perforated, resulting in poor durability of the biomaterial. The patient must undergo a second operation to remove the calcified tissue, which not only poses a certain risk but also increases the patient’s Unnecessary pain, so the glutaraldehyde modification treatment method largely limits the application of biomaterials

How to alleviate the calcification of glutaraldehyde-crosslinked biomaterials has been the focus of research in the past two decades, from removing lipids and cell debris that cause calcification in tissues, neutralizing residual glutaraldehyde in tissues and competitively inhibiting calcium ion absorption Equal angles have also found some effective treatment methods, but their operations are complicated, and their long-term effects are still under observation

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