Tissue engineering scaffold for urethra reconstruction and preparation method thereof

A technology of tissue engineering scaffold and urethra, which is applied in the textile industry and the field of material science to achieve excellent mechanical properties, reduce production costs, and avoid injuries

Active Publication Date: 2013-10-30
SHANGHAI SHUIXING HOME TEXTILE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no literature reports or patents related to the research and production of tissue engineering scaffolds prepared by combining the acellular matrix under the small intestinal mucosa or the submucosal acellular matrix of the bladder with regenerated silk fibroin.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for preparing a tissue engineering scaffold for urethral reconstruction, the steps of which are:

[0036] (1) using silkworm cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 30%;

[0037]The preparation steps of the regenerated pure silk fibroin aqueous solution are as follows: after the silkworm cocoons are degummed with a 0.3% sodium carbonate aqueous solution by mass percentage, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.0 mol / L to obtain the regenerated silk fibroin-lithium bromide Aqueous solution; the solution is centrifuged, filtered, dialyzed and concentrated to obtain a 30% aqueous solution of regenerated silk fibroin by mass;

[0038] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovine serum albumin, wherein the mass concentration of vascul...

Embodiment 2

[0042] A method for preparing a tissue engineering scaffold for urethral reconstruction, the steps of which are:

[0043] (1) using tussah cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 33%;

[0044] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: degumming tussah silkworm cocoons with a 0.4% aqueous sodium carbonate solution by mass percentage, and dissolving them in an aqueous lithium bromide solution with a molar concentration of 9.1 mol / L to obtain a regenerated silk fibroin-lithium bromide aqueous solution ; This solution is centrifuged, filtered, dialyzed and concentrated to obtain a regenerated silk fibroin aqueous solution with a mass percentage of 33%;

[0045] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovine serum albumin, wherein the mass concentration of v...

Embodiment 3

[0049] A method for preparing a tissue engineering scaffold for urethral reconstruction, the steps of which are:

[0050] (1) using castor cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 35%;

[0051] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: degumming the castor silkworm cocoons with a 0.5% aqueous sodium carbonate solution by mass percentage, and then dissolving them in a lithium bromide aqueous solution with a molar concentration of 9.2 mol / L to obtain the regenerated silk fibroin-lithium bromide Aqueous solution; the solution is centrifuged, filtered, dialyzed and concentrated to obtain a regenerated silk fibroin aqueous solution with a mass percentage of 35%;

[0052] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovine serum albumin, wherein the mass concentrat...

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Abstract

The invention relates to a tissue engineering scaffold for urethra reconstruction and a preparation method thereof. The preparation method comprises the steps of: (1) preparing a regenerated silk fibroin aqueous solution; (2) preparing a vascular endothelial growth factor and bovine serum albumin aqueous solution; (3) mixing the solutions prepared in the steps (1) and (2) to prepare a regenerated silk fibroin solution containing the vascular endothelial growth factor; and (4) preparing the tissue engineering scaffold by adopting an electrostatic spinning technology with the solution prepared in the step (3) as a spinning liquid and a decellularized matrix as a base layer. According to the invention, the tissue engineering scaffold is of a double-layer structure composed of a decellularized matrix base layer and a functional layer, and the functional layer is a fibrofelt which is formed by regenerated silk fibroin fiber mixed with the vascular endothelial growth factor and bovine serum albumin and has a three-dimensional staggered network structure characteristic. The tissue engineering scaffold prepared by the method not only has excellent mechanical properties, but also can be early and quickly vascularized in vivo, and can be used for repairing long-segment urethrostenosis.

Description

technical field [0001] The invention belongs to the technical field of textile industry and material science, and in particular relates to a tissue engineering scaffold for urethral reconstruction and a preparation method thereof. The regenerated silk fibroin solution containing vascular endothelial growth factor is used as the spinning liquid, and the urethra is spun by electrospinning The process sprayed it on the acellular matrix to prepare a tissue engineering scaffold for urethral reconstruction. Background technique [0002] Long-segment complex urethral strictures will not only cause local complications such as urinary extravasation, urinary fistula, and false tract, but in severe cases, it will even affect other organs of the urinary system and cause systemic lesions, seriously affecting the quality of life of patients. In the past, the replacement therapy using a variety of autologous tissues as reconstruction materials showed good application prospects in the treat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/36A61L27/34A61L27/22A61L27/54
Inventor 张耀鹏范苏娜邵惠丽杭怡春潘辉徐月敏冯超谢敏凯
Owner SHANGHAI SHUIXING HOME TEXTILE
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