Preparation method of biogel and biogel prepared by preparation method

A biological gel and biological technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problem of inactivation of active cytokine components, and achieve the effect of good content, mild conditions, and good temperature sensitivity

Inactive Publication Date: 2021-03-26
北京帝康医药投资管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to maintain the pH in an acidic environment when gastric enzymes perform digestion, which can easily cause the active cytokine components in the extracellular matrix to be inactivated

Method used

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  • Preparation method of biogel and biogel prepared by preparation method
  • Preparation method of biogel and biogel prepared by preparation method
  • Preparation method of biogel and biogel prepared by preparation method

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[0034]The invention discloses a preparation method of the biogel and the prepared biogel, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0035] In this application, the meanings of the abbreviations are as follows:

[0036] SDS: sodium dodecyl sulfate;

[0037] PBS: phosphate buffer saline;

[0038] DNase: deoxyri...

Embodiment 1

[0081] Example 1 Decellularization of omentum derived from miniature pigs

[0082] Fresh minipig peritoneal omentum tissues (purchased from slaughterhouses) were taken and washed 3 times in PBS. The cleaned tissues were soaked in 2L of degreasing solution for 24 hours by shaking, and the shaking frequency was 200r / min. Subsequently, the fat-removed omentum tissue was washed three times in PBS, and soaked in 1L of decellularized solution I for 8 hours with shaking at a frequency of 150r / min. Subsequently, the omentum tissue treated with decellularization solution I was washed 3 times in PBS, and soaked in 1L decellularization solution II for 8 hours with shaking at a frequency of 150r / min. Subsequently, the omentum tissue treated with decellularization solution II was washed 3 times in PBS, and soaked in 1L decellularization solution III for 8 hours with shaking at a frequency of 150r / min. Finally, the omentum tissue treated with decellularization solution III was washed with...

Embodiment 2

[0083] The preparation of embodiment 2 omentum acellular matrix biogel

[0084] Add 10 g of the washed and shredded omentum acellular matrix to the digestion bottle containing the above digestion solution, then add 100 ml of the digestion solution, cover it and put it on a magnetic stirrer to stir at a speed of 100 rpm. The magnetic stirrer and The digestion bottles were placed together in a 37°C incubator for 30-45 minutes of continuous digestion, and then 2 mL of serum was added to terminate the digestion reaction. The volume ratio of serum to digestion solution was 0.1-0.2:10. The digestive juice was collected to obtain the liquid omentum acellular matrix biogel. Adjust the omentum acellular matrix concentration to 20 mg / mL, and form a gel-like omental acellular matrix biogel at 37°C for 5-10 minutes ( figure 1 ).

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Abstract

The invention relates to the field of tissue engineering and biomedical materials, in particular to a preparation method of biogel and the biogel prepared by the preparation method. The method comprises the following steps: soaking an omentum by using a buffer solution containing lauryl sodium sulfate and DNA enzyme for decellularization treatment; and digesting decellularized omentum tissue by using the combined digestive enzyme liquid in combination with a digestion bottle with a rotor, and preparing the omentum decellularized matrix biogel. According to the invention, the decellularized matrix biogel is prepared by adopting the combined digestive enzyme liquid and a special digestion method thereof. Compared with the existing method, the method disclosed by the invention is milder in condition, and the obtained omentum decellularized matrix biogel can well maintain the content of various active cell factors, has good temperature sensitivity, and can be converted into the gel state from liquid state under the condition of 37 DEG C within 5-10 minutes, so that the hydrogel can be used as an injectable carrier material to be applied to cell therapy of different diseases.

Description

technical field [0001] The invention relates to the field of tissue engineering and biomedical materials, in particular to a preparation method of biogel and the prepared biogel. Background technique [0002] Injectable hydrogels have a wide range of applications in tissue engineering and regenerative medicine. Hydrogel is a hydrophilic polymer network containing a large amount of water. Due to the physical cross-linking and chemical cross-linking between polymer chains, it will not dissolve in water and can only swell and maintain a certain shape. Hydrogels have good biocompatibility and water permeability, and these properties make hydrogels widely used in the field of biomedicine. Injectable hydrogels can be used in different aspects such as controlled release of bioactive molecules, delivery of cells, and as tissue scaffolding materials. Hydrogels are formed in situ in the body, which can avoid highly traumatic surgical procedures, accelerate healing, reduce patient pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/52
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/52A61L27/50A61L2430/40
Inventor 尹玉静饶义伟
Owner 北京帝康医药投资管理有限公司
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