Procyanidine compound hard capsule for beauty-maintaining and young-keeping health-care food as well as preparation process and application of procyanidine compound hard capsule

Abstract

The invention discloses a procyanidine compound hard capsule for a health food capable of maintaining beauty and keeping young. The procyanidine compound hard capsule is prepared from the following components: a procyanidine extract, chitosan, vitamins, collagen, hyaluronic acid, a lotus leaf extract, astragalus polysaccharide, casein phosphopeptides, D-mannitol, starch, magnesium stearate and gelatin. The invention relates to a production process and application of a procyanidine compound hard capsule for beauty and young keeping health food, and the production process comprises the following steps: step 1, adjusting a formula proportion by using a component proportion optimization model to obtain an optimal preparation effect; 2, solution preparation is conducted on all the components, and the prepared solutions are mixed together; step 3, drying the procyanidine compound solution by adopting low-temperature vacuum drying; and 4, metering the dried procyanidine compound powder by using a capsule filling machine, and sub-packaging the procyanidine compound powder into medicinal hard capsules. The beauty-maintaining, young-keeping and health-care effects are greatly improved.

Description

Technical Field

[0001] The present invention is based on the fields of biotechnology and pharmaceuticals, and more specifically relates to a proanthocyanidin complex hard capsule for beauty and health food, and a production process and application thereof. Background Art

[0002] As the pace of modern life accelerates, people are paying more and more attention to health and beauty. The market demand for health foods containing proanthocyanidins is increasing, especially those that are easy to carry, easy to take and have significant effects.

[0003] However, the existing proanthocyanidin complex hard capsules used for beauty and health care foods have the following shortcomings:

[0004] (1) Proanthocyanidins are abundant in plants, but the extracted and purified proanthocyanidins are low in content and purity;

[0005] (2) Proanthocyanidins are sensitive to environmental conditions and are prone to adverse reactions such as oxidative degradation, which results in poor stability of some proanthocyanidin products during storage and transportation, affecting the shelf life and effect of the products;

[0006] (3) Existing proanthocyanidin complex hard capsules have problems of uneven filling and slow disintegration;

[0007] (4) Proanthocyanidin products have the problem of low bioavailability.

[0008] Based on the above problems, the present invention discloses a proanthocyanidin complex hard capsule for beauty and health food, and its production process and application. By optimizing the raw material ratio, the problems existing in the prior art are solved, the bioavailability, stability, antioxidant and beauty and health effects of the proanthocyanidin complex hard capsule are improved, while the production cost and complexity are reduced to meet market demand. Summary of the invention

[0009] In view of the deficiencies of the above-mentioned technologies and the deficiencies of traditional proanthocyanidin complex hard capsules in production, bioavailability, stability, antioxidant properties and beauty and health care effects, the present invention discloses a proanthocyanidin complex hard capsule for beauty and health care food and its production process and application, which improves the bioavailability, stability, antioxidant properties and beauty and health care effects of the proanthocyanidin complex hard capsule, while reducing production costs and complexity.

[0010] A proanthocyanidin complex hard capsule for beauty and health food, comprising the following ingredients:

[0011] 60-80 parts of proanthocyanidin extract, 5-10 parts of chitosan, 1-5 parts of vitamin C, 1-5 parts of vitamin E, 2-10 parts of collagen, 0.5-5 parts of hyaluronic acid, 1-10 parts of lotus leaf extract, 1-5 parts of astragalus polysaccharide, 0.1-1 parts of casein phosphopeptide, 0.2-2 parts of D-mannitol, 0.5-2 parts of starch, 0.2-2 parts of magnesium stearate and 30-50 parts of gelatin;

[0012] The proanthocyanidin complex hard capsule adjusts the proportion of each component through a component ratio optimization model, thereby improving the stability, embedding rate, bioavailability and antioxidant activity of the proanthocyanidin complex hard capsule.

[0013] As a further embodiment of the present invention, the extraction process of the proanthocyanidin extract is:

[0014] (1) preparing raw materials, selecting grape seeds as raw materials, crushing or grinding the grape seeds to increase the contact area of ​​the solvent and improve the extraction efficiency;

[0015] (2) mixing the crushed grape seeds with a 60% ethanol solution in a ratio of 1:40, performing hot reflux extraction at a temperature of 65 to 70° C. for 1.5 to 2.5 hours, and repeating the extraction 4 to 5 times;

[0016] (3) filtering the extract to remove insoluble matter, and concentrating the filtered filtrate by a rotary evaporator. During the concentration process, the temperature is set to 45-65° C. and the pressure is set to 60-100 Pa to remove part of the solvent to obtain anthocyanidin concentrate;

[0017] (4) purifying the proanthocyanidin concentrate by using AB-8 macroporous adsorption resin to obtain a purified proanthocyanidin extract; and drying the extract at a temperature of 40 to 50° C.

[0018] As a further embodiment of the present invention, during the purification process of the proanthocyanidin concentrate by the AB-8 macroporous adsorption resin, the pH value of the proanthocyanidin concentrate is adjusted to between 4.5 and 7.0, the AB-8 macroporous adsorption resin is loaded into a chromatographic column, and the proanthocyanidin concentrate is passed through a resin bed at a flow rate of 3 to 6 mL / min for adsorption treatment; an ethanol solution with a concentration of 60% is selected to elute the proanthocyanidins adsorbed on the resin, and the 60% ethanol solution is passed through the resin bed in batches at different flow rates to elute the proanthocyanidins adsorbed on the resin, and the process is repeated 3 to 5 times until the proanthocyanidins are completely eluted; the collected proanthocyanidin extract is subjected to purity analysis by high performance liquid chromatography HPLC to observe whether the purity reaches 90% or more.

[0019] As a further embodiment of the present invention, the component ratio optimization model construction process is:

[0020] (1) Target setting stage: determining the optimized performance indicators, which include at least stability, encapsulation efficiency, bioavailability and antioxidant activity; setting proportion constraints according to the physical, chemical and biological limitations of the component ratio;

[0021] (2) In the particle swarm initialization stage, a certain number of particles are randomly generated in the solution space, each particle represents a different component ratio scheme, and each particle has a particle position and a particle speed. The particle position is the component ratio, and the particle speed is the search direction. The mathematical expression of the initialization particle position component ratio is:

[0022]

[0023] In formula (1), x represents the composition ratio, i represents the i-th particle, and j represents the number of combinations between the components. Represents the initial position of the i-th particle;

[0024] The mathematical expression for initializing particle velocity is:

[0025]

[0026] In formula (2), It represents the initialization particle search speed of the ith particle, and v represents the search speed;

[0027] (3) Fitness evaluation phase: updating the fitness value of each particle to evaluate its quality;

[0028] (4) In the particle swarm update phase, the speed update formula usually includes an inertia term, a cognitive term, and a social term; the inertia term maintains the particle's previous motion trend, the cognitive term is the particle's tendency to move toward its own historical optimal position, and the social term is the particle's tendency to move toward the group's historical optimal position;

[0029] The mathematical operation expression for updating particle velocity is:

[0030]

[0031] In formula (3), T is the number of iterations, M is the inertia weight, and r 1 and r 2 is a random number, a 1 and a 2 is the acceleration factor, is the optimal formulation ratio of particles at the Tth iteration, is the global optimal formulation ratio of the particle at the Tth iteration;

[0032] The mathematical operation expression for updating the particle position composition ratio is:

[0033]

[0034] (5) In the global optimal solution search phase, the fitness values ​​of all particles are compared and the global optimal solution is updated, that is, the best ingredient ratio scheme found so far; the mathematical operation expression for updating the global optimal formula ratio of the particle swarm is:

[0035]

[0036] (6) During the verification and evaluation phase, the optimized ingredient ratio scheme is verified under laboratory conditions to test the stability, encapsulation efficiency, bioavailability and antioxidant activity performance indicators; based on the verification results and actual needs, the optimization model is feedback adjusted to further optimize the ingredient ratio scheme.

[0037] As a further embodiment of the present invention, a production process and application of a proanthocyanidin complex hard capsule for beauty and health food, the preparation process of the proanthocyanidin complex hard capsule is as follows:

[0038] Step 1, using the ingredient ratio optimization model to adjust the formula ratio to obtain the optimal formulation effect, and weighing proanthocyanidin extract, chitosan, vitamin C, vitamin E, collagen, hyaluronic acid, lotus leaf extract, astragalus polysaccharide, casein phosphopeptide and D-mannitol according to the formula ratio;

[0039] Step 2: Prepare a solution of each component, mix the prepared solution together, and stir it for 2 to 4 minutes at a temperature of ℃ to obtain a proanthocyanidin complex solution;

[0040] Step 3, drying the proanthocyanidin complex solution by low-temperature vacuum drying, in the pre-freezing stage, quickly freezing the proanthocyanidin complex solution at a low temperature of -40 to -50°C for 3 to 5 hours, until the water in the solution is completely solidified into ice crystals; in the vacuum drying stage, the pressure is 10 to 13 Pa, the temperature is below 0°C, and the drying time is 4 to 6 hours to obtain a proanthocyanidin complex powder;

[0041] Step 4: The dried proanthocyanidin complex powder is measured by a capsule filling machine and divided into medicinal hard capsules to obtain the proanthocyanidin complex hard capsules, each hard capsule containing 0.5 g of the substance.

[0042] As a further embodiment of the present invention, the solution preparation process is: dissolving the proanthocyanidin extract and the lotus leaf extract in edible alcohol with a concentration of 2% to prepare a proanthocyanidin aqueous solution and a lotus leaf extract aqueous solution; dissolving chitosan and vitamin E in an aqueous acid solution with a concentration of less than 5% to prepare a chitosan aqueous solution and a vitamin E aqueous solution; mixing vitamin C, astragalus polysaccharide and D-mannitol with water and stirring until completely dissolved to obtain a vitamin C solution, an astragalus polysaccharide solution and a D-mannitol solution.

[0043] As a further embodiment of the present invention, the components of the hard capsule include 30-50 parts of gelatin, 0.1-1.5 parts of glycerol, 1.5-4 parts of starch, 0.2-2 parts of magnesium stearate and 5-15 parts of water; the gelatin is biocompatible and degradable, and is safely dissolved and absorbed in the human body; the glycerol acts as a plasticizer to increase the softness and plasticity of the gelatin and maintain the humidity of the hard capsule; the magnesium stearate acts as a lubricant to reduce friction and adhesion of the hard capsule during the preparation process; the starch adjusts the hardness and brittleness of the hard capsule. When the starch content is 12-15%, the hardness range of the hard capsule is 18-25N, when the starch content is 8-11%, the hardness range of the hard capsule is 11-17N, and when the starch content is 3-7%, the hardness range of the hard capsule is 5-10N.

[0044] As a further embodiment of the present invention, the proanthocyanidin complex hard capsule utilizes the antioxidant property of proanthocyanidins to remove excess free radicals in the body and reduce cell damage; the chitosan is used as a stabilizer and carrier to form a mixture with proanthocyanidins to enhance moisture retention, film-forming properties and biocompatibility; the D-mannitol is used as a filler and sweetener to improve the taste and texture of the capsule; the hyaluronic acid absorbs and retains a large amount of water, keeps the skin hydrated, and increases the beauty effect.

[0045] Positive beneficial effects

[0046] A proanthocyanidin complex hard capsule for beauty and health food, and its production process and application. The proanthocyanidin complex promotes skin metabolism through its antioxidant effect, improves skin dullness and dryness discomfort symptoms, makes the skin more delicate and smooth, and promotes the formation of collagen, which helps to maintain the elasticity and firmness of the skin, and can also promote cardiovascular health and protect eyesight. During the production process, the proportion of each component is adjusted through an ingredient optimization ratio model to improve the solubility, permeability and absorbability of proanthocyanidins, improve the bioavailability in the body, and select auxiliary ingredients with synergistic antioxidant effects. The components are compounded with proanthocyanidins in a certain ratio to form a complex with stronger antioxidant ability, improve antioxidant activity, and improve stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work, among which:

[0048] Figure 1 The present invention is a specific flow chart of the production process of a proanthocyanidin complex hard capsule for beauty and health food;

[0049] Figure 2 This is a schematic diagram of the raw material ratio of a proanthocyanidin complex hard capsule for beauty and health food of the present invention;

[0050] Figure 3 This is a schematic diagram of the proportion of raw materials for the hard capsule wall material of a proanthocyanidin complex hard capsule for beauty and health food of the present invention. DETAILED DESCRIPTION

[0051] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0052] Example 1

[0053] A proanthocyanidin complex hard capsule for beauty and health food, comprising the following components: proanthocyanidin extract, chitosan, vitamin C, vitamin E, collagen, hyaluronic acid, lotus leaf extract, astragalus polysaccharide, casein phosphopeptide, D-mannitol, starch, magnesium stearate and gelatin;

[0054] The proanthocyanidin complex hard capsule adjusts the proportion of each component through a component ratio optimization model, thereby improving the stability, embedding rate, bioavailability and antioxidant activity of the proanthocyanidin complex hard capsule.

[0055] In the above embodiment, the extraction process of the proanthocyanidin extract is:

[0056] (1) preparing raw materials, selecting grape seeds as raw materials, crushing or grinding the grape seeds to increase the contact area of ​​the solvent and improve the extraction efficiency;

[0057] (2) mixing the crushed grape seeds with a 60% ethanol solution in a ratio of 1:40, performing hot reflux extraction at a temperature of 65° C. for 2 hours, and repeating the extraction 4 to 5 times;

[0058] (3) filtering the extract to remove insoluble matter, and concentrating the filtered filtrate by a rotary evaporator. During the concentration process, the temperature is set to 55° C. and the pressure is set to 80 Pa to remove part of the solvent to obtain anthocyanidin concentrate;

[0059] (4) purifying the proanthocyanidin concentrate using AB-8 macroporous adsorption resin to obtain a purified proanthocyanidin extract; and drying the extract at a temperature of 45° C.

[0060] In the above embodiment, during the purification of the proanthocyanidin concentrate by the AB-8 macroporous adsorption resin, the pH value of the proanthocyanidin concentrate is adjusted to 6.0, the AB-8 macroporous adsorption resin is loaded into the chromatographic column, and the proanthocyanidin concentrate is adsorbed through the resin bed; an ethanol solution with a concentration of 60% is selected to elute the proanthocyanidin adsorbed on the resin, and the 60% ethanol solution is passed through the resin bed at a flow rate of 3mL / min, 4mL / min, 5mL / min and 6mL / min in batches to elute the proanthocyanidin adsorbed on the resin, and the process is repeated 3 to 5 times until the proanthocyanidin is completely eluted; the collected proanthocyanidin extract is analyzed for purity by high performance liquid chromatography HPLC to observe whether the purity reaches more than 90%. High performance liquid chromatography HPLC uses a C18 column with a specification of 4.6mm×250mm and a filler particle size of 5μm; the proanthocyanidin concentrate is subjected to adsorption tests in batches at different flow rates, and the test results are shown in Table 1.

[0061] Table 1 Effect of different flow rates on purification results

[0062] Flow rate / mL / min Extraction rate / % purity / % Eligibility 3 43.9 89 no 4 63.7 95 yes 5 56.3 91 yes 6 55.1 87 no

[0063] It can be seen from Table 1 that when the flow rate of the 60% ethanol solution is 4 mL / min, the extraction rate of the proanthocyanidin extract is the highest and the extraction purity is the highest, that is, the subsequent experiments are carried out at a flow rate of 4 mL / min.

[0064] Example 2

[0065] In the above embodiment, the extraction process of the proanthocyanidin extract is:

[0066] (1) preparing raw materials, selecting grape seeds as raw materials, crushing or grinding the grape seeds to increase the contact area of ​​the solvent and improve the extraction efficiency;

[0067] (2) mixing the crushed grape seeds with a 60% ethanol solution in a ratio of 1:40, performing hot reflux extraction at a temperature of 65° C. for 2 hours, and repeating the extraction 4 to 5 times;

[0068] (3) filtering the extract to remove insoluble matter, and concentrating the filtered filtrate by a rotary evaporator. During the concentration process, the temperature was set to 45° C., 50° C., 55° C., 60° C., and 65° C., and the pressure was set to 80 Pa to remove part of the solvent and obtain a proanthocyanidin concentrate;

[0069] (4) purifying the proanthocyanidin concentrate using AB-8 macroporous adsorption resin to obtain a purified proanthocyanidin extract; and drying the extract at a temperature of 45° C.

[0070] In the above embodiment, during the purification of the proanthocyanidin concentrate by the AB-8 macroporous adsorption resin, the pH value of the proanthocyanidin concentrate is adjusted to between 4.5 and 7.0, the AB-8 macroporous adsorption resin is loaded into the chromatographic column, and an ethanol solution with a concentration of 60% is selected to elute the proanthocyanidins adsorbed on the resin, and the 60% ethanol solution is passed through the resin bed in batches at a flow rate of 4mL / min to elute the proanthocyanidins adsorbed on the resin, and the process is repeated 3 to 5 times until the proanthocyanidins are completely eluted; the collected proanthocyanidin extract is subjected to purity analysis by high performance liquid chromatography HPLC to observe whether the purity reaches more than 90%. High performance liquid chromatography HPLC uses a C18 column with a specification of 4.6mm×250mm and a filler particle size of 5μm. The results of the effect of temperature on the purification test are shown in Table 2.

[0071] Table 2 Effect of temperature on purification test results

[0072] Temperature / ℃ Extraction rate / % purity / % Eligibility 45 43.1 85 no 50 50.6 88 no 55 56.3 91 yes 60 65.2 95 yes 65 55.1 92 yes

[0073] It can be seen from Table 2 that when the temperature is 60°C, the extraction rate of proanthocyanidins extract is the highest and the extraction purity is the highest.

[0074] Example 3

[0075] In the above embodiment, the component ratio optimization model construction process is:

[0076] (1) Target setting stage: determining the optimized performance indicators, which include at least stability, encapsulation efficiency, bioavailability and antioxidant activity; setting proportion constraints according to the physical, chemical and biological limitations of the component ratio;

[0077] (2) In the particle swarm initialization stage, a certain number of particles are randomly generated in the solution space, each particle represents a different component ratio scheme, and each particle has a particle position and a particle speed. The particle position is the component ratio, and the particle speed is the search direction. The mathematical expression of the initialization particle position component ratio is:

[0078]

[0079] In formula (1), x represents the composition ratio, i represents the i-th particle, and j represents the number of combinations between the components. Represents the initial position of the i-th particle;

[0080] The mathematical expression for initializing particle velocity is:

[0081]

[0082] In formula (2), It represents the initialization particle search speed of the ith particle, and v represents the search speed;

[0083] (3) Fitness evaluation phase: updating the fitness value of each particle to evaluate its quality;

[0084] (4) In the particle swarm update phase, the speed update formula usually includes an inertia term, a cognitive term, and a social term; the inertia term maintains the particle's previous motion trend, the cognitive term is the particle's tendency to move toward its own historical optimal position, and the social term is the particle's tendency to move toward the group's historical optimal position;

[0085] The mathematical operation expression for updating particle velocity is:

[0086]

[0087] In formula (3), t is the number of iterations, M is the inertia weight, and r 1 and r 2 is a random number, a 1 and a 2 is the acceleration factor, is the optimal formulation ratio of particles at the Tth iteration, is the global optimal formulation ratio of the particle at the Tth iteration;

[0088] The mathematical operation expression for updating the particle position composition ratio is:

[0089]

[0090] (5) In the global optimal solution search phase, the fitness values ​​of all particles are compared and the global optimal solution is updated, that is, the best component ratio scheme found so far; the mathematical operation expression for updating the global optimal solution of the particle swarm is:

[0091]

[0092] (6) During the verification and evaluation phase, the optimized ingredient ratio scheme is verified under laboratory conditions to test the stability, encapsulation efficiency, bioavailability and antioxidant activity performance indicators; based on the verification results and actual needs, the optimization model is feedback adjusted to further optimize the ingredient ratio scheme.

[0093] In a specific embodiment, the original mathematical operation expression for updating the particle velocity is:

[0094]

[0095] The original mathematical operation expression for updating the particle position composition ratio is:

[0096]

[0097] The original mathematical operation expression for updating the global optimal solution of the particle swarm is:

[0098]

[0099] The inertia weight M controls the influence of the previous speed on the current speed. represents the velocity of the particle at the Tth iteration, Maintaining the previous movement trend of the particle, represents the tendency of particles to move toward their historical optimal position, Indicates the tendency of particles to move toward the historical optimal position of the group, a 1 The acceleration factor is used to adjust the step size to approach the historical optimal position. 2 Used to adjust the step size of the particle approaching the global optimal position, r 1 and r 2 Used to increase the randomness of the search.

[0100] In the above embodiment, a production process and application of a proanthocyanidin complex hard capsule for beauty and health food: the preparation process of the proanthocyanidin complex hard capsule is:

[0101] Step 1, using the ingredient ratio optimization model to adjust the formula ratio to obtain the optimal formulation effect, and weighing proanthocyanidin extract, chitosan, vitamin C, vitamin E, collagen, hyaluronic acid, lotus leaf extract, astragalus polysaccharide, casein phosphopeptide and D-mannitol according to the formula ratio;

[0102] Step 2: Prepare a solution of each component, mix the prepared solution together, and stir it for 3 minutes at a temperature of ℃ to obtain a proanthocyanidin complex solution;

[0103] Step 3, the proanthocyanidin complex solution is dried by low-temperature vacuum drying. In the pre-freezing stage, the proanthocyanidin complex solution is quickly frozen at a low temperature of -45°C for 5 hours until the water in the solution is completely solidified into ice crystals; in the vacuum drying stage, the pressure is 10Pa, the temperature is below 0°C, and the drying time is 5 hours to obtain the proanthocyanidin complex powder;

[0104] Step 4: The dried proanthocyanidin complex powder is measured by a capsule filling machine and divided into medicinal hard capsules to obtain the proanthocyanidin complex hard capsules, each hard capsule containing 0.5 g of the substance.

[0105] The bioavailability, antioxidant activity and stability of the proanthocyanidin complex hard capsules were improved by the ingredient ratio optimization model. The test results are shown in Table 3.

[0106] Table 3 Experimental results of proanthocyanidin complex hard capsules

[0107] project Before optimization After optimization Eligibility Bioavailability / % 62 90 yes Free radical scavenging rate / % 74 91 yes Remaining activity after three months of storage / % 79 97 yes

[0108] As shown in Table 3, the bioavailability, antioxidant activity and stability of the proanthocyanidin complex hard capsules were greatly improved by adjusting the proportion of each component through the component ratio optimization model.

[0109] The toxicity test of proanthocyanidin capsules was carried out on mice, with 20 male and female mice each, and the observation was carried out for 30 days. The test results are shown in Table 4.

[0110] Table 4 Toxicity test results

[0111] gender mortality rate / % How to eat Any adverse reactions? male 0 oral none female 0 oral none

[0112] It can be seen from Table 4 that the mice grew and developed well without any adverse reactions, indicating that the proanthocyanidin hard capsules had no obvious toxic side effects.

[0113] In a specific embodiment, the implementation process of the component ratio optimization model is:

[0114] (1) Initialize the population, including the upper and lower limits of the search space, two learning factors, the maximum number of iterations of the algorithm, and the upper and lower limits of the velocity of each particle; randomly initialize the position and velocity of each particle in the population;

[0115] (2) Calculate the fitness value of each particle according to the fitness function, save the optimal position of each particle, save the individual best fitness value and the best position of the group so far;

[0116] (3) Update the speed and position according to the speed and position update formula;

[0117] (4) Calculate the fitness value of each particle after the update, and compare the best fitness value of each particle with the fitness value at its historical optimal position. If it is better, use its current position as the optimal position of the particle;

[0118] (5) For each particle, compare the fitness value corresponding to its best position with the best fitness value of the population. If it is better, update the optimal position and the best fitness value of the population.

[0119] (6) Determine whether the searched structure meets the stopping condition. If so, output the optimal value. Otherwise, jump to (3) and continue running until the condition is met.

[0120] Example 4

[0121] In the above embodiment, the preparation process of the proanthocyanidin complex hard capsule is as follows:

[0122] Step 1, using the ingredient ratio optimization model to adjust the formula ratio to obtain the optimal formulation effect, and weighing proanthocyanidin extract, chitosan, vitamin C, vitamin E, collagen, hyaluronic acid, lotus leaf extract, astragalus polysaccharide, casein phosphopeptide and D-mannitol according to the formula ratio;

[0123] Step 2: Prepare a solution of each component, mix the prepared solution together, and stir it for 3 minutes at a temperature of ℃ to obtain a proanthocyanidin complex solution;

[0124] Step 3, the proanthocyanidin complex solution is dried by low-temperature vacuum drying. In the pre-freezing stage, the proanthocyanidin complex solution is quickly frozen at a low temperature of -45°C for 5 hours until the water in the solution is completely solidified into ice crystals; in the vacuum drying stage, the pressure is 10Pa, the temperature is below 0°C, and the drying time is 5 hours to obtain the proanthocyanidin complex powder;

[0125] Step 4: The dried proanthocyanidin complex powder is measured by a capsule filling machine and divided into medicinal hard capsules to obtain the proanthocyanidin complex hard capsules, each hard capsule containing 0.5 g of the substance.

[0126] In the above embodiment, the solution preparation process is as follows: dissolving the proanthocyanidin extract and the lotus leaf extract in edible alcohol with a concentration of 2% to prepare a proanthocyanidin aqueous solution and a lotus leaf extract aqueous solution; dissolving chitosan and vitamin E in an aqueous solution of an acid with a concentration of less than 5% to prepare a chitosan aqueous solution and a vitamin E aqueous solution; mixing vitamin C, astragalus polysaccharide and D-mannitol with water and stirring until completely dissolved to obtain a vitamin C solution, an astragalus polysaccharide solution and a D-mannitol solution.

[0127] The voluntary test subjects were randomly divided into Group A and Group B. Group A was the control group, and the test subjects took placebos. Group B was the test group, and the test subjects took proanthocyanidin hard capsules. The skin moisture was tested for one month. The test results are shown in Table 5.

[0128] Table 5 Skin moisture test results

[0129]

[0130] It can be seen from Table 5 that, compared with Group A and Group B, the skin moisture of the test group who took the proanthocyanidin complex hard capsules increased significantly, indicating that the proanthocyanidin hard capsules of the present invention have good beauty and skin care effects.

[0131] Example 5

[0132] In the above embodiment, the preparation process of the proanthocyanidin complex hard capsule is as follows:

[0133] Step 1, using the ingredient ratio optimization model to adjust the formula ratio to obtain the optimal formulation effect, and weighing proanthocyanidin extract, chitosan, vitamin C, vitamin E, collagen, hyaluronic acid, lotus leaf extract, astragalus polysaccharide, casein phosphopeptide and D-mannitol according to the formula ratio;

[0134] Step 2: Prepare a solution of each component, mix the prepared solution together, and stir it for 3 minutes at a temperature of ℃ to obtain a proanthocyanidin complex solution;

[0135] Step 3, the proanthocyanidin complex solution is dried by low-temperature vacuum drying. In the pre-freezing stage, the proanthocyanidin complex solution is quickly frozen at a low temperature of -45°C for 5 hours until the water in the solution is completely solidified into ice crystals; in the vacuum drying stage, the pressure is 10Pa, the temperature is below 0°C, and the drying time is 5 hours to obtain the proanthocyanidin complex powder;

[0136] Step 4: The dried proanthocyanidin complex powder is measured by a capsule filling machine and divided into medicinal hard capsules to obtain the proanthocyanidin complex hard capsules, each hard capsule containing 0.5 g of the substance.

[0137] In the above embodiment, the solution preparation process is as follows: dissolving the proanthocyanidin extract and the lotus leaf extract in edible alcohol with a concentration of 2% to prepare a proanthocyanidin aqueous solution and a lotus leaf extract aqueous solution; dissolving chitosan and vitamin E in an aqueous solution of an acid with a concentration of less than 5% to prepare a chitosan aqueous solution and a vitamin E aqueous solution; mixing vitamin C, astragalus polysaccharide and D-mannitol with water and stirring until completely dissolved to obtain a vitamin C solution, an astragalus polysaccharide solution and a D-mannitol solution.

[0138] The voluntary subjects were randomly divided into Group A and Group B. Group A was the control group, and the subjects took placebo. Group B was the test group, and the subjects took proanthocyanidin hard capsules. The macular area and macular depth were tested for one month. The test results are shown in Table 6.

[0139] Table 6 Results of macular area and macular depth detection

[0140] Serial number <![CDATA[Change in macular area / mm 2 > Color spot depth change / bit Group A +0.10 +0.09 Group B -29.93 -0.58

[0141] It can be seen from Table 6 that the macular area and the depth of the spots of the subjects who took proanthocyanidin capsules were significantly reduced, indicating that proanthocyanidin greatly inhibits the activity of tyrosinase, thereby reducing the synthesis of melanin and having a good beauty and skin care effect.

[0142] Example 6

[0143] In the above embodiment, the proanthocyanidin complex hard capsule utilizes the antioxidant property of proanthocyanidins to remove excess free radicals in the body and reduce cell damage; the chitosan is used as a stabilizer and carrier to form a mixture with proanthocyanidins to enhance moisture retention, film-forming properties and biocompatibility; the D-mannitol is used as a filler and sweetener to improve the taste and texture of the capsule; the hyaluronic acid absorbs and retains a large amount of water, keeps the skin hydrated, and increases the beauty effect.

[0144] In the above embodiment, the components of the hard capsule include 30-50 parts of gelatin, 0.1-1.5 parts of glycerol, 1.5-4 parts of starch, 0.2-2 parts of magnesium stearate and 5-15 parts of water; the gelatin has biocompatibility and degradability, and is safely dissolved and absorbed in the human body; the glycerol acts as a plasticizer to increase the softness and plasticity of the gelatin and maintain the humidity of the hard capsule; the magnesium stearate acts as a lubricant to reduce the friction and adhesion of the hard capsule during the preparation process; the starch adjusts the hardness and brittleness of the hard capsule. When the starch content is 12-15%, the hardness range of the hard capsule is 18-25N, when the starch content is 8-11%, the hardness range of the hard capsule is 11-17N, and when the starch content is 3-7%, the hardness range of the hard capsule is 5-10N. The appropriate core-wall ratio is selected through the component ratio optimization model, and the test results are shown in Table 7.

[0145] Table 7 Effect of core-to-wall ratio on embedding efficiency

[0146] Core-to-wall ratio Embedding rate / % 1:5 89 2:5 95 3:5 90 4:5 79

[0147] It can be seen from Table 7 that when the core-to-wall ratio is 2:5, the embedding rate of the hard capsule reaches 95%. The ingredient ratio optimization model greatly improves the embedding rate, greatly reduces the test time, and improves production efficiency.

[0148] Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that these specific embodiments are only illustrative, and those skilled in the art may omit, replace, and change the details of the above methods and systems in various ways without departing from the principles and essence of the present invention. For example, merging the above method steps so as to perform substantially the same functions in substantially the same manner to achieve substantially the same results is within the scope of the present invention. Therefore, the scope of the present invention is limited only by the appended claims.

Claims

1. A proanthocyanidin complex hard capsule for beauty and health food, characterized in that: The following ingredients are used: 60-80 parts of proanthocyanidin extract, 5-10 parts of chitosan, 1-5 parts of vitamin C, 1-5 parts of vitamin E, 2-10 parts of collagen, 0.5-5 parts of hyaluronic acid, 1-10 parts of lotus leaf extract, 1-5 parts of astragalus polysaccharide, 0.1-1 parts of casein phosphopeptide, 0.2-2 parts of D-mannitol, 0.5-2 parts of starch, 0.2-2 parts of magnesium stearate and 30-50 parts of gelatin; The proportion of each component of the proanthocyanidin complex hard capsule is adjusted through the component ratio optimization model.

2. The proanthocyanidin complex hard capsule for beauty and health food according to claim 1, characterized in that: The extraction process of the proanthocyanidins extract is: (1) preparing raw materials, selecting grape seeds as raw materials, crushing or grinding the grape seeds to increase the contact area of ​​the solvent and improve the extraction efficiency; (2) mixing the crushed grape seeds with a 60% ethanol solution in a ratio of 1:40, performing hot reflux extraction at a temperature of 65 to 70° C. for 1.5 to 2.5 hours, and repeating the extraction 4 to 5 times; (3) filtering the extract to remove insoluble matter, and concentrating the filtered filtrate by a rotary evaporator. During the concentration process, the temperature is set to 45-65° C. and the pressure is set to 60-100 Pa to remove part of the solvent to obtain anthocyanidin concentrate; (4) purifying the proanthocyanidin concentrate by using AB-8 macroporous adsorption resin to obtain a purified proanthocyanidin extract; and drying the extract at a temperature of 40 to 50° C.

3. The proanthocyanidin complex hard capsule for beauty and skin care health food according to claim 2, characterized in that: During the purification process of the proanthocyanidin concentrate by the AB-8 macroporous adsorption resin, the pH value of the proanthocyanidin concentrate is adjusted to between 4.5 and 7.0, the AB-8 macroporous adsorption resin is loaded into a chromatographic column, and the proanthocyanidin concentrate is passed through a resin bed at a flow rate of 3 to 6 mL / min for adsorption treatment; an ethanol solution with a concentration of 60% is selected to elute the proanthocyanidin adsorbed on the resin, and the 60% ethanol solution is passed through the resin bed in batches at different flow rates to elute the proanthocyanidin adsorbed on the resin, and the process is repeated 3 to 5 times until the proanthocyanidin is completely eluted; the collected proanthocyanidin extract is subjected to purity analysis by high performance liquid chromatography HPLC to observe whether the purity reaches more than 90%.

4. The proanthocyanidin complex hard capsule for beauty and health food according to claim 1, characterized in that: The component ratio optimization model construction process is as follows: (1) Target setting stage: determining the optimized performance indicators, which include at least stability, encapsulation efficiency, bioavailability and antioxidant activity; setting proportion constraints according to the physical, chemical and biological limitations of the component ratio; (2) In the particle swarm initialization stage, a certain number of particles are randomly generated in the solution space, each particle represents a different component ratio scheme, and each particle has a particle position and a particle speed. The particle position is the component ratio, and the particle speed is the search direction. The mathematical expression of the initialization particle position component ratio is: In formula (1), x represents the composition ratio, i represents the i-th particle, and j represents the number of combinations between the components. Represents the initial position of the ith particle; The mathematical expression for initializing particle velocity is: In formula (2), It represents the initialization particle search speed of the ith particle, and v represents the search speed; (3) Fitness evaluation phase: updating the fitness value of each particle to evaluate its quality; (4) In the particle swarm update phase, the speed update formula usually includes an inertia term, a cognitive term, and a social term; the inertia term maintains the particle's previous motion trend, the cognitive term is the particle's tendency to move toward its own historical optimal position, and the social term is the particle's tendency to move toward the group's historical optimal position; The mathematical operation expression for updating particle velocity is: In formula (3), T is the number of iterations, M is the inertia weight, r1 and r2 are random numbers, a1 and a2 are acceleration factors, is the optimal formulation ratio of particles at the Tth iteration, is the global optimal formulation ratio of the particle at the Tth iteration; The mathematical operation expression for updating the particle position composition ratio is: (5) In the global optimal solution search phase, the fitness values ​​of all particles are compared and the global optimal solution is updated, that is, the best ingredient ratio scheme found so far; the mathematical operation expression for updating the global optimal formula ratio of the particle swarm is: (6) During the verification and evaluation phase, the optimized ingredient ratio scheme is verified under laboratory conditions to test the stability, encapsulation efficiency, bioavailability and antioxidant activity performance indicators; based on the verification results and actual needs, the optimization model is feedback adjusted to further optimize the ingredient ratio scheme.

5. A process for preparing a proanthocyanidin complex hard capsule for beauty and health food, characterized in that: The proanthocyanidin complex hard capsule for beauty and health food according to claim 1-4 is characterized in that the preparation process of the proanthocyanidin complex hard capsule is as follows: Step 1, using the ingredient ratio optimization model to adjust the formula ratio to obtain the optimal formulation effect, and weighing proanthocyanidin extract, chitosan, vitamin C, vitamin E, collagen, hyaluronic acid, lotus leaf extract, astragalus polysaccharide, casein phosphopeptide and D-mannitol according to the formula ratio; Step 2: Prepare a solution of each component, mix the prepared solution together, and stir it for 2 to 4 minutes at a temperature of ℃ to obtain a proanthocyanidin complex solution; Step 3, drying the proanthocyanidin complex solution by low-temperature vacuum drying, in the pre-freezing stage, quickly freezing the proanthocyanidin complex solution at a low temperature of -40 to -50°C for 3 to 5 hours, until the water in the solution is completely solidified into ice crystals; in the vacuum drying stage, the pressure is 10 to 13 Pa, the temperature is below 0°C, and the drying time is 4 to 6 hours to obtain a proanthocyanidin complex powder; Step 4: The dried proanthocyanidin complex powder is measured by a capsule filling machine and divided into medicinal hard capsules to obtain the proanthocyanidin complex hard capsules, each hard capsule containing 0.5 g of the substance.

6. The process for preparing the proanthocyanidin complex hard capsule for beauty and health food according to claim 5, characterized in that: The solution preparation process comprises the following steps: dissolving proanthocyanidin extract and lotus leaf extract in edible alcohol with a concentration of 2% to prepare proanthocyanidin aqueous solution and lotus leaf extract aqueous solution; dissolving chitosan and vitamin E in an aqueous acid solution with a concentration of less than 5% to prepare chitosan aqueous solution and vitamin E aqueous solution; and mixing vitamin C, astragalus polysaccharide and D-mannitol with water, stirring until completely dissolved, to obtain vitamin C solution, astragalus polysaccharide solution and D-mannitol solution.

7. The process for preparing the proanthocyanidin complex hard capsule for beauty and health food according to claim 5, characterized in that: The components of the hard capsule include 30-50 parts of gelatin, 0.1-1.5 parts of glycerol, 1.5-4 parts of starch, 0.2-2 parts of magnesium stearate and 5-15 parts of water; the gelatin has biocompatibility and degradability, and can be safely dissolved and absorbed in the human body; the glycerol acts as a plasticizer to increase the softness and plasticity of the gelatin and maintain the humidity of the hard capsule; the magnesium stearate acts as a lubricant to reduce the friction and adhesion of the hard capsule during the preparation process; the starch adjusts the hardness and brittleness of the hard capsule. When the starch content is 12-15%, the hardness range of the hard capsule is 18-25N, when the starch content is 8-11%, the hardness range of the hard capsule is 11-17N, and when the starch content is 3-7%, the hardness range of the hard capsule is 5-10N.

8. An application of a proanthocyanidin complex hard capsule for beauty and health food, characterized in that: The proanthocyanidin complex hard capsule for beauty and skin care health food according to claims 1-4 is characterized in that: the proanthocyanidin complex hard capsule utilizes the antioxidant property of proanthocyanidins to remove excess free radicals in the body and reduce cell damage; the chitosan is used as a stabilizer and carrier to form a mixture with proanthocyanidins to enhance moisture retention, film-forming property and biocompatibility; the D-mannitol is used as a filler and sweetener to improve the taste and texture of the capsule; the hyaluronic acid absorbs and retains a large amount of water to keep the skin hydrated and increase the beauty effect.

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