Composite repair material for bridging defect nerves and stent made of composite repair material

A composite repair and composite material technology, applied in the composition, preparation and application of composite repair materials and their scaffolds, can solve problems such as lack, achieve the effects of preventing fibrous scars, reducing the difficulty of surgical techniques, and improving safety

Active Publication Date: 2016-01-13
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported nerve repair strategies lack an ideal nerve repair material, which can not only provide the best physical, chemical and biological microenvironment for nerve regeneration, but also ensure that the operation is simple and fast, and there is no recurrent tear after surgery. problems, to achieve the multiple purposes of fast, safe and good prognosis of nerve anastomosis

Method used

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  • Composite repair material for bridging defect nerves and stent made of composite repair material
  • Composite repair material for bridging defect nerves and stent made of composite repair material
  • Composite repair material for bridging defect nerves and stent made of composite repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Animal Modeling and Evaluation of Nerve Injury

[0029] a. Canine sciatic nerve injury model

[0030] A young Beagle dog was selected and anesthetized by intraperitoneal injection of sodium pentobarbital (30 mg / kg). A longitudinal incision was made at the posterior part of the right thigh, the skin was cut, and the muscle was separated to expose the right sciatic nerve. The sciatic nerve was cut off with a double-sided blade.

[0031] b. Canine spinal cord transection model

[0032] Young Beagle dogs were selected and anesthetized by intraperitoneal injection of pentobarbital sodium (30mg / kg). The skin was incised under sterile conditions, the muscles were separated, and the T9 and T10 spinal lamina were excised, and half-transected at the middle of the T9 lamina The spinal cord (located at the junction of T9 and T10 spinal cord segments), and a 2mm spinal cord tissue block on the right half was excised, and the residual nerve fibers in the injury cavity wer...

Embodiment 2

[0033] The preparation of embodiment 2 different restorative materials

[0034] a. Silk fibroin conduit

[0035] Dissolve the silk in the ternary system of calcium chloride, ethanol and water (the molar ratio is calcium chloride: ethanol: water = 1: 2: 8), heat treatment at 80°C for 5 hours, and fill the dissolved mixed solution into the fiber The plain film bag is dialyzed with distilled water to obtain a silk fibroin solution, which is poured into a mould, dried and then molded. Put the preliminarily formed silk fibroin catheter into methanol and sodium hydroxide solution with a volume ratio of 50:50 for 2 hours, and then wash it successively with 1mol / L sodium hydroxide solution, 50mmol / L phosphate buffer and distilled water , to obtain silk fibroin conduits.

[0036] b. Collagen Scaffold

[0037]Prepare a collagen solution with a concentration of 25 mg / ml, pour it into a mold, and freeze it at -15°C for 80 minutes, then put it in a freeze dryer for 25 minutes in a vacuu...

Embodiment 3

[0043] Embodiment 3 Preparation of silk fibroin-collagen-polymer nerve repair material and scaffold thereof

[0044] a. Preparation of silk fibroin-collagen-PLGA composite scaffold:

[0045] (1) prepare the PLGA solution that concentration is 0.05g / ml with volatile organic solvent, pour into mold and dry naturally, obtain the PLGA film that the thickness that obtains appearance is T-shaped is 50um;

[0046] (2) Prepare a collagen solution with a concentration of 5 mg / ml, pour it on the glass slide of the PLGA membrane, and let it dry naturally to obtain the attached collagen solution. figure 2 Type A collagen-PLGA bilayer membrane;

[0047] (3) silk is dissolved in the ternary system of calcium chloride, ethanol and water (molar ratio is calcium chloride: ethanol: water=1: 2: 8), heat treatment at 80 ℃ for 5 hours, the mixed solution of dissolving Put it into a cellulose film bag, and dialyze it with distilled water to obtain a silk fibroin solution. Use three-dimensional p...

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Abstract

The invention provides a composite repair material which facilitates surgical operation and realizes quick defect nerve bridging and composition, preparation and application of a stent made of the composite repair material. The composite repair material consists of a fibroin layer, a collagen layer and a high-molecular polymer layer which are sequentially arranged, and during application, the composite repair material is folded or wound to form the nerve bridging stent taking the fibroin layer as the inner layer, the collagen layer as the middle layer and the high-molecular polymer as the outer layer. The composite repair material and the stent made of the composite repair material adopt the layered arrangement and a specific three-dimensional design to bring the advantages of various repair materials into full play, and meanwhile, make up for the deficiencies of the various repair materials. During surgical operation, only suturing or bonding anastomosis of epineurium is required but not suturing of perineurium, so that the operation time of a defect nerve bridging operation is greatly shortened, the operation technical difficulty is lowered, and the security is improved.

Description

technical field [0001] The invention relates to a repairing material for fast bridging of nerves, in particular to the composition, preparation and application of a composite repairing material for fast bridging of defective nerves which is convenient for operation and realizes fast bridging of nerve defects. Background technique [0002] Clinically, central and peripheral nerve injuries caused by trauma are very common, and long-distance nerve defects cannot usually be compensated by end-to-end suture. The most commonly used transplant material in clinical practice is autologous nerve, but the diameter of the donor is very small, and the number that can be excised is very limited, which is difficult to meet the clinical needs. Therefore, it is necessary to rely on repair materials or graft stents to bridge the repair. Finding and developing more suitable repair materials or grafting scaffolds is the key to solve the repair of defective nerves. At present, autologous nerves...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/50A61L27/54
Inventor 赵应征鲁翠涛肖健虞希冲徐荷林林倩田福荣毛凯丽夏维婷
Owner WENZHOU MEDICAL UNIV
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