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Injectable bioactive composite conductive hydrogel as well as preparation method and application thereof

A composite conductive and bioactive technology, applied in pharmaceutical formulations, drug delivery, medical science, etc., can solve the problem that the microenvironment of cell growth cannot be simulated well, the conductive hydrogel does not have biological activity, and the cell growth promotion effect is not obvious. and other problems, to achieve the effect of improving cell adhesion and biological activity, excellent biocompatibility, and easy realization.

Active Publication Date: 2022-05-17
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrogel material is a conductive hydrogel without biological activity and has poor biocompatibility.
In general, although the existing conductive hydrogel materials have a high modulus, their biological activity is low, and the promotion effect on cell growth is not obvious, and due to the brittleness of their conjugated structure, the resulting materials have poor mechanical properties. ; At the same time, the natural or synthetic biological materials used do not have tissue-specific biological activity, and cannot better simulate the cell growth microenvironment in the original living tissue, resulting in insufficient biological performance

Method used

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  • Injectable bioactive composite conductive hydrogel as well as preparation method and application thereof
  • Injectable bioactive composite conductive hydrogel as well as preparation method and application thereof
  • Injectable bioactive composite conductive hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of decellularized matrix PEDOT:PSS composite conductive hydrogel (pDNM / PEDOT:PSS composite conductive hydrogel)

[0048] (1) Preparation of freeze-dried peripheral nerve decellularized powder: fresh sciatic nerve tissue from the lower limbs of miniature pigs was decellularized with 3% Triton X-100 for 12 hours and 4% sodium deoxycholate for 24 hours, and then washed with deionized water for 3 times , this process was repeated twice to complete decellularization, then freeze-dried and chopped, and then soaked in a mixed solution of absolute ethanol and dichloromethane (V 乙醇 :V 二氯甲烷 = 1:2), stirred for 24 hours, repeated twice, and finally put into a vacuum drying oven to remove the solvent, rinsed with deionized water for 3 times, freeze-dried and pulverized again to obtain peripheral nerve decellularized powder;

[0049] (2) First dissolve pepsin with dilute hydrochloric acid to obtain a pepsin solution (1 mg / mL), then dissolve 5 mg of peripheral ...

Embodiment 2

[0056] Example 2 Preparation of pDNM / PEDOT:PSS Composite Conductive Hydrogel (0.5%pDNM+0.01%PEDOT:PSS)

[0057] The preparation method is the same as that of Comparative Example 1, except that the neutral PEDOT:PSS solution in step (7) is 5 μL, the amount of PBS added is 495 μL, and its appearance is as follows: figure 1 shown.

Embodiment 3

[0058] Example 3 Preparation of pDNM / PEDOT:PSS Composite Conductive Hydrogel (0.5%pDNM+0.025%PEDOT:PSS)

[0059] The preparation method was the same as that of Comparative Example 1, except that the neutral PEDOT:PSS solution in step (7) was 25 μL, and the amount of PBS added was 475 μL.

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Abstract

The invention belongs to the technical field of tissue engineering, and particularly relates to injectable bioactive composite conductive hydrogel as well as a preparation method and application thereof, and the composite conductive hydrogel is prepared through respective physical crosslinking of acellular matrix hydrogel and PEDOT: PSS. The composite conductive hydrogel provided by the invention has extremely high electron transmission efficiency and mechanical strength, can well reduce a microenvironment for cell growth, has excellent biocompatibility, can promote tissue regeneration, has good fluidity and injectability, is not limited by shape and size when in use, can well adhere to tissues after gelatinization, and can be used for preparing the hydrogel. Extra surgical suture is not needed; the conductivity of the hydrogel can be adjusted by adjusting the adding amount of PEDOT: PSS, electrical stimulation is provided for cells by externally adding weak current, cell proliferation, migration and functionalization can be effectively promoted, and the hydrogel is expected to be prepared into a biomedical conductive material for application.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to an injectable bioactive composite conductive hydrogel and its preparation method and application. Background technique [0002] With the development of tissue engineering, more and more biomaterials are used in the tissue regeneration process. Among all the applied biomaterials, natural biomaterials are a class of biomaterials that come from a wide range of sources and are generated under natural conditions, including natural fibers, biological tissues, structural proteins, polysaccharides, etc. Natural biomaterials have excellent biocompatibility and bioactivity, and have similar mechanical properties to tissues, which can provide structural support and adhesion sites for cell growth. [0003] Hydrogel is a kind of three-dimensional network structure rich in water. Due to the existence of cross-linked network, hydrogel can swell in water without breaking,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/16A61L27/50A61L27/52A61L27/56
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/18A61L27/16A61L27/52A61L27/50A61L27/56A61L2400/06C08L65/00C08L25/18
Inventor 白莹王赛兰饶子龙全大萍周晶
Owner SUN YAT SEN UNIV
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