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A kind of artificial blood vessel and preparation method thereof

An artificial blood vessel and pipeline technology, which is applied in anticoagulant treatment, packaged food, prosthesis, etc., can solve the problems of poor mechanical properties of artificial blood vessels, weak anticoagulant function, low patency rate of blood vessels, etc., and achieve good clinical application prospects , good anticoagulant function, and high patency rate

Active Publication Date: 2019-05-31
海迈医疗科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above defects or improvement needs of the prior art, the present invention provides an artificial blood vessel and its preparation method, the purpose of which is to obtain an artificial blood vessel by decellularizing and covalently binding heparin to the autologous tissue pipeline, thereby Solve the technical problems of poor mechanical properties, weak anticoagulant function and low vascular patency rate caused by immune reaction in the prior art artificial blood vessels

Method used

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  • A kind of artificial blood vessel and preparation method thereof
  • A kind of artificial blood vessel and preparation method thereof
  • A kind of artificial blood vessel and preparation method thereof

Examples

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Effect test

preparation example Construction

[0047] The concrete steps of artificial blood vessel preparation method of the present invention are as follows:

[0048] (1) Preparation of autologous tissue pipeline

[0049] Cut the Teflon (Teflon) tube whose diameter matches the inner diameter of the blood vessel to be replaced to an appropriate length, sterilize it with 75% alcohol for 30 minutes, place it in the subcutaneous tissue, and take it out after 2 to 5 weeks, preferably 4 weeks. The surrounding newborn tissue was taken out, and the Teflon tube was extracted to obtain the autologous tissue pipeline. The thickness of the autologous tissue pipeline and the final artificial vascular product corresponding to 4 weeks are the most suitable, and the corresponding artificial vascular product has the best performance.

[0050] (2) Decellularization treatment

[0051] (2-1) Configuration of decellularization reagent: 3-[(3-cholamidopropyl)-diethylamine]-propanesulfonic acid (abbreviated as CHAPS reagent), EDTA.Na2, NaCl,...

Embodiment 1

[0064] (1) Preparation of autologous tissue pipeline

[0065] Cut a Teflon tube with a diameter matching the inner diameter of the blood vessel to be replaced to a suitable length, sterilize it with 75% alcohol for 30 minutes, and place it in the subcutaneous tissue. After 4 weeks, take out the subcutaneous Teflon tube together with the surrounding new tissue, and draw it out. In addition to the Teflon tube, an autologous tissue tube was obtained, the thickness of which was 324.1±57.4 microns (n=6, n is the number of test samples, and n has the same meaning below).

[0066] (2) Decellularization treatment

[0067] (2-1) Configuration of decellularization reagent

[0068]

[0069] According to the formula in the above table, add the accurately weighed reagent into a clean wide-mouth glass bottle, shake on the shaker for 2-3 hours to fully dissolve the reagent, at this time the solution becomes clear, prepare the decellularization reagent, and store it at 4°C for later use ...

Embodiment 2

[0083] (1) Preparation of autologous tissue pipeline

[0084] Cut the Teflon tube whose diameter matches the inner diameter of the blood vessel to be replaced to an appropriate length, sterilize it with 75% alcohol for 30 minutes, and place it in the subcutaneous tissue. After 2 weeks, take out the subcutaneous Teflon tube together with the surrounding new tissue, and draw it out. In addition to Teflon tubes, autologous tissue tubes were obtained with a thickness of 154.3±56.4 microns (n=6).

[0085] (2) Decellularization treatment

[0086] (2-1) Configuration of decellularization reagent

[0087]

[0088] According to the formula in the above table, add the accurately weighed reagent into a clean wide-mouth glass bottle, shake on the shaker for 2-3 hours to fully dissolve the reagent, at this time the solution becomes clear, prepare the decellularization reagent, and store it at 4°C for later use .

[0089] (2-2) Decellularization of autologous tissue pipeline

[0090]...

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Abstract

The invention discloses an artificial blood vessel and a preparation method thereof. In the present invention, the cellular components of the autologous tissue pipeline are removed by using 3-[(3-cholamidopropyl)-diethylamine]-propanesulfonic acid reagent chemical decellularization method, and then the heparin is combined in a covalent manner To the surface of the autologous acellular matrix tubing, an artificial blood vessel is obtained. The artificial blood vessel prepared by this method has no immunogenicity, short preparation period, low cost, can be prepared into different inner diameters, has good anticoagulant function, does not need complicated in vitro preparation process, high patency rate, and cells can effectively enter the vessel wall Participating in remodeling, completely superior to previous allogeneic or xenogeneic acellular matrix pipelines, has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the field of tissue engineering blood vessels, and more specifically relates to an artificial blood vessel and a preparation method thereof. Background technique [0002] At present, autogenous blood vessels such as the great saphenous vein and the internal mammary artery are the most important graft vessels clinically used in coronary artery bypass surgery and other small-caliber vessel bypass surgery. The two small-bore blood vessels of acellular matrix pipelines currently approved by the US Food and Drug Administration (FDA) for clinical trials are: [0003] (1) For the first time, Nicolas L'Heureux et al. applied the acellular matrix tissue engineering blood vessel without artificial synthetic materials in clinical practice. The patency rate of venous fistula phase I clinical trial was 78% (7 / 9) at 1 month, and 60% (5 / 8) at 6 months. Although Nicolas L’Heureux and others have prepared autologous acellular matrix pipelines...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/50A61L27/36A61L33/08A61L33/00
CPCA61L27/36
Inventor 邱雪峰董念国王滔
Owner 海迈医疗科技(苏州)有限公司
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