Electromagnetic method and device for controlling single-chain nucleic acid perforating speed

Abstract

The present invention relates to bioengineering technology, and is electromagnetic method and equipment for controlling single-chain nucleic acid perforation. The method includes the following steps: 1. fixing the composition of one single-chain target DNA or RNA and one connected magnetic bead onto the negative pole of electrophoresis tank with two nanometer hole separated poles by using one electromagnet; and 2. turning on the power source to apply the free end of the negatively charged target molecule with one pull force towards the positive pole, trimming the electromagnetic force to release the magnetic bead while maintaining certain attraction of the electromagnet on the magnetic bead so as to make the target molecule to move slowly towards the positive pole and control the nanometer hole passing speed in 0.5-1 base / ms, recording the signal with the patch clamp and converting the signal into sequence information in the computer. The equipment includes electrophoresis tank, nanometer element, computer, etc.

Description

technical field

[0001] The present invention relates to a method in the technical field of bioengineering, in particular to a method and device for controlling the perforation speed of single-stranded nucleic acid by electromagnetic force. Background technique

[0002] With the advent of the post-genome era, the demand for genome sequencing is increasing dramatically, but the current sequencing methods are slow, expensive, and have gaps. In fact, before the launch of the Human Genome Project, some people began to explore new methods of DNA sequencing. In particular, The National Institutes of Health in the United States launched $1000 Genome in 2004 (that is, in the next 10 years or so, the cost of mammalian sequencing will be reduced. To about $1000, the speed is increased by 3-4 orders of magnitude, the error rate is within 1 / 10,000, and there is basically no gap), and many new sequencing ideas have emerged. Among them, the use of patch clamp to detect single-stranded DNA ...

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