Formula composition applied to antioxidant and anti-aging food and production method of formula composition

Abstract

The invention discloses a formula composition which is prepared from the following components in parts by weight: 100 to 400 parts by weight of grape seed procyanidine, 100 to 300 parts by weight of pomegranate polyphenol, 10 to 100 parts by weight of ergothioneine, 50 to 200 parts by weight of catechin and 100 to 400 parts by weight of astragalus polysaccharide. According to the invention, cell-level anti-aging is taken as a core logic, five active ingredients of grape seed procyanidine, pomegranate polyphenol, ergothioneine, catechin and astragalus polysaccharide are scientifically compounded, a multi-dimensional anti-oxidation defense system is constructed, and multiple effects of scavenging free radicals, repairing cell damage, delaying an aging process and the like can be realized.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional foods, and in particular to a food formula composition for anti-oxidation and anti-aging and a production method thereof. Background Art

[0002] Aging and the occurrence of chronic diseases (such as cancer, cardiovascular and cerebrovascular diseases, and diabetes) are closely related to the excessive accumulation of free radicals in the body. Free radicals can oxidize lipids, DNA, and proteins, leading to cell membrane damage, gene mutations, and tissue dysfunction. For example, malondialdehyde (MDA), a lipid peroxidation product, accelerates the formation of wrinkles and age spots, while inflammatory responses triggered by oxidative stress exacerbate the cellular aging process.

[0003] Currently, most antioxidant products on the market are based on a single ingredient (such as vitamins C and E), making it difficult to build a multi-dimensional defense system. Furthermore, some synthetic antioxidants pose safety risks, while the lack of systematic design in the formulation of natural ingredients limits their antioxidant effectiveness. Summary of the Invention

[0004] This invention takes "cellular-level anti-aging" as its core logic and scientifically compounds the five active ingredients of grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, and astragalus polysaccharides to construct a multi-dimensional antioxidant defense system, which can achieve multiple functions such as scavenging free radicals, repairing cell damage, and delaying the aging process.

[0005] In a first aspect, the present invention provides a formula composition comprising, by weight, 100-400 parts by weight of grape seed proanthocyanidins, 100-300 parts by weight of pomegranate polyphenols, 10-100 parts by weight of ergothioneine, 50-200 parts by weight of catechins, and 100-400 parts by weight of astragalus polysaccharides.

[0006] In a preferred embodiment, the formula composition comprises, by weight, 120-300 parts by weight of grape seed proanthocyanidins, 120-250 parts by weight of pomegranate polyphenols, 20-80 parts by weight of ergothioneine, 50-150 parts by weight of catechins, and 200-330 parts by weight of astragalus polysaccharides.

[0007] In a preferred embodiment, the formula composition comprises, by weight, 150 parts by weight of grape seed proanthocyanidins, 200 parts by weight of pomegranate polyphenols, 20 parts by weight of ergothioneine, 100 parts by weight of catechins, and 330 parts by weight of astragalus polysaccharides. This combination has the best oxidative activity against SOD, GPx, and hydrogen peroxide.

[0008] In a preferred embodiment, the formula composition comprises, by weight, 120 parts by weight of grape seed proanthocyanidins, 250 parts by weight of pomegranate polyphenols, 80 parts by weight of ergothioneine, 50 parts by weight of catechins, and 300 parts by weight of astragalus polysaccharides. This combination has strong oxidative activity against SOD, GPx, and hydrogen peroxide.

[0009] In some embodiments, the grape seed proanthocyanidins are derived from natural grape extract. Grape seed powder has antioxidant, anti-aging, cardiovascular protection, cholesterol and triglyceride lowering, atherosclerosis prevention, eye protection, collagen synthesis promotion, skin elasticity and radiance increase, and wrinkle reduction effects.

[0010] In some embodiments, the pomegranate polyphenols are derived from pomegranate extract. Pomegranate polyphenols have antioxidant and anti-aging effects, preventive and therapeutic effects on neurodegenerative diseases (such as Alzheimer's disease and Parkinson's disease), and can also protect cardiovascular health and fight cancer.

[0011] In some embodiments, the ergothioneine is derived from a natural edible fungus extract. Ergothioneine is a natural antioxidant that reduces the risk of oxidative stress-related diseases, such as cardiovascular disease, neurodegenerative diseases, etc. In skin cells, ergothioneine can resist ultraviolet damage, reduce the damage to cells by external factors such as pollution, delay skin aging, reduce the damage of neurotoxins to nerve cells, and has some auxiliary therapeutic effects in diseases such as Parkinson's and Alzheimer's.

[0012] In some embodiments, the catechins include gallocatechin and EGCG gallate. Catechins have antioxidant, cholesterol-lowering, low-density glycerol (LDT)-lowering, atherosclerotic, anti-thrombotic, wrinkle-reducing, and neuroprotective effects, and can prevent Alzheimer's and Parkinson's diseases.

[0013] In some embodiments, the astragalus polysaccharide is derived from an astragalus extract. Astragalus can increase telomere length and has antioxidant, anti-aging, immunity-enhancing, and cardiovascular protection effects.

[0014] In some embodiments, an excipient may be further added to the formula composition, and the excipient is selected from isomalt, hydroxypropyl methylcellulose, and magnesium stearate.

[0015] In a second aspect, the present invention provides a method for producing the formula composition, comprising mixing grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, astragalus polysaccharides, and excipients to obtain the formula composition.

[0016] In a third aspect, the present invention provides the use of the formula composition in the preparation of antioxidant and anti-aging foods.

[0017] In some embodiments, the formulated composition increases the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase.

[0018] In some embodiments, the formula composition can scavenge free radicals in cells, such as superoxide anion radicals, hydroxyl radicals, etc., to protect cells from oxidative damage; it can play a protective role at the cellular level and maintain the normal function and structural integrity of cells.

[0019] In some embodiments, the antioxidant and anti-aging food can be in any food form, for example, a tablet.

[0020] In a fourth aspect, the present invention provides a method for producing an antioxidant and anti-aging food, comprising: Step 1: Grind grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, astragalus polysaccharides, and excipient raw materials separately, pass through a 40-mesh sieve, and mix the raw material powders according to a weight ratio to obtain a mixed powder; Step 2: add 85% ethanol solution to the mixed powder, granulate, dry, and shape the granules; add magnesium stearate thereto; mix and compress the granules into tablets; and package the granules.

[0021] In some embodiments, in step 2, the weight ratio of the mixed powder to 85% ethanol is (10-20):1.

[0022] In some embodiments, in step 2, the drying is performed at 50-60° C. until the moisture content of the particles is ≤5%.

[0023] In some embodiments, in step 2, the weight ratio of magnesium stearate to particles is 1:(100-1000).

[0024] In some embodiments, in step 2, the hardness of the tablet obtained by tableting is ≥6.0 kgf / cm 2 .

[0025] In some embodiments, step 1 and step 2 are performed in a Class 100,000 clean area.

[0026] Technical Effects

[0027] Natural ingredients such as grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, and astragalus polysaccharides all have significant antioxidant activity, but the target of action of a single ingredient is limited.

[0028] This invention uses "cellular anti-aging" as its core principle, scientifically combining five active ingredients: ergothioneine, proanthocyanidins, pomegranate polyphenols, catechins, and astragalus polysaccharides. Ergothioneine specifically scavenges hydroxyl free radicals, blocking the initial oxidation step; proanthocyanidins and pomegranate polyphenols inhibit the lipid peroxidation chain reaction; catechins repair oxidative DNA damage; and astragalus polysaccharides activate the Nrf2 antioxidant pathway, achieving a multi-target synergistic effect.

[0029] This invention provides a precisely formulated combination to optimize the synergistic effects of the active ingredients. Through experiments with different ratios, the optimal combination of ingredients was found to enhance antioxidant enzyme activity. Increases in superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase, with no adverse effects on human health, indicate that the test sample possesses antioxidant and anti-aging properties. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a process flow chart for preparing antioxidant and anti-aging food in a preferred embodiment of the present invention. DETAILED DESCRIPTION

[0031] The following describes several preferred embodiments of the present invention to make its technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the scope of protection of the present invention is not limited to the embodiments mentioned herein.

[0032] Examples 1-6

[0033] Examples 1-6 respectively provide a method for preparing a food comprising a formula composition for use in antioxidant and anti-aging foods, and the process flow is as follows: Figure 1 As shown, the method comprises: crushing grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, and astragalus polysaccharides raw materials respectively, passing through a 40-mesh sieve, and mixing the raw material powders according to the weight ratio shown in Table 1 to obtain a mixed powder; 84 mg of 85% ethanol solution was added to the mixed powder, granulated, dried, and sized, and 16 mg of magnesium stearate was added thereto. After mixing, the mixture was tableted and packaged.

[0034] Experimental Example 1

[0035] The activity of the formulated compositions obtained in Examples 1-6 was tested.

[0036] Superoxide dismutase (SOD) is a widely present antioxidant metalloenzyme in organisms that catalyzes the dismutation of superoxide anion radicals, thereby protecting cells from oxidative stress. Therefore, accurate detection of SOD activity is crucial for assessing an organism's antioxidant capacity and studying related diseases.

[0037] The test method for SOD is as follows: 1. Sample Processing Collect approximately 2 mL of blood from the animal vein; centrifuge for 10 minutes (3000 g) to remove serum; add 1 mL of distilled water to resuspend and lyse red blood cells, and incubate at 4°C for 2 hours.

[0038] 2. Removal of hemoglobin

[0039] Take 1.88 mL of lysate and add 0.8 mL of chloroform-ethanol mixture (3:5 v / v) and 0.3 mL of water; this step is used to precipitate hemoglobin. Then centrifuge again (3000g, 10 minutes) and collect the supernatant for enzyme activity determination.

[0040] 3. Principle of SOD activity assay

[0041] Using riboflavin to generate superoxide anions (O2 - ), which can reduce nitro blue tetrazolium (NBT) to form purple formazan. The role of SOD is to remove O2 - , thereby inhibiting the reduction reaction of NBT.

[0042] The reaction system includes: 10 -2 M Methionine 2×10 -5 M Riboflavin 1.67×10 -4 M NBT 2×10 -5 M KCN Buffer: 0.05 M phosphate buffer, pH 7.8 4. Illumination and Measurement Add 5 µL of supernatant to 2 mL of reaction system; irradiate with 365 nm UV light for 2 minutes; and measure the absorbance change (OD) at 560 nm.

[0043] Definition of SOD activity unit: the amount of enzyme that inhibits the NBT reduction reaction by 50%.

[0044] 5. Quantitative calculation

[0045] Purified human SOD (erythrocuprein) was used to construct a standard curve.

[0046] The inhibition rate was linearly related to the enzyme activity (within 40% inhibition rate).

[0047] Activity unit conversion formula: SOD activity unit = inhibition rate (%) 6.34 Glutathione peroxidase (GPx) is an antioxidant enzyme that participates in the removal of reactive oxygen species such as peroxides in the body.

[0048] GPx, test method (based on an improvement of Gunzler's method): 1. Sample processing: 1 mL of blood was collected and centrifuged at 3000 g for 10 minutes to remove serum; red blood cells were washed three times with 0.9% NaCl.

[0049] Add 1 mL of distilled water to lyse → Freeze (-70°C) → Thaw quickly.

[0050] 2. Lysis solution treatment: Dilute to a hemoglobin concentration of 5 g / 100 mL; mix with an equal volume of oxidation mixture (containing 4×10 -3 M potassium ferrocyanide, 2×10 -3 M KCN, 0.1 M phosphate buffer, pH 7.0).

[0051] 3. Enzyme activity assay reaction system (total volume 3 mL): 2×10 -4 M NADPH 10 -3 M Reduced glutathione 10 -3 M t-Butyl peroxide (instead of H2O2) 3 IU yeast glutathione reductase 100 μL of the above treated lysate Incubate in 0.05 M pH 7.0 phosphate buffer at 25°C 4. Detection method: The oxidation rate of NADPH was monitored spectrophotometrically at 340 nm using a molar extinction coefficient of 6.22 × 10³ M. -1 cm -1 .

[0052] Blank control: Hemoglobin samples were used that had been GPx-depleted using DEAE Sephadex A-50.

[0053] Enzyme activity calculation formula: GPx activity = (ΔOD / min sample ΔOD / min (blank) × 193 The unit is: μmol NADPH consumption / min / g hemoglobin.

[0054] Catalase is a widely present oxidoreductase in living organisms, efficiently catalyzing the decomposition of hydrogen peroxide (H2O2) into water and oxygen. It serves as a key line of defense for cells against oxidative damage and plays a vital role in maintaining metabolic homeostasis. It is commonly found in the liver, red blood cells, and plant tissues.

[0055] Catalase, test method: 1. Sample processing: Take a portion of the sample from the red blood cell lysate and dilute it 100-fold; take 10 μL of the dilution and add it to 5 mL of H2O2 solution (concentration is 2.1×10 -5 M, buffer was 0.01 M phosphate, pH 7.8).

[0056] Incubation conditions: Incubate at 25°C for 30 minutes.

[0057] 2. Determination of residual H2O2: The remaining H2O2 was detected by luminol reaction catalyzed by horseradish peroxidase (HRP); the reaction system (total volume 3 mL) included: 10 µg HRP 10- 4 M EDTA 10 -5 M luminol 10 -5 M phosphate buffer (pH 7.8) After injecting 1 mL of the incubation mixture, measure: maximum luminescence intensity (Imax), total luminescence (L) 3. Quantitative calculation: A standard curve was established using bovine liver catalase.

[0058] Catalytic enzyme activity (µg / mL blood) can be calculated using the following formula: Use Imax: Catalase (µg / mL) = (49 logImax)×525+50 Or use the total luminescence L: Catalase (µg / mL) = (log45-logL) × 825 + 50 The blank control was performed by fine-tuning the H2O2 concentration to keep Imax+L constant.

[0059] Baseline values: superoxide dismutase (SOD) -152 U / ml blood, glutathione peroxidase (GPx) 0.73 μmol NADPH / min / ml blood, catalase 510 μg / ml blood. The test results are shown in Table 1.

[0060] Table 1

[0061] It can be seen that, with the same total weight (800 mg), different ratios of the five active ingredients have varying degrees of effect on SOD, GPx, and catalase, but all achieve an improvement of approximately 30% or more in antioxidant and anti-aging performance, with some even reaching as high as 56%. In Example 2, the ratio of 20 parts by weight of ergothioneine, 150 parts by weight of grape seed proanthocyanidins, 200 parts by weight of pomegranate polyphenols, 100 parts by weight of catechins, and 330 parts by weight of astragalus polysaccharides achieved the best catalase activity, SOD, and GPx.

[0062] Comparative Examples 1-5

[0063] The same test method as above was used to determine the effects of grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, and astragalus polysaccharides on SOD, GPx, and catalase when used alone. The results are shown in Table 2.

[0064] Table 2

[0065] As can be seen from Table 2, although the use of a single variety also has a certain degree of antioxidant activity and improves the effects on SOD, GPx and catalase, its antioxidant performance is significantly lower than the antioxidant and anti-aging food formula composition of Examples 1-6.

[0066] The preferred embodiments of the present invention have been described in detail above. It should be understood that numerous modifications and variations based on the concepts of the present invention are possible without inventive effort by those skilled in the art. Therefore, any technical solution that can be derived by one skilled in the art through logical analysis, reasoning, or limited experimentation based on the concepts of the present invention and the prior art should be within the scope of protection defined by the claims.

Claims

1. A formula composition, comprising, by weight, 100-400 parts by weight of grape seed proanthocyanidins, 100-300 parts by weight of pomegranate polyphenols, 10-100 parts by weight of ergothioneine, 50-200 parts by weight of catechins, and 100-400 parts by weight of astragalus polysaccharides.

2. The formulation composition according to claim 1, wherein In parts by weight, the formula composition comprises 120-300 parts by weight of grape seed proanthocyanidins, 120-250 parts by weight of pomegranate polyphenols, 20-80 parts by weight of ergothioneine, 50-150 parts by weight of catechins, and 200-330 parts by weight of astragalus polysaccharides.

3. The formulation composition according to claim 1, wherein In parts by weight, the formula composition comprises 150 parts by weight of grape seed proanthocyanidins, 200 parts by weight of pomegranate polyphenols, 20 parts by weight of ergothioneine, 100 parts by weight of catechins, and 330 parts by weight of astragalus polysaccharides.

4. The formulation composition according to claim 1, wherein In parts by weight, the formula composition comprises 120 parts by weight of grape seed proanthocyanidins, 250 parts by weight of pomegranate polyphenols, 80 parts by weight of ergothioneine, 50 parts by weight of catechins, and 300 parts by weight of astragalus polysaccharides.

5. The formulation composition according to any one of claims 1 to 4, wherein The formula composition comprises an excipient, and the excipient is selected from isomalt, hydroxypropyl methylcellulose, and magnesium stearate.

6. A method for producing the formula composition according to any one of claims 1 to 4, comprising mixing grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, and astragalus polysaccharides to obtain the formula composition.

7. Use of the formula composition according to any one of claims 1 to 5 in the preparation of antioxidant and anti-aging foods.

8. The use according to claim 7, wherein: The formula composition increases the activities of superoxide dismutase, glutathione peroxidase and catalase.

9. A method for producing an antioxidant and anti-aging food, comprising: Step 1: Grind grape seed proanthocyanidins, pomegranate polyphenols, ergothioneine, catechins, astragalus polysaccharides, and excipient raw materials separately, pass through a 40-mesh sieve, and mix the raw material powders according to a weight ratio to obtain a mixed powder; Step 2: Add 85% ethanol solution to the mixed powder, granulate, dry, and shape the granules; add magnesium stearate thereto; mix and compress the mixture into tablets; and package the mixture.

10. The production method according to claim 9, wherein In step 2, the weight ratio of magnesium stearate to the particles is 1:(100-1000).

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