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Piezoelectric stent as well as preparation method and application thereof

A piezoelectric, drug-loaded microsphere technology, applied in the fields of biomedical engineering and biomedical materials, can solve problems such as hindering the contact between micropores and organic solvents, affecting the performance of acellular matrix, affecting the removal of antigenic substances, etc., and achieves good drug loading. Drug release performance, excellent biocompatibility, and the effect of facilitating stable loading

Active Publication Date: 2021-03-16
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acellular matrix can only provide short-term release of the drug, and cannot release the drug for a long time to continuously stimulate the "focus" site, making it difficult for the acellular matrix to treat the defective tissue alone
Moreover, different decellularization methods directly affect the composition and ultrastructure of the obtained acellular matrix, thereby affecting its performance.
[0006] At present, the decellularization of bone to prepare the acellular matrix usually uses organic solvents such as chloroform and methanol mixture, and because the micropores of bone tissue are hydrophilic, this will hinder the contact between the micropores and the organic solvent, thereby affecting the potential. The removal of antigenic substances during the treatment process will affect the performance of the acellular matrix; in addition, the organic solvents adsorbed on the deep surface of the micropores during the treatment process may not be completely removed during the cleaning process, which will cause harmful substances to remain. May be harmful to human health

Method used

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  • Piezoelectric stent as well as preparation method and application thereof
  • Piezoelectric stent as well as preparation method and application thereof
  • Piezoelectric stent as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0036] (1) Preparation of porous barium titanate

[0037] The porous barium titanate used in the following piezoelectric stent preparation process was prepared by the following method:

[0038] 1.2g hexadecyltrimethylammonium bromide is dissolved in the aqueous hydrogen chloride solution of 120ml 75mol / l, obtains the first solution; 9.2ml titanium tetrachloride is dissolved in the mixed solution of 100ml ethanol and 50ml deionized water, Obtain the second solution; add the second solution, 50ml of 1.5mol / l barium chloride aqueous solution and 25g of sodium hydroxide to the first solution at intervals of 6h under continuous stirring, stop stirring after 24h, and reflux at 90°C for 5h , the product was collected by filtration, washed three times with deionized water and ethanol respectively, and filtered with suction to obtain porous barium titanate powder.

[0039] (2) Preparation of the piezoelectric scaffold

Embodiment 1

[0041] A kind of piezoelectric support, its preparation method comprises the following steps:

[0042] S1. Preparation of acellular matrix, comprising: taking trabecular bone in the subchondral area of ​​the upper limb of an 8-week-old dairy cow and washing it with high-pressure water; Soak in 0.3% sodium dodecyl sulfate in 10mM Tris buffer for 6h, wash with PBS several times until there are no bubbles; then use supercritical carbon dioxide technology to treat it, set the minimum pressure to 6MPa, the maximum pressure to 16MPa, and the system temperature to be 37°C, specifically exposed to the maximum pressure for 10 minutes, with a flow rate of 10kg / h, and then reduced to normal pressure within 6 minutes to prepare an acellular matrix with a three-dimensional porous structure;

[0043] S2. Preparation of drug-loaded microspheres, including: mixing 10 mg BMP-2 with 100 mg porous barium titanate to obtain a mixed powder of drug and porous barium titanate, and then dispersing th...

Embodiment 2

[0046] A kind of piezoelectric support, its preparation method comprises the following steps:

[0047] S1. Preparation of acellular matrix, comprising: taking trabecular bone in the subchondral region of the upper limb of a 4-week-old dairy cow and washing it with high-pressure water; Soak in 0.1% sodium dodecyl sulfate in 10mM Tris buffer for 6h, wash with PBS several times until there are no bubbles; then use supercritical carbon dioxide technology to treat it, set the minimum pressure to 8MPa, the maximum pressure to 20MPa, and the system temperature to be 37°C, specifically exposed to the maximum pressure for 12 minutes, with a flow rate of 10kg / h, and then reduced to normal pressure within 5 minutes to prepare an acellular matrix with a three-dimensional porous structure;

[0048] S2. Preparation of drug-loaded microspheres, comprising: mixing 10 mg of alendronate sodium with 40 mg of porous barium titanate to obtain a mixed powder of drug and porous barium titanate, and ...

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Abstract

The invention discloses a piezoelectric stent as well as a preparation method and application thereof, and the preparation method of the piezoelectric stent comprises the following steps: performing decellularization treatment on bone tissue organs, and then performing supercritical CO2 extraction to prepare a decellularized matrix with a three-dimensional porous structure; preparing drug-loaded microspheres; then mixing the drug-loaded microspheres with the decellularized matrix so as to enable the drug-loaded microspheres to cover the surface of the decellularized matrix and fill pores of the decellularized matrix, and carrying out heat preservation at 37-65 DEG C until the drug-loaded microspheres are bonded on the decellularized matrix so as to prepare a stent containing the drug-loaded microspheres; and then soaking the stent containing drug-loaded microspheres in a degradable piezoelectric polymer solution, taking out the stent for draining, and carrying out polarization treatment. The stent prepared by the preparation method of the piezoelectric stent can effectively delay the release rate of a drug, has osteogenic differentiation capacity and a piezoelectric response function, and can effectively promote repair and reconstruction of bone tissue.

Description

technical field [0001] The invention relates to the technical fields of biomedical engineering and biomedical materials, in particular to a piezoelectric bracket and its preparation method and application. Background technique [0002] With the continuous development of medicine, pharmacy, and biology, new drugs for various diseases emerge in an endless stream, but how to release drugs continuously and stably in the body is still a difficult problem. Regardless of oral administration or intravenous injection, there will be a "peak-valley" phenomenon in the change of blood drug concentration. If the drug concentration is too high, it will cause relatively large toxic and side effects, while if it is too low, the therapeutic effect will not be achieved. Although the emerging bioactive macromolecular drugs are highly effective, they have a short biological half-life and are easily inactivated, which is also a problem that plagues drug developers. Use appropriate biomaterials t...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/34A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/3633A61L27/365A61L27/34A61L27/50A61L27/56A61L27/54A61L27/58A61L2430/02A61L2300/62A61L2300/602A61L2300/414A61L2300/412A61L2300/216C08L67/04
Inventor 许为康李桂香周新婷黄德群
Owner GUANGDONG PROV MEDICAL INSTR INST
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