Process of controlling monochain nucleic acid punching speed by optical nickle

Abstract

The present invention relates to biotechnology, and is especially optical forceps method of controlling the hole passing rate of single chain nucleic acid. The method includes the following steps: 1. capturing single chain DNA or RNA connected magnetic bead with optical forceps in the negative pole of electrophoresis tank; 2. separating the two poles of the electrophoresis tank with film possessing single nanometer hole so as to perform ion exchange only through the nanometer hole; and 3. turning on the power source for the current to draw the free end of the DNA towards the positive pole, controlling the moving speed of the optical forceps in 0.5-1 base / ms to control the motion of DNA, recording the electric signal during penetrating the nanometer hole with the patch clamp and converting the electric signal into sequence information in the computer. The present invention is superior to available technology, which has too fast nucleic acid speed for patch clamp to recognize.

Description

technical field

[0001] The invention relates to a method in the technical field of bioengineering, in particular to a method for controlling the perforation speed of single-stranded nucleic acid by optical tweezers. Background technique

[0002] With the advent of the post-genome era, the demand for genome sequencing is increasing dramatically, but the current sequencing methods are slow, expensive, and have gaps. In fact, before the start of the Human Genome Project, some people began to explore new methods of DNA sequencing. In particular, The National Institutes of Health in the United States launched the $1000 Genome in 2004 (that is, in the next 10 years or so, the cost of sequencing mammals Since it was reduced to about $1000, the speed was increased by 3-4 orders of magnitude, the error rate was within 1 / 10,000, and there was basically no gap), many new sequencing ideas have emerged. Among them, the use of patch clamp to detect single-stranded DNA (referred to as Sin...