Experiment method for studying effect of tenuigenin on synaptic transmission of rat hippocampal neuron
A hippocampal neuron and polygala saponin technology, which is applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problem that neurological drug experiments cannot achieve the desired effect, and the pharmacological mechanism of neurological drugs cannot be clearly explored. It can meet the needs of neuropharmacological research and other issues
Inactive Publication Date: 2014-11-05
JIANGXI SCI & TECH NORMAL UNIV
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TEN can protect neurons by inhibiting the secretion of Aβ protein. In our previous work, we also found that TEN can significantly improve the learning and memory ability of animals, and can protect cultured neurons against H 2 o 2 The hippocampal structure of the brain is an important brain area closely related to neural functions such as learning and memory in the brain center. Therefore, changes in synaptic transmission of neurons in the hippocampus have an important impact on neural functions such as learning and memory. However, so far So far, there is still no direct evidence that TEN has a regulatory effect on neuronal synaptic transmission
[0004] Existing drug testing methods cannot achieve ideal results in testing the principle of neurological drugs on nerve cells
The research on the pharmacological mechanism of specific neurological drugs cannot be fully explored
Therefore, the pharmacological research of neurological drugs cannot be satisfied
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Embodiment 1
[0096] The technical scheme adopted in the present invention is a test method for the effect of polygala saponins on the synaptic transmission of rat hippocampal neurons, and the medicines and reagents used in the test method:
[0097] Artificial cerebrospinal fluid, namely ACSF, its components are: NaCl 124 mmol / L, KCl 2.5 mmol / L, NaHCO 3 26 mmol / L, NaH 2 PO 4 1.25 mmol / L, CaCl 2 2. 0 mmol / L, MgSO 4 1.3 mmol / L, D-glucose 10 mmol / L, pH 7.4;
[0098] The electrode inner solution for recording spontaneous postsynaptic current, its composition is: KCl 140 mmol / L, NaCl 6 mmol / L, HEPES 10 mmol / L, EGTA 10 mmol / L, Mg-ATP 2 mmol / L, NaGTP is 0.1 mmol / L, pH value is 7.2;
[0099] Electrode inner solution for recording spontaneous excitatory postsynaptic current, its components are: K gluconate 140 mmol / L, NaCl 6 mmol / L, HEPES 10 mmol / L, EGTA 0.2 mmol / L, Mg-ATP 2 mmol / L, Na-GTP 0.1 mmol / L, pH 7.2;
[0100] The electrode inner solution for recording spontaneous inhibitory pos...
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Abstract
The invention discloses an experiment method for studying the effect of tenuigenin on synaptic transmission of rat hippocampal neuron. The method adopts a micro control instrument, a microscopic, and a recording electrode to monitor the spontaneous current state of neuron in tenuigenin with different concentrations, and then the monitor current data is analyzed so as to obtain the influence mechanism and effect of tenuigenin on rat hippocampal neuron. The method adopts a whole-cell patch-clamp technology to research the effect of tenuigenin on spontaneous postsynaptic current in the rat brain hippocampal CA1 area so as to disclose the regulating effect and mechanism of tenuigenin on neuron synaptic transmission, thus the requirements of scientific research and drug development are fulfilled, and at the same time a basis is provided for the further research on the signal transmission mechanism of neuron. The method can be widely used in the pharmaceutical theory researches on neuron, the experiment method and equipment design are scientific and reasonable, and requirements of scientific research can be met.
Description
[0001] technical field [0002] The invention relates to a test method for the effect of drugs on neurons, in particular to a test method for the effect of polygala saponin on synaptic transmission of rat hippocampal neurons. Background technique [0003] Polygalae (Radices polygalae) is the dried root of Polygala tenuifolia Willd., a plant of the genus Polygala in the family Polygala. In traditional Chinese medicine, it has always been regarded as having the effect of calming the nerves and improving intelligence. Therefore, the research on brain function has received extensive attention. Polygala tenuifolia Saponin (tenuigenin, TEN) is a class of main active ingredients isolated from Polygala, which has a wide range of biological activities in anti-aging, intelligence development, anti-oxidation, etc. TEN can protect neurons and improve cognitive functions, etc. aspect has a promoting effect. TEN can protect neurons by inhibiting the secretion of Aβ protein. In our previo...
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IPC IPC(8): G01N27/00
Inventor 姚丽华孟玮孙薇常军李玉萍刘新平袁春华刘伟柱陈冲南小华
Owner JIANGXI SCI & TECH NORMAL UNIV
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