A kind of artificial nerve graft and its preparation method

A technology for artificial nerves and grafts, applied in the field of nerve grafts, which can solve problems such as limiting accurate matching

Active Publication Date: 2021-12-07
SUN YAT SEN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latter comes from human residual limbs, and the allograft prepared by removing immunogenicity through a series of decellularization processes has a better repair effect on long-segment defects than nerve conduits, but its source of raw materials also limits its An exact match in size and function (sensory / motor) to the transplant target

Method used

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  • A kind of artificial nerve graft and its preparation method
  • A kind of artificial nerve graft and its preparation method
  • A kind of artificial nerve graft and its preparation method

Examples

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Effect test

preparation example Construction

[0026] The preparation method of artificial neurotransplate of the present invention includes:

[0027] (1) Preparation of polyester solution

[0028] Take a certain amount of degradable polyester, dissolved in an organic solvent, stirred 1 to 2 anime, fully dissolved.

[0029] Degrading polyester includes polylactic acid, polylactic acid-trimethylene carbonate (P (La-TMC)), polyvi acid, poly (D, L-lactic acid-CO-glycolic acid), poly (D, L-lactic acid) -CO-caprolactone), poly (d, L-lactic acid-CO-trimethylene carbonate), etc.

[0030] Organic solvents include 2,2,2-trifluoroethanol, hexafluoropropyl alcohol, chloroform, dichloromethane, and the like.

[0031] (2) Electrostatic spinning processing neural sleeve

[0032] according to figure 1 The above polyester solution is transferred to the syringe and placed on the injection pump to perform electrostatic spinning processing.

[0033] Among them, the injection rate of the solution is 0.5 to 4 ml / h; the injection needle injector ...

Embodiment example 1

[0052] Preparation of the case of a microchannel decellular matrix (sour source, PDNM) neurotransplate

[0053] P (LA-TMC) 70 / 30 is dissolved in 2,2,2-trifluoroethanol in the above method, and the concentration of 15% w / v; according to the above steps 1 to 3, it is prepared for electrospinning. A neural sleeve of 2 mm inner diameter and about 0.25 mm thick.

[0054] Pork source ambient decellular matrix (PDNM) was digested, pH, solubized by digestion, tuning pH, sol (La-TMC) in the above steps 6 to 9 according to the above steps 4 to 5; PDNM sol The 70 / 30 nerve sleeve, and the liquid nitrogen is used as a cold source to oriene freeze crystals, and the artificial nerve plant is finally foundated at -20 ° C.

[0055] Its scanning electron microscope picture image 3 The neurotrophic is composed of two parts, including the inner layer such as Figure 4 As shown, a decellular matrix having a directional microchannel having a diameter of 20-100 μm, an outer layer such as Figure 5 As sh...

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Abstract

The preparation method of a kind of artificial nerve graft of the present invention comprises: (1) take the degradable polyester of certain quality, dissolve in organic solvent, make polyester solution; (2) transfer polyester solution in the syringe, And place it on a syringe pump, and perform electrospinning processing to obtain the nerve sleeve; (3) prepare the decellularized matrix digestion solution; (5) prepare the decellularized matrix sol; (6) use the perfusion equipment to decellularize The matrix sol is perfused into the nerve sleeve; (7) The nerve sleeve perfused with the decellularized matrix sol is placed in an environment of 37° C. for 10 to 30 minutes, so that the self-assembly of the decellularized matrix undergoes a sol-gel transition, and the decellularized matrix is ​​prepared. cell matrix gel; (8) directional freeze-crystallization of decellularized matrix gel; (9) lyophilization of directional freeze-crystallized decellularized matrix gel to prepare artificial nerve graft.

Description

Technical field [0001] The present invention relates to the field of neurotrophic plant, and more particularly to artificial nerve plants and methods of preparation. Background technique [0002] When the neurodal injury in defective defects, the neural graft is often required to be repaired by bridgeing the two ends of the defect. The effect of autologous nerves is the best, but it is facing the shortcomings of the inadequate donor and the secondary injury of the donor site, so the application of artificial nerve plants has been focused. [0003] At present, artificial nerve plants used in clinical practice are mainly divided into two categories: neural catheter (including casing, sheath, film, etc.) and decellular nerves. Among them, the former is mainly manually synthetic or collagen-processed hollow tubular structure, its structure, components are far from the neuromatoid, and the clinical application is limited (<20 mM or less defect). The latter is from the human body re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/52
CPCA61L27/18A61L27/3633A61L27/52A61L2430/32C08L67/04
Inventor 全大萍饶子龙陈诗浩白莹周晶朱庆棠刘小林杨习锋曾晨光
Owner SUN YAT SEN UNIV
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