Apparatus and method for rapidly assessing nano-material on biological security of breathing system

Abstract

The invention relates to an apparatus and a method for rapidly assessing a nano-material on biological security of a breathing system, the apparatus comprises a glass microprobe, an Ag/AgCl electrode placed in the glass microprobe, a reference Ag/AgCl electrode, a culture dish with cells and cell culture fluid, a patch clamp preposed current power amplifier, a patch clamp digital-analog/analog-to-digital converter, a SICM negative feedback scanning control circuit and a SICM high precision XYZ three dimensional piezoelectricity ceramic scanner. The method comprises the following steps: using a SICM technology for detecting the surface three dimensional structure of an in-vivo biological sample under the physiological liquid state culture condition, the change of microscopic appearance of cell surface before adding nano-material on the vivo-cell and after adding nano-material on the vivo-cell can be observed; influence of nano-material on vivo-cell function can be researched, a traditional patch clamp technology is employed, the biological security of the nano-material can be assessed by recording the voltaic change of the ion channels before adding the nano-material and after adding the nano-material. The apparatus and method of the invention have the advantages of easy realization and rapidity by using combination of SICM and the patch clamp technology, the security of the nano-material can be assessed from the structure and function.

Description

(1) Technical field: [0001] The present invention belongs to the field of evaluating the safety of nanomaterials, and in particular refers to a technical method for evaluating the safety of nanomaterials by using changes in cell surface microtopography and electrophysiological recording results, that is, a device for rapidly evaluating the biological safety of nanomaterials on the respiratory system and method. (two) background technology: [0002] Nanomaterials are very likely to enter the atmosphere during their production, transportation, use, and disposal. After being absorbed by the human body through the respiratory tract, they are likely to cause serious damage to human health and become the cause of many major diseases. Concerns about the biosafety of nanomaterials. Commonly used nanomaterials include nano ZnO, TiO 2 , C 60 , CuO, etc., which have been widely used in many aspects related to human life, such as quantum devices, special fine ceramics, solar energy u...

AI Technical Summary

Problems solved by technology

[0005] The current detection methods for cell structure and function have the following deficiencies: Although the scanning electron microscope has a high resolution, it cannot perform three-dimensional imaging of non-conductive living biological samples due to its requirements on the conductivity of the sample. It is achieved by a special sample preparation method of sputtering or sputtering conductive (platinum) material on the sample surface, which is time-consuming and laborious, and the sample preparation is cumbersome and complicated; the atomic force microscope uses the interaction force between the needle tip and the sample to control the negative feedback of the needle tip on the sample surface. The scanning imaging of the biological sample will not only produce more or less force on the surface of the biological sample, but also make it difficult to image the soft biological sample, especially the high-resolution imaging of the alveolar epithelial microvilli cannot be obtained; although ordinary patch clamp technology can evaluate The physiological function of the cell, but because it only uses an ordinary optical microscope for probe positioning, the resolution is limited by the optical diffraction limit, and it is difficult to link the changes in the physiological function of the cell with the high-resolution membrane structure

In addition, the evaluation of the safety of most nanomaterials requires animal experiments, which are not only costly and time-consuming, but also have large differences among individual animals, resulting in poor reliability and repeatability of the results.

Images