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Cartilage acellular matrix and preparation method thereof

A decellularized matrix and decellularized technology, applied in the field of cartilage decellularized matrix and its preparation, can solve the problems of tissue collapse, refractory degradation, slow degradation, etc., and achieve the effect of reducing damage

Active Publication Date: 2022-07-29
NEO MODULUS SUZHOU MEDICAL SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the decellularized cartilage matrix is ​​obtained by crushing the material itself and combining it with other materials to reconstruct the cartilage tissue; the method of mixing the crushed material with other polymer materials for reconstruction faces a major problem that polymer materials are not available. Avoid rapid biodegradation in vivo. With the degradation of polymer materials, the tissue body may face collapse and other situations; and most of the materials sought for rhinoplasty are materials that degrade slowly or are difficult to degrade. The type II collagen network structure of cartilage materials can meet such degradation requirements. Therefore, obtaining cartilage decellularized matrix without destroying the structure of cartilage itself will become the research direction of allogeneic cartilage materials.

Method used

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  • Cartilage acellular matrix and preparation method thereof
  • Cartilage acellular matrix and preparation method thereof
  • Cartilage acellular matrix and preparation method thereof

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preparation example Construction

[0035] In order to obtain the cartilage acellular matrix without destroying the structure of the cartilage itself, the present invention provides a preparation method of the cartilage acellular matrix, the preparation method comprising the following steps:

[0036] S1: take mammalian cartilage, pretreat the cartilage to obtain clean cartilage;

[0037] S2: Treat the clean cartilage with an enzyme solution to open the pore structure of the clean cartilage surface to obtain the cartilage material A;

[0038] S3: using a non-ionic reagent to remove the cell components in the superficial layer of the cartilage material A to obtain the cartilage material B;

[0039] S4: using an ionic reagent to preliminarily remove the cellular components in the deep layer of the cartilage material B to obtain the cartilage material C;

[0040] S5: using a zwitterionic reagent to remove the cell components in the deep layer of the cartilage material C twice to obtain the cartilage material D;

...

Embodiment 1

[0075] The present embodiment provides a method for preparing a cartilage acellular matrix, comprising the following steps:

[0076] S1: Take porcine rib cartilage, use a scalpel to remove the flesh on the cartilage surface, scrape the periosteum on the rib cartilage surface, and obtain a white cartilage material without any fleshy residue and pale pink periosteum on the surface; purified water was used for the obtained cartilage material. For cleaning, first use running water to rinse the cartilage until the cartilage is clean, then use purified water to shake and clean the cartilage material 4 times for 30 minutes each time at 37°C, a material-liquid ratio of 1:10, and a rotational speed of 200 r in an air-bath constant temperature shaking box. get clean cartilage;

[0077] S2: Digest clean cartilage with trypsin solution with a mass concentration of 1%, shake for 8 hours in an air bath constant temperature shaking box at 37° C., under the conditions of a solid-liquid ratio ...

Embodiment 2

[0088] The present embodiment provides a method for preparing a cartilage acellular matrix, comprising the following steps:

[0089] S1: Take the bovine rib cartilage, use a scalpel to remove the flesh on the cartilage surface, scrape the periosteum on the surface of the rib cartilage, and obtain a white cartilage material without any fleshy residue and pale pink periosteum residue on the surface; purified water was used to carry out the obtained cartilage material. For cleaning, first use running water to rinse the cartilage until the cartilage is clean, then use purified water to shake and clean the cartilage material 4 times for 30 minutes each time at 37°C, a material-liquid ratio of 1:10, and a rotational speed of 200 r in an air-bath constant temperature shaking box. get clean cartilage;

[0090] S2: Use a trypsin solution with a mass concentration of 1% to digest the clean cartilage, and shake it for 12 hours in an air-bath constant temperature shaking box at 37° C., a ...

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Abstract

The invention provides a cartilage acellular matrix and a preparation method thereof. The preparation method comprises the following steps: treating clean cartilage by using an enzyme solution; a non-ionic reagent is used for removing cell components in the shallow layer; carrying out preliminary removal on deep cell components by using an ionic reagent; carrying out secondary removal on deep cell components by using a zwitterionic reagent; cleaning with purified water to obtain a decellularized cartilage material; performing degreasing treatment by using an organic solvent to obtain a degreased cartilage material; carrying out virus inactivation on the degreased cartilage material to obtain an inactivated cartilage material; and freeze-drying the inactivated cartilage material to obtain the cartilage acellular matrix. According to the preparation method of the cartilage decellularized matrix, the cartilage material does not need to be destroyed and reconstructed, the cartilage decellularized matrix is obtained under the condition that the structure of cartilage is not destroyed, and a type II collagen network of the cartilage material is well protected.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a cartilage acellular matrix and a preparation method thereof. Background technique [0002] Rhinoplasty has developed into a relatively mature surgical procedure in plastic surgery. With the development of the times, many patients no longer seek rhinoplasty because of facial defects. The beauty of the heart has made the medical beauty industry develop rapidly, and the market demand for rhinoplasty is increasing. Therefore, a suitable nasal cartilage graft will become a key requirement for the future development of rhinoplasty technology. [0003] Currently widely used rhinoplasty prostheses include expanded polytetrafluoroethylene (ePTFE), solid silica gel, hyaluronic acid, artificial bone materials, etc. However, due to the limitations of these materials, the sources of rhinoplasty materials currently used are mostly in autologous cartilage. However, autologous ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3612A61L27/3633A61L27/3654A61L27/3687A61L27/3691A61L27/50A61L2430/40A61L2430/06
Inventor 李啸宏陈维明刘昌俊
Owner NEO MODULUS SUZHOU MEDICAL SCI TECH CO LTD
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