Application of astragalus extract containing astragalus polysaccharide in promoting plant growth

Abstract

The invention belongs to the technical field of agriculture, and particularly relates to an application of an astragalus extract containing astragalus polysaccharide in promoting plant growth. Purified astragalus polysaccharide is proved to have good growth regulation and stress resistance enhancement on plants, but the extraction and purification process of polysaccharide is high in cost, low in efficiency and difficult to meet the requirements of large-scale production, and the application in agriculture is limited. Provided in the present invention is the application of the astragalus extract containing astragalus polysaccharide, in particular the application of same as a plant growth promoter in promoting plant growth and enhancing plant stress resistance. The use concentration of astragalus polysaccharide is reduced, the application of astragalus polysaccharide in agriculture is promoted, and a new option for regulating plant growth in agriculture is provided.

Description

Application of astragalus extract containing astragalus polysaccharide in promoting plant growth Technical Field

[0001] The priority information of this invention is as follows: Priority number 202311488571X, priority date November 9, 2023, invention name: Use of Astragalus Polysaccharides in Promoting Plant Growth, the disclosure of which is incorporated herein by reference. The present invention belongs to the field of agricultural technology and specifically relates to the use of an Astragalus extract containing Astragalus polysaccharides in promoting plant growth. Background Art

[0002] Astragalus membranaceus (Huangqi) boasts a long history and extensive clinical applications, making it one of the most commonly used Chinese medicines throughout the ages. Its use as a medicine was first recorded in Shennong's Herbal Classic, where its original name, Huangqi (Huangqi), was listed as a top-grade herb. It has been documented in numerous subsequent herbal compendiums. It boasts benefits such as tonifying Qi and strengthening the exterior, promoting diuresis and expelling toxins, draining pus, and healing sores and promoting tissue regeneration. Westerners began to recognize the medicinal value of Astragalus membranaceus in the 19th century, with the first report of its use by Dr. Alexander Von Bange, a physician specializing in East Asian plants, in 1868. In his 1911 book, The Raw Materials of China, G.A. Stuart noted that Astragalus membranaceus "has a good reputation as a tonic, cough suppressant, and diuretic."

[0003] Astragalus polysaccharides have a wide range of biological activities, and their research has mainly focused on the fields of traditional Chinese medicine, animal husbandry and agriculture. In the mid-1980s, An Jinhua, Karen S. Liu, Edwin S. Linax, John H. Muser and others from Pan-China Corporation, a world-renowned botanical medicine research and development institution, spent 10 years screening out astragalus polysaccharides purified from the higher plant Astragalus from nearly 30 Chinese herbal medicines in the Chinese pharmacopoeia. They have the ability to comprehensively and continuously increase whole blood cells, stimulate the hematopoietic system, and enhance the body's immunity. Later studies found that purified astragalus polysaccharides can promote plant growth and development and improve the plant's own resistance. For example, in the paper "Research on the Extraction of Astragalus Polysaccharides and Their Effect on Improving Plant Resistance" published by Qiu Benjun, it was found that 10 mg / mL (10,000 ppm) of purified astragalus polysaccharides (purity above 90%) can increase the germination rate of seeds, 1 mg / mL (1,000 ppm) of purified astragalus polysaccharides can promote the growth of seedling roots, 0.1 mg / mL (100 ppm) of purified astragalus polysaccharides can increase the chlorophyll content of plant leaves, and 0.1-1 mg / mL (100-1,000 ppm) of purified astragalus polysaccharides can enhance the plant's resistance to stress.

[0004] Astragalus polysaccharides (APS) have a wide range of pharmacological effects and are valuable immunopotentiators. They have significant protective effects on target organs and can counteract the adverse effects of radiotherapy and chemotherapy. Furthermore, APS can comprehensively increase whole blood cell counts, regulate immune function, and have anti-tumor effects, making them widely used in medicine. In agriculture, purified APS have been shown to effectively regulate plant growth and enhance stress resistance. However, the extraction and purification process requires highly specialized techniques and equipment, resulting in high extraction costs and low efficiency, making it difficult to meet the needs of large-scale production, limiting its application in agriculture.

[0005] Summary of the Invention

[0006] Astragalus extracts are rich in astragalus polysaccharides, which are their active ingredient. They also contain at least one active ingredient selected from saponins, flavonoids, amino acids, alkaloids, monosaccharides, proteins, and trace elements. The present invention has found that astragalus extracts can significantly promote plant growth at low polysaccharide concentrations and also increase the plant's resistance to stress. Compared with the prior art, the present invention has a simpler extraction process and lower costs. The synergistic effect between the various components in the extract can greatly reduce the concentration of astragalus polysaccharides used, promoting their application and providing a new option for regulating plant growth in agriculture.

[0007] The present invention provides application of an astragalus extract containing astragalus polysaccharide in promoting plant growth.

[0008] An astragalus extract for promoting plant growth comprises at least one of an astragalus water extract, an astragalus water-extracted alcohol precipitate, and an astragalus extract with an ethanol content greater than 0 and less than or equal to 60%. The astragalus extract contains astragalus polysaccharide as a marker component, and its concentration is effective within the range of 0.01-1000 ppm.

[0009] The astragalus extract includes astragalus polysaccharide and at least one saponin, flavonoids, amino acids, alkaloids, monosaccharides, proteins, and trace elements; preferably, the astragalus water extract includes astragalus polysaccharide, monosaccharide, protein, amino acids, Fe, Cu, Zn, K, and Ca as active ingredients.

[0010] In the present invention, promoting plant growth includes promoting plant germination, promoting the growth of one or more of roots, stems, leaves, or flowers and fruits. For example, promoting the growth of root length, stem diameter, plant height, leaf width, leaf length, leaf number, leaf area, biomass, chlorophyll content, yield, etc.

[0011] In the present invention, the astragalus extract is used as an active ingredient of the growth promoter and can be used alone or in combination with other products.

[0012] The present invention provides a plant growth promoter. The astragalus extract comprises at least one of an astragalus water extract, an astragalus water-extracted alcohol precipitate, and an astragalus extract with an ethanol content of greater than 0 and less than or equal to 60%. The astragalus extract uses astragalus polysaccharide as a marker component and is effective within a concentration range of 0.01-1000 ppm.

[0013] The "growth promoter" mentioned in the present invention refers to a plant growth promoter, which is a biologically active substance that can promote cell division, differentiation and elongation growth, or promote the growth of plant vegetative organs and the development of reproductive organs.

[0014] In the present invention, the astragalus extract containing astragalus polysaccharide is a natural plant growth promoter.

[0015] The plants described in the present invention include but are not limited to economic crops and food crops, such as tobacco, corn, lettuce, wheat, pepper, Chinese cabbage, lettuce, bok choy, tomato, citrus, kiwi, cherry, pear, apple, cotton, cucumber, eggplant, etc.

[0016] The so-called "economic crops" are of various types, including but not limited to fiber crops (such as cotton, hemp, etc.), oil crops (such as sesame, peanuts, etc.), sugar crops (such as sugarcane, sugar beets, etc.), hobby crops (tobacco), medicinal crops, dye crops, ornamental crops, fruits (such as citrus, kiwi, cherry, pear, apple, etc.) and other economic crops (such as lettuce, pepper, Chinese cabbage, lettuce, Shanghai green, etc.).

[0017] The “food crops” mentioned above include but are not limited to cereal crops (wheat, rice, corn), tuber crops (including sweet potatoes, potatoes, etc.) and legume crops (including soybeans, broad beans, peas, mung beans, etc.).

[0018] In the product of the present invention, the Astragalus extract can be used directly as a single dose. In order to make it stable and easy to transport and store, it can be made into an agricultural product, for example, by adding auxiliary materials to make a corresponding dosage form. The auxiliary materials can be conventional auxiliary materials in the field, such as dispersants, wetting agents, binders, emulsifiers, stabilizers, solvents, etc.

[0019] On the other hand, the astragalus extract of the present invention can be used as a synergist in combination with foliar fertilizers, water-soluble fertilizers, compound fertilizers, pesticides and other products.

[0020] In the present invention, the dosage form of the agricultural product includes but is not limited to emulsifiable concentrate, suspension concentrate, wettable powder, dust, granule, aqueous solution, mother liquor or mother powder.

[0021] In the present invention, when used, the single agent or agricultural product prepared from the astragalus extract is used to treat seeds, spray on leaves or irrigate roots.

[0022] In the present invention, when the astragalus extract or its product is prepared into a solution for promoting seed germination or plant growth, the concentration of astragalus polysaccharide in the solution can be selected according to actual needs.

[0023] For example, it can be selected from 0.01-2000ppm, 0.01-1000ppm, 0.01-500ppm, or 0.01ppm, 0.02ppm, 0.03ppm, 0.1ppm, 0.2ppm, 0.3ppm, 1.0ppm, 2.0ppm, 3.0ppm, 10ppm, 20ppm, 30ppm and the like.

[0024] The seeds are treated by mixing a single agent or agricultural product made from the astragalus extract with the seeds to coat the seeds with the extract, or soaking the seeds to be germinated with the extract solution.

[0025] In the growth promotion experiment of the present invention, the influence of diseases and insect pests on plant growth has been eliminated. Through such an experimental design, the results are sufficient to show that the effect of Astragalus extract on promoting plant growth is not caused by avoiding diseases and insect pests.

[0026] The present invention also provides an application of an astragalus extract containing astragalus polysaccharide in regulating plant stress resistance. The astragalus extract includes at least one of an astragalus water extract, an astragalus water-extracted alcohol precipitate, and an astragalus extract with an ethanol content greater than 0 and less than or equal to 60%. The astragalus extract contains astragalus polysaccharide as the active ingredient, and the concentration is effective within the range of 0.01-1000ppm.

[0027] Plant stress resistance refers to certain traits that plants possess to resist adverse environments, such as cold resistance, drought resistance, salt resistance, high temperature resistance, waterlogging resistance, and UV damage resistance.

[0028] In the present invention, the astragalus extract containing astragalus polysaccharide regulates the stress resistance of plants, including at least one of salt resistance, cold resistance, drought resistance, high temperature resistance, waterlogging resistance, and UV damage resistance.

[0029] Beneficial effects of the present invention: Compared with the existing technology, the present invention has a simple extraction process and low cost. The synergistic effect between the components in the extract can greatly reduce the usage concentration of astragalus polysaccharide, promote the application of astragalus polysaccharide, and also provide a new option for regulating plant growth in agriculture.

[0030] The content of astragalus polysaccharide in the astragalus extract applicable to the present invention can be selected from 10% to 50%. DETAILED DESCRIPTION

[0031] The following is a clear and complete description of the technical solution of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0032] Seed germination rate = (number of germinated seeds / number of test seeds) × 100%;

[0033] Seed germination potential = (number of seeds germinated at the peak of germination / number of test seeds) × 100%;

[0034] Root length: The distance from the rhizome junction to the root tip is the root length of this plant;

[0035] Plant height: the distance from the base of the plant to the tip of the topmost expanded leaf (i.e. the total length of the plant).

[0036] Example 1 Preparation of Astragalus Extract

[0037] Wash and slice the Astragalus, place it in a forced air drying oven to dry, and then grind it into fine powder using a grinder for later use.

[0038] 1) Preparation of Astragalus Water Extract and Alcohol Precipitate: Weigh a predetermined amount of Astragalus and add 10 times the volume of water. Extract with stirring at 50°C for 120 minutes. Repeat the above steps, combine the two extraction filtrates, centrifuge at 4000 rpm for 15 minutes, collect the supernatant, pass it through a 100 kDa ceramic membrane, and concentrate it at 55°C to 1 / 4 of its original volume. Add 5 times the volume of anhydrous ethanol and precipitate it at 4°C overnight. Filter the precipitate, and freeze-dry it to obtain an Astragalus Polysaccharide Water Extract and Alcohol Precipitate. The polysaccharide concentration was determined to be 50%.

[0039] 2) Preparation of Astragalus Aqueous Extract: A predetermined amount of Astragalus was weighed and 10 times the volume of water was added. The mixture was stirred and extracted at 50°C for 120 minutes. The above process was repeated. The two extraction filtrates were combined and centrifuged at 4000 rpm for 15 minutes. The supernatant was collected and passed through a 100 kDa ceramic membrane. Rotary concentration was performed at 55°C to a solids content of 40-50%. The polysaccharide content was determined to be 30%.

[0040] 3) Preparation of 20% ethanol Astragalus extract: Weigh a certain amount of Astragalus and add 10 volumes of 20% (v / v) ethanol. Extract with stirring at 50°C for 120 minutes. Repeat the above steps, combine the two extraction filtrates, centrifuge at 4000 rpm for 15 minutes, remove the supernatant, pass it through a 100 kDa ceramic membrane, and concentrate it at 55°C with a rotary evaporation to a solid content of 40-50%. The polysaccharide content was determined to be 25%.

[0041] 4) Preparation of 40% ethanol Astragalus extract: Weigh a certain amount of Astragalus and add 10 volumes of 40% (v / v) ethanol. Stir and extract at 50°C for 120 minutes. Repeat the above steps. Combine the two extraction filtrates and centrifuge at 4000 rpm for 15 minutes. The supernatant is passed through a 100 kDa ceramic membrane and rotary concentrated at 55°C to a solid content of 40-50%. The polysaccharide content is determined to be 18%.

[0042] 5) Preparation of 60% ethanol Astragalus extract: Weigh a certain amount of Astragalus and add 10 volumes of 40% (v / v) ethanol. Extract with stirring at 50°C for 120 minutes. Repeat this process, combine the two extraction filtrates, centrifuge at 4000 rpm for 15 minutes, remove the supernatant, pass it through a 100 kDa ceramic membrane, and concentrate it at 55°C with a rotary evaporation to a solid content of 40-50%. The polysaccharide content was determined to be 10%.

[0043] Example 2 Experiment on promoting growth of rice seeds by soaking different astragalus extracts

[0044] 1. Experimental Design

[0045] The experimental samples were the different Astragalus extracts in Example 1, which were prepared with water to form a mother liquor with a polysaccharide concentration of 1% (i.e., 10,000 ppm). After the agent was diluted with water according to the concentration designed in Table 1, rice seeds were soaked to test the growth-promoting effects of different Astragalus extracts on rice.

[0046] Table 1 Experimental design

[0047] 2. Experimental methods

[0048] Rice seeds were disinfected with 5% sodium hypochlorite for 15 minutes, rinsed 6-7 times with clean water, and dried with filter paper. Astragalus polysaccharide (APS) was then prepared with water to a concentration of 0.01-100 ppm according to the experimental design. The seeds were soaked in various concentrations of APS, covering the seeds completely. The seeds were then soaked at 25°C for 16 hours. The seeds were then rinsed 3-4 times with clean water to remove the surface APS. The seeds were then dried and set aside. The soaked seeds were sown in disposable square Petri dishes filled with planting soil, with 36 seeds per dish. Each treatment was replicated at least three times. Full, intact seeds were selected for sowing. Each seed was pressed to the bottom of the dish, then gently pressed and smoothed. The dish was covered and incubated in a 25°C incubator under a light intensity of 10,000 lux and a 14-hour light cycle with 10 hours of darkness. After three days, the number of germinations in each treatment group was assessed and germination potential was calculated. Seven days later, the aboveground portion of the seedlings was excised and measured for shoot length.

[0049] 3. Experimental results

[0050] The experimental results are shown in Table 2. From the experimental results, it can be seen that different astragalus extracts have different degrees of growth-promoting effects on rice seed soaking at concentrations of 0.1-100ppm. Among them, the optimal growth-promoting concentration of astragalus water extract, astragalus water extract alcohol precipitate, 20% ethanol astragalus extract and 40% ethanol astragalus extract is 1ppm, and the optimal growth-promoting concentration of 60% ethanol astragalus extract is 10ppm. There are differences in the growth-promoting effects of each extract. From the perspective of rice germination potential and sprout length indicators, the order of effect strength is astragalus water extract > 20% ethanol astragalus extract > 40% ethanol astragalus extract > astragalus water extract alcohol precipitate > 60% ethanol astragalus extract.

[0051] Table 2 Results of promoting rice growth by soaking seeds with different Astragalus extracts

[0052] Example 3 Experiment on the Effect of Different Astragalus Extracts on the Low Temperature Resistance of Cucumber

[0053] 1. Experimental Design

[0054] The experimental samples were the different Astragalus extracts in Example 1, which were prepared with water to form a mother liquor with a polysaccharide concentration of 1% (i.e., 10,000 ppm). After the agent was diluted with water according to the concentration designed in Table 3, the cucumbers were sprayed with leaves to test the anti-low temperature effects of different Astragalus extracts on cucumbers.

[0055] Table 3 Experimental design

[0056] 2. Experimental methods

[0057] Select cucumber seedlings with consistent growth, prepare different concentrations of pesticides, and spray the cucumber seedlings on the leaves. Control the number of sprays per pot so that the liquid is evenly distributed on the leaf surface but does not drip, to ensure that the spray amount of each treatment is consistent. Set up 6 pots of seedlings for each treatment. After spraying, each treatment continues to be cultured in a 25℃ culture room. After 24 hours of seedling acclimatization, place them in a 4℃ low-temperature chamber overnight for low temperature stress. After 16 hours, the leaves of each treatment begin to wilt due to water loss. Cut off the first true leaf of each seedling to measure the leaf fresh weight. After each leaf fully absorbs water in water for 24 hours, absorb the surface moisture, measure the saturated fresh weight of the leaf again, and calculate the leaf water loss rate.

[0058] 4. Experimental results

[0059] The experimental results are shown in Table 4. From the experimental results, it can be seen that different Astragalus extracts at a concentration of 0.1-100ppm can be sprayed on the leaves of cucumbers to varying degrees to improve the low temperature resistance of cucumber seedlings, but there are differences in the effects. From the experimental results, it can be seen that the order of the effects of each extract is: Astragalus water extract > 20% ethanol Astragalus extract > 40% ethanol Astragalus extract > Astragalus water extract alcohol precipitation > 60% ethanol Astragalus extract, among which Astragalus water extract has the best effect. At the optimal concentration of 1ppm, the water loss rate of cucumber leaves is 18.11%, which is significantly lower than the water loss rate of leaves in the CK treatment group (31.21%), indicating that Astragalus water extract can significantly improve the low temperature resistance of cucumber seedlings.

[0060] Table 4 Effects of different Astragalus extracts on low temperature resistance of cucumber

[0061] Example 4 Experiment on promoting tomato seed germination with Astragalus water extract and pure Astragalus polysaccharide

[0062] Examples 2 and 3 show that, among the various Astragalus extracts, the aqueous Astragalus extract outperformed the other extracts in promoting growth and resisting stress. Previous studies have reported that Astragalus polysaccharide is the primary component of Astragalus, and two purified Astragalus polysaccharide products with purities exceeding 90% have been obtained through isolation and purification. Furthermore, Astragalus contains a variety of active ingredients, including amino acids, proteins, monosaccharides, and trace elements beneficial to plant growth. This example compared the growth-promoting activity of the aqueous Astragalus extract and the purified Astragalus polysaccharide in a tomato seed germination experiment.

[0063] 1. Experimental samples

[0064] Experimental Sample: The Astragalus water extract was obtained from Example 1. The results of the analysis of the various components are shown in Table 5. The polysaccharide contents of purified Astragalus polysaccharide A and purified Astragalus polysaccharide B were 92% and 95%, respectively. The preparation methods were based on the paper "Study on the Extraction of Astragalus Polysaccharides and Their Effect on Improving Plant Resistance" published by Qiu Benjun.

[0065] Table 5 Determination results of various components in Astragalus membranaceus water extract

[0066] 2. Experimental Design

[0067] The experimental sample was prepared with water to form a mother liquor with a polysaccharide concentration of 1% (i.e., 10,000 ppm). After diluting the agent with water according to the concentration designed in Table 6, tomato seeds were soaked to test the germination-promoting effect of the astragalus water extract and the purified astragalus polysaccharide on tomato seeds.

[0068] Table 6 Experimental design

[0069] 3. Experimental methods

[0070] Select plump and uniform-sized tomato seeds. According to the experimental design in Table 6, soak the seeds for 4 h in water (CK), 0.1, 1, 10, 100, 1000, and 10,000 ppm of the agent, respectively. Wash the agent on the surface of the seeds with water and place them in germination boxes padded with two filter papers. Place 60 seeds in each germination box to ensure uniform distribution of seeds. Set up 3 replicates for each treatment and place them in a culture room at 25°C. Record the number of seeds germinated every day, and calculate the germination potential after 3 days and the germination rate after 7 days.

[0071] 3. Experimental results

[0072] The experimental results are shown in Table 7. The germination potential and germination rate of tomatoes in the CK control group were 78.18% and 91.21%, respectively. Treatment with the Astragalus water extract significantly promoted tomato seed germination at concentrations ranging from 0.1 to 100 ppm, with the optimal concentration being 1 ppm. At this concentration, the germination potential and germination rate reached 87.78% and 99.14%, respectively, significantly higher than those of the CK control. When the polysaccharide concentration reached 10,000 ppm, it inhibited tomato seed germination. Both purified Astragalus polysaccharides A and B showed an increasing promotional effect with increasing polysaccharide concentration. While the promotion effect was not significant at low concentrations of 0.1 to 10 ppm, it was significant at high concentrations of 100 to 10,000 ppm. The optimal effect was achieved when the purified polysaccharide concentration reached 10,000 ppm. These results are consistent with those reported in the paper "Study on the Extraction of Astragalus Polysaccharides and Their Effect on Improving Plant Resistance." From the above results, it can be seen that the Astragalus extract of the present invention has a significant effect on promoting tomato seed germination within a relatively low polysaccharide concentration range (0.1-100 ppm). This may be because the Astragalus extract contains other active ingredients in addition to polysaccharides. The synergistic effect between the various components greatly reduces the effective concentration of polysaccharides, making the Astragalus water extract show a significant growth-promoting effect.

[0073] Table 7 Results of promoting tomato seed germination by Astragalus water extract and Astragalus polysaccharide purification

[0074] Example 5 Experiment on drought resistance of wheat using Astragalus membranaceus aqueous extract and pure polysaccharide

[0075] The experimental samples were the same as those in Example 4. The experimental samples were prepared with water to form a mother liquor with a polysaccharide concentration of 1% (i.e., 10,000 ppm). After diluting the agent with water according to the concentration designed in Table 8, wheat seeds were soaked to test the drought resistance effect of the astragalus water extract and the purified astragalus polysaccharide on wheat.

[0076] Table 8 Experimental design

[0077] 3. Experimental methods

[0078] Select plump, uniformly sized wheat seeds and soak them for 16 hours in water (CK), 0.1, 1, 10, 100, 1000, and 10,000 ppm of a pesticide. The surface of the seeds was then cleaned with water for later use. Two sheets of filter paper were placed in a germination box, and 15 mL of a 17% (m / v) PEG6000 solution was added to the filter paper to simulate drought conditions. The treatment group with 15 mL of water served as a non-stress control. Thirty seeds were placed in each germination box, evenly distributed. Three replicates were set for each treatment, and the seeds were cultured in a 25°C incubator. After 7 days, the number of germinated seeds in each treatment group was assessed, the germination rate was calculated, and the root and shoot lengths of the wheat were measured.

[0079] 4. Experimental results

[0080] The experimental results are shown in Table 9. Under non-stress conditions, the germination rate of wheat seeds reached 99.39%, with root lengths of 11.23 cm and shoot lengths of 12.39 cm. Under PEG6000-simulated drought conditions, the germination rate reached 58.18%, with root lengths of 7.01 cm and shoot lengths of 8.26 cm. Both wheat growth and germination were significantly stressed. Treatment with an aqueous Astragalus extract and purified Astragalus polysaccharide improved wheat's drought resistance. Similar to the results of the tomato seed germination promotion assay, the Astragalus extract significantly improved wheat drought resistance at concentrations ranging from 0.1 to 100 ppm. The optimal concentration was 1 ppm, at which the germination rate reached 78.14%, with root lengths of 8.93 cm and shoot lengths of 9.78 cm, significantly exceeding those of the CK control. The polysaccharide concentration also exhibited an inhibitory effect at 10,000 ppm. Both purified astragalus polysaccharides A and B have a significant promoting effect at a concentration of 10-10000ppm, and the effect is best when the purity of the purified polysaccharide reaches 100ppm. From the above results, it can be seen that at the same polysaccharide content, the astragalus water extract of the present invention has a significant effect of promoting wheat germination under drought conditions within a lower polysaccharide concentration range (0.1-100ppm). The optimal concentration of the astragalus water extract is significantly lower than that of the purified astragalus polysaccharide, and the effect is comparable to that of the purified astragalus polysaccharide, which may be related to the synergistic effect between the active ingredients in the astragalus water extract.

[0081] Table 9 Effects of Astragalus water extract and Astragalus polysaccharide purification on drought resistance of wheat

[0082] Example 6 Trial Preparation of Astragalus Extract

[0083] The formulation includes: Astragalus membranaceus water extract, thickener - xanthan gum, dispersant - alkyl glycoside, defoaming agent and water. The specific formulation is shown in Table 10, and trial production is carried out according to the following steps:

[0084] Table 10 Trial formulation of Astragalus membranaceus aqueous extract

[0085] ① Weigh an appropriate amount of xanthan gum and water and shear until the xanthan gum is completely dissolved and dispersed to form a uniform liquid;

[0086] ② The liquid in ① was stirred at 55°C, and the Astragalus extract was accurately weighed and added to a stirred tank, and stirred at 55°C for 30 minutes until the liquid was homogeneous;

[0087] ③ Weigh the alkyl polyglycoside and add it to the above ② homogeneous liquid, stirring for 3 minutes; then add the defoaming agent and stir for 3 minutes to complete the trial preparation of the formula sample.

[0088] After trial production of each formulation, the samples were subjected to high temperature (54°C) and low temperature (0°C) storage stability tests, and the formulation stability investigation was conducted after the samples were stored in hot and cold conditions for 2 weeks.

[0089] The results showed that Formulation 1 became turbid during hot storage; Formulation 2 exhibited significant stratification without the addition of a dispersant; Formulation 3 produced significant foam during dissolution; and Formulation 4 was a homogeneous, stable liquid with good dispersibility. Formulation 4 was tested, and the parameters and results are shown in Table 12.

[0090] Table 11 Test results of Astragalus multi-water extract preparations

[0091] Example 7 Experiment on promoting the growth of pakchoy by spraying the leaves of the Astragalus membranaceus aqueous extract preparation

[0092] 1. Experimental Design

[0093] The experimental sample was the Astragalus membranaceus aqueous extract preparation (polysaccharide content of 15000 ppm) in Example 6. After the agent was diluted according to the concentration designed in Table 12, it was sprayed on the leaves of Chinese cabbage to test the growth-promoting effect of the Astragalus membranaceus aqueous extract preparation on Chinese cabbage.

[0094] Table 12 Experimental design

[0095] 2. Experimental methods

[0096] Select pakchoy seedlings (two true leaves) with uniform growth, prepare the astragalus extract preparation with water to 0.1-10ppm according to the experimental design, spray the pakchoy leaves so that the liquid is evenly distributed on the leaf surface but does not drip, and place them in a culture room for cultivation at a temperature of 25°C. Each treatment group has 6 seedlings. During the experiment, the water and fertilizer management of each treatment group is consistent. After 7 days, the SPAD value, fresh weight and leaf area of ​​the leaves of different treatment groups are investigated.

[0097] 3. Experimental results

[0098] The experimental results are shown in Table 13. From the experimental results, it can be seen that spraying the Astragalus water extract preparation on the leaves of Chinese cabbage can significantly promote the growth of Chinese cabbage. The promotion effect is significant in the range of 0.1-1ppm, and the optimal concentration is 1ppm. Under this concentration treatment, the SPAD value of Chinese cabbage slices can be significantly improved, the accumulation of chlorophyll, and the increase in the fresh weight and leaf area of ​​Chinese cabbage can be significantly promoted. Compared with the clear water control, the promotion rate of the Astragalus water extract preparation on the fresh weight and leaf area of ​​Chinese cabbage reached 37.62% and 21.87%.

[0099] Table 13 Results of leaf spraying of Astragalus membranaceus aqueous extract to promote growth of Chinese cabbage

[0100] Example 7 Experiment on the effect of Astragalus membranaceus aqueous extract on tomato root irrigation and growth promotion

[0101] 1. Experimental Design

[0102] The experimental sample was the Astragalus membranaceus aqueous extract preparation (polysaccharide content of 15,000 ppm) in Example 6. After the agent was diluted according to the concentration designed in Table 14, the tomato roots were irrigated to test the growth-promoting effect of the Astragalus membranaceus aqueous extract preparation on tomato root irrigation.

[0103] Table 14 Experimental design

[0104] 2. Experimental methods

[0105] Tomato seedlings (4 true leaves) with uniform growth were selected, and the astragalus extract preparation was prepared with water at a concentration of 0.1-10 ppm according to the experimental design. The roots were irrigated from the bottom of the pot, with 100 mL per pot. The pots were placed in a culture room for cultivation at a temperature of 25°C. Each treatment group had 6 pots of seedlings. The water and fertilizer management of each treatment group was consistent during the experiment. After 10 days, the plant weight, root weight and stem diameter of the different treatment groups were investigated.

[0106] 3. Experimental results

[0107] The experimental results are shown in Table 15. From the experimental results, it can be seen that the Astragalus water extract preparation can significantly promote the growth of tomatoes by root irrigation. The promotion effect is significant in the range of 0.1-1ppm. The optimal root irrigation concentration is 1ppm. Under this concentration treatment, the stem diameter of tomatoes can be significantly increased and the accumulation of tomato biomass can be significantly promoted. Compared with the clear water control, the promotion rates of the Astragalus polysaccharide preparation treatment group on tomato stem diameter, plant weight and root weight were 12.83%, 27.29% and 23.13%, respectively.

[0108] Table 15 Results of root irrigation of tomato by Astragalus extract preparation

[0109] Example 8 Field Test on the Effect of Astragalus Root Extract and Organic Compound Fertilizer on Lettuce Growth

[0110] Test crop: Lettuce (variety: Xiayou No. 10)

[0111] 1. Experimental Design

[0112] The experimental sample was the Astragalus membranaceus aqueous extract preparation (polysaccharide content of 15,000 ppm) in Example 6. After the agent was diluted according to the concentration designed in Table 16, the lettuce was irrigated to test the field growth-promoting effect of the Astragalus membranaceus aqueous extract preparation on the lettuce.

[0113] Table 16 Experimental design

[0114] 2. Experimental methods

[0115] The experiment involved three treatment concentrations, each with three replicate plots. Each treatment consisted of 150 plants. Each plant was irrigated with 150 ml of a compound fertilizer (NPK: 24-10-16) as a base fertilizer. Ten days after the root irrigation treatment, 10 plants were randomly selected from each plot to measure plant height, root length, biomass, SPAD value, and leaf number.

[0116] 3. Experimental results

[0117] The experimental results are shown in Table 17. From the experimental results, it can be seen that root irrigation of lettuce using a mixture of an aqueous extract of Astragalus membranaceus and an organic compound fertilizer in the field can significantly promote the growth of lettuce. The promotion effect is significant within the range of 0.1-1ppm, and the optimal concentration is 1ppm. Under this concentration treatment, the SPAD value and the number of leaves of the lettuce seedlings can be significantly increased. Compared with the clear water control, the plant height promotion rate is as high as 23.93%, and the plant weight promotion rate is as high as 19.54%.

[0118] Table 17 Astragalus extract preparations promote the growth of lettuce

[0119] Example 9 Field Test on the Effect of Mixing Astragalus Root Extract and Foliar Fertilizer on Pepper Growth

[0120] Test crop: pepper (variety: Erjingtiao)

[0121] 1. Experimental Design

[0122] The experimental sample was the Astragalus membranaceus aqueous extract preparation (polysaccharide content of 15000 ppm) in Example 6. After the agent was diluted according to the concentration designed in Table 18, it was sprayed on the leaves of peppers to test the field growth-promoting effect of the Astragalus membranaceus aqueous extract preparation on peppers.

[0123] Table 18 Experimental design

[0124] 2. Experimental methods

[0125] The experiment set up three concentration treatments, each with three replicate plots, totaling 150 pepper plants across the three plots. The first foliar spray was applied to the different treatment groups during the seedling stage, and the second foliar spray was applied before flowering. The spray was evenly distributed and did not drip. It was mixed with foliar fertilizer (15-15-15) during spraying. Later agricultural operations were carried out according to farmer practices, and phenotypic trait surveys were conducted regularly. Fifteen plants with consistent growth were selected from each plot for tracking. Basic trait surveys were conducted before spraying. Fifteen days after foliar spraying, stem diameter, plant height, leaf length, and leaf width were measured, and the growth rate was calculated. The number of flowers and fruiting conditions were investigated during the flowering and fruiting periods.

[0126] 3. Experimental results

[0127] The experimental results are shown in Tables 19 and 20. Foliar application of an aqueous Astragalus extract in the field during the seedling stage of peppers promoted growth, while foliar application during the pre-flowering stage promoted flowering and fruiting. Significant promotional effects were observed within the 0.1-1 ppm range, with the optimal concentration being 1 ppm. At the optimal concentration, application during the seedling stage increased pepper seedling stem diameter by 9.77%, plant height by 48.89%, leaf length by 11.89%, and leaf width by 4.70%, significantly outperforming the water control. Application during the pre-flowering stage at the optimal concentration increased the number of pepper flowers by 17.76% and the number of fruiting by 18.18%.

[0128] Table 19 Effect of Astragalus Extract Preparation on Pepper Growth Promotion

[0129] Table 20 Effects of Astragalus Extract Preparations on Flowering and Fruiting of Pepper

[0130] Example 10 Field Test of Astragalus Aqueous Extract Preparation on Growth Regulation of Citrus

[0131] Test crop: Five-year-old citrus (variety: Chunjian)

[0132] 1. Experimental Design

[0133] The experimental sample was the astragalus extract preparation in Example 6 (polysaccharide content of 15000 ppm). After the agent was diluted according to the concentration designed in Table 21, it was sprayed on the leaves of citrus during the fruit swelling period to test the field growth promotion and fruit swelling effect of the astragalus water extract preparation on citrus.

[0134] Table 21 Experimental design

[0135] 2. Experimental methods

[0136] The experiment set up three concentration treatments, and each treatment set up three replicate plots (plot area: 7m*7m=49m 2 ), with each plot spaced one row apart to prevent interference. Four trees were marked in each plot. Foliar spraying was performed on the different treatment groups during the fruit-bearing stage, ensuring even distribution of the spray without dripping. Subsequent agricultural operations and spray application procedures were carried out according to farmer practices. Forty-five days after the experimental treatment, 25 fruits and leaves were randomly selected from four trees in each plot to investigate the citrus fruit's longitudinal and transverse diameters, fruit weight, and leaf SPAD values.

[0137] 3. Experimental results

[0138] The experimental results are shown in Table 22. From the experimental results, it can be seen that the use of Astragalus water extract preparation for foliar spraying during the citrus fruit swelling period can promote the accumulation of chlorophyll in citrus leaves and promote fruit swelling. It shows obvious promoting effects at concentrations of 0.1-1ppm. The optimal concentration is 1ppm. At this concentration, the SPAD value of the leaves can be significantly improved, and the fruit swelling can be promoted. Compared with the clear water control, the single fruit weight increased by 16.98%, the fruit transverse diameter increased by 10.95%, and the fruit longitudinal diameter increased by 14.29%.

[0139] Table 22 Effects of Astragalus membranaceus aqueous extract preparations on citrus growth and fruit swelling

[0140] Example 11 Experiment on Salt Resistance of Cotton Using Astragalus Aqueous Extract

[0141] 1. Experimental Design

[0142] The experimental sample was the astragalus extract preparation in Example 6 (polysaccharide content of 15000 ppm). After the agent was diluted according to the concentration designed in Table 23, cotton was treated to test the salt resistance effect of the astragalus water extract preparation on cotton.

[0143] Table 23 Experimental design

[0144] 2. Experimental methods

[0145] The ratio of nutrient soil and vermiculite is 1:1 (g / g). After mixing evenly, it is used as planting soil. 300g of planting soil is weighed into the planting box, and a 0.8% NaCl aqueous solution is prepared. According to the experimental design, the agent is prepared into different concentrations with the NaCl aqueous solution. 250mL is added to each planting box. The treatment with the addition of 250mL NaCl aqueous solution is the stress control (CK), and the treatment with the addition of 250mL clean water is the non-stress control. Stir evenly and set aside. 20 cotton seeds are evenly sown in each germination box. The planting depth is kept consistent. The lid is covered. Three replicates are set for each treatment. They are placed in a culture room at 25°C for cultivation. The lid is opened when the cotton seedlings emerge from the soil surface. After 5 days, the cotton seedling rate of each treatment group is counted (based on the complete expansion of two cotyledons). After 7 days, the seedlings are taken out for measurement. 15 seedlings are taken from each treatment group to measure plant height and root length.

[0146] 3. Experimental results

[0147] The experimental results are shown in Table 24. It can be seen from the experimental results that the growth of cotton seedlings was significantly inhibited under 0.8% NaCl salt stress, and the seedling rate, plant height and root length indicators were significantly lower than those of the non-stress control group. After treatment with different concentrations of astragalus polysaccharides, the salt stress on cotton seedlings was alleviated, the seedling rate of cotton under salt stress was improved, and the growth of aboveground and underground parts was promoted. The optimal concentration was 1 ppm. Under this concentration treatment, the cotton seedling rate was significantly improved, the plant height growth rate reached 27.55%, and the root length growth rate reached 17.07%.

[0148] Table 24 Effect of Astragalus extract preparation on cotton resistance to salt stress

[0149] Example 12 Experiment on the effect of Astragalus membranaceus aqueous extract on waterlogging resistance of eggplant

[0150] 1. Experimental Design

[0151] The experimental sample was the astragalus extract preparation in Example 6 (polysaccharide content of 15000 ppm). After the agent was diluted according to the concentration designed in Table 25, the eggplant was sprayed on the leaves to test the effect of the astragalus water extract preparation on the waterlogging resistance of eggplant.

[0152] Table 25 Experimental design

[0153] 3. Experimental methods

[0154] Eggplant seedlings (2-3 true leaves) with uniform growth were selected. A total of 5 treatment groups were set up in the experiment. Each treatment group was subjected to waterlogging stress, and the water layer on the soil surface was maintained at 1-2 cm for waterlogging treatment. On the day of waterlogging, each treatment group was sprayed with different concentrations of pesticides to ensure uniform distribution of the liquid. The group sprayed with clean water was used as the stress control (CK) control, and the normal irrigation treatment group was set as the non-stress control (sprayed with clean water). After 14 days, the plant height, stem diameter and aboveground fresh weight of the eggplant seedlings were measured.

[0155] 4. Experimental results

[0156] The experimental results are shown in Table 26. Under normal irrigation conditions, eggplant seedlings achieved a plant height of 25.21 cm, a stem diameter of 3.34 cm, and an aboveground fresh weight of 11.86 g. However, under waterlogging stress, the plant height, stem diameter, and aboveground fresh weight of the eggplant seedlings reached 12.34 cm, 2.13 cm, and 5.73 g, respectively, significantly inhibiting their growth. Foliar application of an Astragalus extract at concentrations ranging from 0.1 to 10 ppm significantly promoted the growth of eggplant seedlings under waterlogging stress, with the optimal concentration being 1 ppm. At this concentration, compared to the control (CK) control, the plant height increased by 30.39%, the stem diameter increased by 28.17%, and the aboveground fresh weight increased by 20.24%.

[0157] Table 26 Effect of Astragalus extract preparation on waterlogging resistance of eggplant

[0158] Example 13 Field Experiment on the Effect of Astragalus Aqueous Extract on High Temperature Resistance of Corn

[0159] Test crop: corn (variety: MY73, 4250 plants / mu)

[0160] 1. Experimental Design

[0161] The experimental sample was the Astragalus extract preparation (polysaccharide content: 15,000 ppm) described in Example 6. After dilution according to the concentrations designed in Table 27, the preparation was foliarly sprayed on corn. High temperatures can affect pollination and reduce yields in corn, and excessively high temperatures can cause bald tips. In this example, foliar spraying was performed on corn during the flowering stage during high temperatures to test the heat resistance of the Astragalus extract preparation in the field.

[0162] Table 27 Experimental design

[0163] 2. Experimental methods

[0164] The experiment set up three concentration treatments, and each treatment set up three replicate plots (plot area: 5m*6m=30m 2), with each plot spaced one row apart to prevent interference. During the flowering stage during high temperatures, foliar spraying was performed on the different treatment groups to ensure even distribution of the solution and prevent dripping. The water-sprayed treatment served as the control (CK) control group. Subsequent agricultural operations and drug application practices followed farmer practices. At corn harvest, 50 plants were randomly selected from each treatment, peeled, and their bald tip rates were calculated. Ten plants with identical leaves were then selected to measure SPAD values ​​and weighed to calculate theoretical yield per mu.

[0165] Theoretical yield per mu (kg) = average weight of single plant (g) × number of plants per mu (4250) / 1000.

[0166] 3. Experimental results

[0167] The experimental results are shown in Table 28. It can be seen from the experimental results that foliar spraying of corn with 0.1-10ppm of Astragalus aqueous extract preparation during high temperature can improve the high temperature resistance of corn to varying degrees. The optimal concentration is 1ppm. At this concentration, the SPAD value of corn leaves can be significantly improved, and its growth can be promoted. The bald tip rate is significantly lower than that of the CK control. At harvest, the per mu yield of corn increases by 6.54% compared with the CK control, indicating that the Astragalus aqueous extract preparation can significantly improve the high temperature resistance of corn.

[0168] Table 28 Effect of Astragalus membranaceus aqueous extract preparation on high temperature resistance of corn

[0169] Example 13 Field Test of Astragalus Extract Preparation on Wheat Resistance to Dry Hot Wind

[0170] Test crop: wheat (variety: Jimai 22)

[0171] 1. Experimental Design

[0172] The experimental sample was the Astragalus extract preparation (polysaccharide content: 15,000 ppm) described in Example 6. After dilution according to the concentrations designed in Table 29, the preparation was sprayed on wheat leaves. Dry, hot winds are a devastating weather condition that affects wheat during its grain-filling and ripening period in northern wheat-growing areas. These winds disrupt the wheat's water balance, hinder metabolic activity, disrupt photosynthesis, shorten the grain-filling process, and severely impact wheat yield. In this example, foliar spraying of wheat during the grain-filling period was performed before the onset of dry, hot winds, based on weather conditions. This was done to test the effectiveness of the Astragalus extract preparation on wheat's resistance to dry, hot winds in the field.

[0173] Table 29 Experimental design

[0174] 2. Experimental methods

[0175] The experiment set up three concentration treatments, and each treatment set up three replicate plots (plot area: 4m*5m=20m 2) A protective row was set up in the middle of each plot to prevent interference. During the grain filling period, depending on weather conditions, foliar spraying was performed on each treatment group two days before the arrival of a dry, hot wind to ensure even distribution of the pesticide solution and prevent dripping. The water-sprayed treatment served as the control (CK) control group. Subsequent agricultural operations and pesticide application practices were carried out according to farmer practices. At harvest, grain number per ear, thousand-grain weight, and yield were measured.

[0176] 3. Experimental results

[0177] The experimental results are shown in Table 30. It can be seen from the experimental results that foliar spraying of 0.1-10 ppm of Astragalus membranaceus aqueous extract on wheat in the filling period 2 days before the arrival of dry hot wind can improve the wheat's resistance to dry hot wind, among which 1 ppm has the best effect. At this concentration, the number of wheat grains per ear increased by 29.30% compared with the CK control, and the thousand-grain weight increased by 22.15%, which had a significant promoting effect on wheat yield, indicating that the Astragalus membranaceus aqueous extract has a significant effect on wheat's resistance to dry hot wind.

[0178] Table 30 Effect of Astragalus membranaceus aqueous extract preparation on wheat resistance to dry hot wind

[0179] Example 14 Field Trial of Astragalus Aqueous Extract Preparation in Preventing Sunburn Damage to Citrus

[0180] Test crop: Citrus (variety: Ehime)

[0181] 1. Experimental Design

[0182] The experimental sample was the Astragalus extract preparation (polysaccharide content: 15,000 ppm) from Example 6. After dilution according to the concentrations designed in Table 31, the preparation was sprayed on the entire citrus plant. Sunburn, a common physiological disease of citrus, is more severe in July and August. Also known as "sun fruit," sunburn is a common citrus disease. Continuous exposure to strong sunlight and intense UV stress stunts the growth of the peel and mesophyll of citrus. The Ehime cultivar has a thin skin and is susceptible to sunburn. In this example, Ehime cultivars were sprayed on the entire plant before high temperatures to test the effectiveness of the Astragalus extract in preventing sunburn in citrus.

[0183] Table 31 Experimental design

[0184] 2. Experimental methods

[0185] The experiment set up three concentration treatments, and each treatment set up three replicate plots (plot area: 7m*7m=49m 2), with one row between each plot to avoid interference with each other, mark 5 fruit trees in each plot, spray the entire citrus tree before high temperature (citrus is not bagged) to ensure that the liquid is evenly distributed without dripping, and use the water spraying treatment as the CK control group. Subsequent agricultural operations and drug use habits are carried out according to farmers' habits.

[0186] 3. Experimental Investigation Method

[0187] During the high-temperature period, citrus sunburn was observed. The number of trees affected by sunburn in each plot (five trees) was counted, and the average sunburn rate was calculated. Sunburned fruit in each plot was graded, and the severity of sunburn damage was compared between treatments. The citrus sunburn grading criteria are shown in Table 32. Sunburned citrus initially appears as small gray-green or yellow-brown spots, which gradually expand to form round or irregular gray-brown dry scars. The affected skin stagnates, becoming hard and rough, and sometimes cracking, resulting in an irregular fruit shape. In mild cases, the burn is limited to the peel. In severe cases, the juice sacs are damaged, resulting in minimal and tasteless juice. The peel hardens, the sacs lose water, and the sac walls become corky, ultimately rendering the fruit worthless.

[0188] Table 32 Grading standards for sunburn damage to citrus

[0189] 4. Experimental results

[0190] The experimental results are shown in Table 33. During the high temperature period, 33.03% of the citrus fruits in the CK control group suffered sunburn. However, treatment with 0.1-10 ppm of Astragalus extract before the high temperature significantly reduced the burn rate. The lowest burn rate was 25.21% at a concentration of 1 ppm, followed by 26.39% at 10 ppm, and 27.96% at 0.1 ppm, significantly lower than the CK control. Regarding the different levels of damage, the CK-treated group suffered mostly Grades III-V, with the highest number of fruits experiencing Grade IV damage, at 11.42%. Treatment with Astragalus polysaccharide extracts also resulted in significantly lower damage levels in Grades II-III than the CK control group. This indicates that applying Astragalus membranaceus water extract before high temperature can prevent sunburn damage to citrus, reduce the degree of burns on citrus during high temperature, and reduce the overall sunburn damage rate of citrus. The best effect is achieved at a concentration of 1 ppm.

[0191] Table 33 Effects of Astragalus membranaceus aqueous extract preparations on the degree of sunburn damage in citrus

[0192] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. Application of astragalus extract containing astragalus polysaccharide in promoting plant growth.

2. An Astragalus extract for promoting plant growth, characterized in that: The astragalus extract comprises at least one of an astragalus water extract, an astragalus water-extracted alcohol precipitate, and an astragalus extract with an ethanol content greater than 0 and less than or equal to 60%. The astragalus extract has astragalus polysaccharide as a marker component, and its concentration is effective within the range of 0.01-1000ppm.

3. The astragalus extract according to claim 2, characterized in that The astragalus extract includes astragalus polysaccharide and at least one saponin, flavonoids, amino acids, alkaloids, monosaccharides, proteins, and trace elements; Preferably, the astragalus water extract comprises astragalus polysaccharides, monosaccharides, proteins, amino acids, Fe, Cu, Zn, K, and Ca as active ingredients.

4. The use according to claim 1, characterized in that: The promoting plant growth includes promoting seed germination, and / or promoting the growth of one or more of roots, stems, leaves, or flowers and fruits.

5. The use according to claim 1, characterized in that: When in use, the astragalus extract is made into an agricultural product, and the agricultural product also includes auxiliary materials, which are one or more of a dispersant, a wetting agent, a binder, an emulsifier, a stabilizer, and a solvent.

6. The use according to claim 5, characterized in that: The dosage form of the product is emulsifiable concentrate, suspension, wettable powder, dust, granule, aqueous solution, mother liquid or mother powder.

7. The application according to claim 6, characterized in that: The agricultural product made from the astragalus extract is used in combination with foliar fertilizer, water-soluble fertilizer and compound fertilizer.

8. The use according to claim 7, characterized in that: When using, a single dose of Astragalus extract or the prepared agricultural product is used to treat seeds, spray leaves or irrigate roots.

9. A plant growth promoter, characterized in that The plant growth promoter comprises astragalus extract, which comprises at least one of astragalus water extract, astragalus water-extracted alcohol precipitation, and astragalus extract with an ethanol content greater than 0 and less than or equal to 60%. Astragalus polysaccharide is used as a marker component in the astragalus extract, and is effective within a concentration range of 0.01-1000ppm.

10. Application of astragalus extract containing astragalus polysaccharide in regulating plant stress resistance, the astragalus extract comprising at least one of astragalus water extract, astragalus water-extracted alcohol precipitate, and astragalus extract with an ethanol content greater than 0 and less than or equal to 60%, the astragalus extract having astragalus polysaccharide as a marker component and being effective at a concentration in the range of 0.01-1000ppm.

11. The use according to claim 10, characterized in that: The stress resistance is at least one of cold resistance, drought resistance, salt resistance, high temperature resistance, waterlogging resistance, and UV damage resistance.

12. The use or growth promoter according to any one of claims 1 to 11, characterized in that: The plants include tobacco, corn, lettuce, wheat, pepper, Chinese cabbage, lettuce, bok choy, tomato, citrus, kiwi, cherry, pear, apple, cotton, cucumber and eggplant.

Images