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Artificial nerve graft prepared by electrostatic spinning and preparation method and special device thereof

An electrospinning process and artificial nerve technology are applied in the field of artificial nerve grafts and their preparation, which can solve the problems of difficult matching of tissue structure and size, limited nerve sources, and long-term denervation in the transplant donor area, and achieve good use effects. , good biocompatibility

Inactive Publication Date: 2010-03-10
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In autologous nerve transplantation, it has not been widely used clinically because of the limited source of nerves for transplantation, difficulty in matching tissue structure and size, and long-term denervation of the donor site.

Method used

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  • Artificial nerve graft prepared by electrostatic spinning and preparation method and special device thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Heat the silk in a weak alkali solution (0.1-10% sodium carbonate or 0.1-10% potassium carbonate) at 50-100°C, then wash the treated fibers with distilled water to obtain silk fibroin fibers, and part of the silk prepared above Silk fibroin fiber is dissolved in the ternary system of calcium chloride, ethanol and water at 25-80°C (eg 25°C, 50°C, 80°C) (the molar ratio is calcium chloride: ethanol: water = 1: 2:8), the dissolution time is 0.5 to 6 hours (for example 0.5, 3, and 6 hours), the dissolved mixed solution is packed into a cellulose membrane bag, and dialyzed with distilled water. Then inject the dialyzed silk fibroin solution into a flat mold, and obtain a silk fibroin film after drying, and dissolve the obtained silk fibroin film with formic acid to form a spinning stock solution with a solution concentration of 13% (mass concentration), and then use electrospinning The process uses the above-mentioned special device for preparing the artificial nerve graft p...

Embodiment 2

[0023] Chitosan is dissolved with weak acid, and weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), and concentration is 2~15% (example 2%, 8%, 15%), adds the collagen solution of certain concentration (example 5%, 25%, 50%) to prepare a spinning stock solution with a concentration (mass concentration) of 10%, and then use the electrospinning process to carry out molding processing with the above-mentioned special device for preparing the artificial nerve graft prepared by electrospinning, and the micrometering pump After metering, the spinning stock solution enters the solution injector, sprays the spinning solution on the collecting roller that rotates and moves back and forth to form nanofibers, and forms a tube on the collecting roller. The injection speed is 0.2ml / h, the distance between the end of the solution injector and the collecting roller is 8-11cm, the translation speed of the collecting roller is 2m / hour, and the rotating speed is 90 rpm. Th...

Embodiment 3

[0025] Dissolve polyglycolic acid (PGA), polylactic acid (PLA), or glycolic acid lactic acid copolymer (PLGA, 50 / 50) in chloroform into a spinning solution with a solution concentration of 10-20% (mass concentration), and then use electrospinning The silk process uses the above-mentioned special device for preparing the artificial nerve graft prepared by electrospinning to carry out molding processing. The spinning stock solution is metered by a micro metering pump and then enters the solution injector, and the spinning solution is sprayed on the collecting roller that rotates and moves back and forth. , forming nanofibers, and forming a tube on the collection roller, the voltage of the high-voltage electrostatic generator during the above-mentioned forming process is 20-30KV, the solution injection speed is 0.2ml / h, and the distance between the end of the solution injector and the collection roller is 7-11cm, The translational speed of the collecting roller is 2m / hour, and the...

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Abstract

The invention discloses an artificial nerve graft prepared by electrostatic spinning and a preparation method and a special device thereof, and a product is a catheter-shaped nerve graft which is prepared from polymers by an electrostatic spinning method and consists of nano-fibers. The used materials of the product are all degradable materials and have good biocompatibility with human bodies. Theprepared product does not contain heterotoxin or side-effect substances introduced by a preparation process. A three dimensional structure with plentiful micropores is arranged on a conduit wall, provides a necessary approach for transporting nutritive substances needed in the growth process of nerve cells, has good use effect and provides a necessary induction function and a necessary growing space for the growth of the nerve cells.

Description

Technical field: [0001] The invention relates to an artificial nerve graft for bridging damaged peripheral nerves and promoting their regeneration and repair and a preparation method thereof. Background technique: [0002] With the process of social modernization and the acceleration of people's life rhythm, more traffic accidents, industrial accidents, sports accidents and other incidents will inevitably occur. At the same time, frequent local wars, violent incidents and natural disasters such as earthquakes will cause peripheral nerve Injury, clinically, but when the long-distance nerve defect cannot rely on end-to-end suture to make up for the nerve loss, it has to rely on grafts to bridge the repair. Although it has been more than 100 years to find and develop more suitable grafts, in addition to autologous nerves being the preferred bridge grafts for nerve defects, humans are still looking for ideal implants that can be widely used clinically. Nerve graft substitutes. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61B17/00A61L27/26A61L27/24A61L31/06
CPCD01F6/625D01F9/00A61L27/28A61L27/24A61L2400/12A61L27/18A61L27/227A61L27/58D01D5/0076D01F4/02A61L2430/32A61L27/56C08L5/08C08L67/04A61F2/02A61L27/222A61L27/26D01D5/00D01D5/003
Inventor 杨宇民顾晓松丁斐徐山青严晓莉
Owner NANTONG UNIVERSITY
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