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Low-immunogenic bone defect part filling material and preparation method thereof

A technology for filling materials and bone defects, applied in medical science, prostheses, tissue regeneration, etc., can solve the problems of safety hazards, strong immunogenicity, etc., and achieve the goal of preventing fiber formation, reducing immunogenicity, and ensuring complete recovery Effect

Active Publication Date: 2019-03-29
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing non-autologous bone filling materials have achieved good results in mechanics and biotherapeutics, they all have strong immunogenicity, and there are potential safety hazards in long-term use, which limits their clinical application.

Method used

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  • Low-immunogenic bone defect part filling material and preparation method thereof
  • Low-immunogenic bone defect part filling material and preparation method thereof
  • Low-immunogenic bone defect part filling material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of filling material for bone defect with low immunogenicity

[0023] Preparation method 1: According to the composition of each experimental group of filling materials for low-immunogenic bone defects in Table 1, weigh each component, crush the bone decellularized matrix according to the aseptic method, and mix with heparin-loaded high-affinity cells The heparin-poloxamer gel of the growth factor was mixed evenly, and the aminated aloe polysaccharide-glyceraldehyde gel was added, and mixed evenly to form a uniform semi-solid gel.

[0024] Preparation method 2: According to the composition of each experimental group of filling materials for low-immunogenic bone defects in Table 1, weigh each component, crush the bone decellularized matrix according to the aseptic method, and coagulate with aminated aloe polysaccharide-glyceraldehyde Mix the gel evenly, add heparin-poloxamer gel loaded with heparin high-affinity cell growth factor, and mix evenly to ...

Embodiment 2

[0033] Example 2 In vivo evaluation of the application effect of bone defect filling materials in each group

[0034] (1) Animal model of rabbit radius defect

[0035] The degree of bone defect in the bone defect model is based on the concept of critical bone defect proposed by Schmitz et al., that is, when the bone defect reaches 1.5 times the diameter of the long bone, the body cannot heal itself, resulting in bone defect, while the bone defect smaller than this multiple can heal itself .

[0036] Rabbit radial defect model: New Zealand white rabbits weighing 2.5-3kg were taken. After anesthesia, the blood vessels and tendons at the radius were separated, the radius was exposed, the radius was sawn off until it was 1.5 times the section diameter, and the radial bone defect was confirmed by Micro-CT images. Whether the defect position is correct and whether the gap is neat.

[0037] (2) In vivo application effect of each group of filling materials

[0038] Using the rabbit...

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Abstract

The invention provides a low-immunogenic bone defect part filling material. The filling material is composed of aminated aloe polysaccharide-glyceraldehyde gel, heparin high affinity cytokine-entrapped heparin-poloxamer gel and a bone acellular matrix, and the mechanical strength and the degradation speed of the bone defect part filling material are controlled by changing the ratio of the components. The low-immunogenic bone defect part filling material overcomes the shortcomings and deficiencies of high immunogenicity in the prior art, provides a novel bone defect part filling material with low immunogenicity and multiple functions of bone conduction, bone induction, bone formation and mechanics support, provides the best physicochemical and biological micro-environments for bone regeneration, effectively guides the directional arraying of cells, has good mechanical properties, and can quickly repair bone defect parts.

Description

technical field [0001] The invention relates to a bone defect filling material, in particular to the composition, preparation and application of a bone defect filling material with low immunogenicity. Background technique [0002] The clinical incidence of bone defect is relatively high, and its treatment is still a difficult problem in orthopedics. Bone defects can be treated with bone grafting, tissue engineering technology, membrane-guided tissue regeneration technology, gene therapy, etc. However, it is still common to treat bone defects with bone grafting clinically. [0003] Autologous bone transplantation has the advantages of low immunogenicity and good inductivity, but the source of materials for autologous bone grafting is limited. In addition, new trauma will inevitably be added when performing autologous bone grafting, and there may be a problem in the donor area after surgery. A series of complications, so autologous bone transplantation is difficult to be wid...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/20A61L27/22A61L27/36A61L27/52A61L27/54A61L27/58
CPCA61L27/18A61L27/20A61L27/227A61L27/3608A61L27/365A61L27/52A61L27/54A61L27/58A61L2300/232A61L2300/414A61L2300/42A61L2300/602A61L2430/02C08L5/00C08L5/10C08L89/00C08L71/02
Inventor 赵应征鲁翠涛徐荷林姚情夏清海诸葛德力杨外庚
Owner WENZHOU MEDICAL UNIV
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