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Composite nanofiber pad, hydrogel/sponge dressing, preparation method and application

A technology of composite nanofibers and hydrogels, applied in the field of medical materials, can solve problems such as weak fiber strength, and achieve the effect of overcoming weak strength, facilitating adhesion and improving strength

Active Publication Date: 2019-08-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Another object of the present invention is to provide a method for preparing a composite nanofiber mat, which provides mechanical support for the decellularized matrix nanofibers, overcomes the problem of weak strength of the simply decellularized matrix nanofibers, and simultaneously It can maintain the internal micro-nano bionic structure, which is conducive to cell adhesion, proliferation and migration, and provides a good carrier for drugs

Method used

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  • Composite nanofiber pad, hydrogel/sponge dressing, preparation method and application
  • Composite nanofiber pad, hydrogel/sponge dressing, preparation method and application
  • Composite nanofiber pad, hydrogel/sponge dressing, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of composite nanofiber mats

[0046] (1) Preparation of decellularized matrix. Take 20m of pig-derived skin tissue 3 , cut into small pieces, washed with warm water, soaked in 3% tritonX-100 for 12 hours, washed with deionized water for 15 minutes three times, treated with 4% sodium deoxycholate solution for 24 hours, washed with deionized water Neat for 15 minutes three times, vacuum freeze-dry for 2 days, the temperature of the freeze dryer is -50°C, and the pressure is 0.1MPa. Take ethanol / dichloromethane (volume ratio 2:1) equivalent to 10 times the volume of decellularized tissue and organ matrix to degrease the above-mentioned vacuum freeze-dried matrix twice, each time for 1 day, wash with deionized water for 3 each time for 15 minutes, vacuum filtration overnight, vacuum freeze-drying for 2 days, powdered, passed through a 40-mesh sieve and stored at -40°C to obtain the decellularized matrix powder.

[0047] The source of the decellularized matrix...

Embodiment 2

[0051] Preparation of decellularized matrix full-thickness skin repair hydrogel dressing

[0052] (1) Weigh 0.01g of pepsin and mix it into 10ml, and stir in 0.01% hydrochloric acid solution for 30 minutes to obtain a stirred liquid. After the stirring is completed, add 0.1 g of the decellularized matrix powder in Example 1 to the stirred liquid to obtain Digestion solution, continue to stir and digest for 24 hours, add the obtained enzymolysis solution into a centrifuge tube, place the centrifuge tube in a high-speed centrifuge at 30,000rpm, 4°C for 45 minutes at high speed, centrifuge twice, take the supernatant with 0.1 % NaOH to adjust the pH value of the enzymolysis solution to be greater than 8, and then adjust the pH value to 7-7.5 with HCl to obtain a pre-gel solution;

[0053] (2) At 4°C, add 10xPBS to the neutralized pregel solution, apply it to the composite nanofiber mat of Example 1, and place it in a 37°C incubator for 10 minutes to form a gel , to obtain a dece...

Embodiment 3

[0055] Preparation of decellularized matrix full-thickness skin repair sponge dressings

[0056] Weigh 0.01 g of pepsin, mix pepsin into 10 ml of 0.01% hydrochloric acid solution and stir for 30 minutes, add 0.1 g of the decellularized matrix powder of Example 1 after the stirring is completed, to obtain a digestive solution, continue stirring and digesting for 24 hours, Add the obtained lysate into a centrifuge tube, place the centrifuge tube in a high-speed centrifuge, centrifuge at 30,000 rpm and 4° C. for 45 minutes, centrifuge twice, and adjust the pH value of the lysate to 7 with 0.1% NaOH. The obtained lysate was coated on the composite nanofiber mat of Example 1, and vacuum freeze-dried at -50° C. and 0.1 Pa for 3 days to obtain a decellularized matrix full-thickness skin repair sponge dressing.

[0057] In other embodiments of the present invention, the preparation of the decellularized matrix full-thickness skin repair sponge dressing can also be prepared by the foll...

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Abstract

The invention discloses a composite nanofiber pad, a hydrogel / sponge dressing, a preparation method and application, and relates to the technical field of medical materials. The composite nanofiber pad comprises a receiving plate. A decellularized matrix and a high molecular material are compounded on the receiving plate, and the adding ratio of the decellularized matrix to the high molecular material is 1:1 to 1:6. The composite nanofiber pad is of a micro nanofiber structure. The hydrogel / sponge dressing can be used for loading components promoting regeneration and repair of wound surfaces,such as nutrients and drugs, hydrogel / sponge has high biocompatibility, and obvious cytotoxicity is avoided; the hydrogel / sponge dressing is soft, and can tightly cover all the wound surfaces to resist bacterial invasion and avoid wound infection.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a composite nanofiber pad, a hydrogel / sponge dressing, a preparation method and application. Background technique [0002] Wound refers to the place where the injury is ruptured, mostly referring to the skin, muscle, mucous membrane, etc. of humans or other animals. Wounds can generally be divided into acute wounds and chronic refractory wounds. Wound healing can generally be divided into four unique but overlapping stages, namely coagulation / hemostasis, inflammatory response / migration, proliferation / vascularization / regeneration, and tissue remodeling. Normal wound healing generally takes about three weeks, but chronic difficult-to-heal wounds will prolong the wound healing time due to their susceptibility to bacterial infection. Exposure of human wounds is often prone to bacterial infection. At the same time, in order to meet the needs of topical medication, moisturiz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/26A61L15/28A61L15/32A61L15/42A61L15/44A61L15/46
CPCA61L15/26A61L15/28A61L15/325A61L15/40A61L15/42A61L15/425A61L15/44A61L15/46A61L2300/404A61L2300/412A61L2400/12C08L67/04C08L5/08
Inventor 全大萍吴嘉辉邓荣力白莹杜钊夷饶子龙吴泽佳徐毅炜
Owner SUN YAT SEN UNIV
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