Biological cornea prepared from swimming bladder as well as preparation method and application of biological cornea

Abstract

The invention discloses a biological cornea prepared from swimming bladders as well as a preparation method and application of the biological cornea. Fresh swimming bladders are subjected to impurity removal, degreasing, desugaring and freeze thawing treatment, and then a decellularized solution is added to obtain a decellularized matrix of the swimming bladders; and carrying out collagenous fiber reconstruction treatment on the acellular matrix by using an alcohol-water mixed solution containing a light transmission regulator to obtain the artificial biological cornea. The swim bladder is used as a material for preparing the biological cornea, the obtained biological cornea is very close to a natural cornea in component, has excellent optical properties and excellent mechanical properties, shows excellent biocompatibility and is free of cytotoxicity through in-vitro cell culture, and cornea cells can be normally adhered and grow on the surface of the material, so that the biological cornea can be used for preparing the biological cornea. And a compact cell layer is formed and can be used as a medical material in corneal transplantation and corneal lamella repair. The carrier stent is wide in raw material source, low in production cost, convenient to store and use and capable of meeting the requirements of a large number of carrier stents of tissue engineering corneas.

Description

technical field [0001] The invention relates to the field of medical materials in medicine and bioengineering, in particular to a biological cornea prepared from swim bladders, a preparation method and application thereof. Background technique [0002] Corneal disease is one of the world's major eye diseases and an important cause of blindness. The incidence rate in many countries is increasing year by year, and it has become the second leading cause of blindness in some developing countries in recent years. The lesions of the corneal stroma are often caused by the destruction of the arrangement of the stromal collagen lamina. It takes several months or even years for the corneal stroma to heal, and the healing wound will also produce scars that affect the clarity of the cornea. Therefore, stroma lesions usually affect vision. Corneal diseases of the stromal layer include central corneal thinning and conical keratoconus protruding forward; most of them show autosomal domin...

AI Technical Summary

Problems solved by technology

At present, the carrier scaffold materials used for the construction of tissue engineering artificial cornea mainly include allogeneic cornea (human), xenogeneic acellular cornea (pig), natural biological materials, and artificial synthetic materials, but there are certain shortcomings and deficiencies: xenogeneic acellular cornea Corneal stroma, such as porcine cornea, will destroy the original structure and composition of the stroma during the decellularization process, affecting its transparency and mechanical strength. It may also cause inflammatory reactions and decrease in transparency after transplantation; natural biological materials such as collagen, chitosan, fibrin The degradation rate of scaffolds made of these materials is too fast and the mechanical strength is not good; artificial synthetic materials sometimes face the problem of poor biocompatibility, and their degradation products may have certain toxic effects on organisms.

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