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Tomato ubiquitination E3 ligase gene SlCHIP1, application and gene SlCHIP2

A technology of ubiquitination and ligase, applied in the direction of ligase, application, genetic engineering, etc., can solve problems such as unknown function, and achieve the effect of improving high temperature resistance

Active Publication Date: 2020-06-09
LISHUI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that AtCHIP plays an important role in Arabidopsis resistance to high temperature stress (PLoS Genet.2014,10(6):e1004118), while the homologous gene of CHIP in tomato has not been cloned, and its function is unknown

Method used

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  • Tomato ubiquitination E3 ligase gene SlCHIP1, application and gene SlCHIP2
  • Tomato ubiquitination E3 ligase gene SlCHIP1, application and gene SlCHIP2
  • Tomato ubiquitination E3 ligase gene SlCHIP1, application and gene SlCHIP2

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Embodiment Construction

[0019] The nucleotide sequence of the tomato ubiquitinated E3 ligase gene SlCHIP1 is as follows:

[0020]ATGGCACCAATCGTGGGTTCAAAGCAAGCGGAACAACTAAAGC AAGATGGGAACAATTATTTTCAGAAGAATCGGTTTGGCGCTGCCATTG ATGCTTATACCGAGGCTATTACTTTGTGCCCTAATGTTCCGATATATTGGACAAATCGTGCTCTATGTCATCGGAGGCGGAATGACTGGAGAAGAGT GGAGGAAGATTGCAGGAGGGCGATTCAGCTCGATCATAATTCTGTAA AGGCCCACTATTATCTTGGTCTTGCATTGCTACAAAAGGAAGCATTTG GTGAAGGTGTGAGAGAATTGGAAAAGGCATTAGACCTTGGAAGAGG TGTGAATCCAGGAAGATATATTGTTGGAGAGATCTGGGAAGAGCTTGC TAAAGCAAAGTACATGGAGTGGGAGCATGAATCTACAAGGCGCTCCT GGGAGCTTCAGAACTTGAAAGAATCATGTGAGTCAGCTCTCAAGGAG AGACATATGCTTGACAGTTCTCAGACAGAAGGGTTCAAAGATGAAAA ATCAACGGCGCTTTTTAAGCAACTGAAAGCTCTAGGTAAGGTTTTCGT GAAAGCTGCAGCAGATGATACTCCAACTGAGGTTCCCGATTACTTGTG TTGTAAAATTAGTCTTGATATTTTTCGTGACCCTGTTATTACTCCGAGTGGGTTTACATATGAGCGGGCTGTGATCCTTGAGCATTTGCAAAAGGTG GGAAAATTTGATCCAATCACAAGAGAACCACTTTTGCCGTCCCAGTT GGTGCCAAACCTGGCCATAAAAGAATCTGTACGGGCGTTTTTGGATAGGCATGGCTGGGCATATAGGATATAG。

[0021] The nucleotide sequence of the t...

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Abstract

The invention relates to the technical field of biology, in particular to a tomato ubiquitination E3 ligase gene SlCHIP1, application and a gene SlCHIP2. The nucleotide sequences of the tomato ubiquitination E3 ligase genes SlCHIP1 and SlCHIP2 are cloned, the homology of the two genes, the evolutionary relationship with homologous genes of other species, and the effect and mechanism in high-temperature resistance of tomatoes are studied and discussed, and a new gene resource and a possible molecular marker are provided for high-temperature-resistant breeding and seed selection of the tomatoesand other species.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of tomato ubiquitinated E3 ligase genes SlCHIP1, SlCHIP2 and SlCHIP1. Background technique [0002] With the development and progress of my country's agriculture, the vegetable planting industry is becoming more and more industrialized. However, it is the large-scale planting of vegetables that faces more and more biological or abiotic disasters, and the advent of disasters often causes huge economic losses. Especially global climate warming, the temperature rises gradually, has seriously affected the growth and reproduction of crops. Tomato, as a crop grown in summer, is often faced with the test of high temperature. How to fundamentally solve the problem of tomato's resistance to high temperature is of great significance. [0003] Ubiquitination is one of the most common post-translational modifications in the proteome. It is catalyzed by three enzymes (E1, E2 and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/52C12N15/82A01H5/00A01H6/82
CPCC12N9/93C12Y603/02019C12N15/8271
Inventor 张燕徐丽夏更寿赖小东杨思晴傅伟红任欢袁静雅石澄琛金欢淳
Owner LISHUI UNIV
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