Preparing method and application of human mesenchymal stem cell-sourced exosome beautifying preparation

A technology of stem cells and exosomes, applied in the field of biomedicine, can solve the problems of poor individual targeting, serious product homogeneity, and single active ingredients, so as to achieve the rejuvenation of skin cells, promote regeneration and repair, and restore age. trace effect

Inactive Publication Date: 2018-11-02
成都赛伊泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the cosmetic preparations on the market have a single active ingredient, are n...

Method used

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  • Preparing method and application of human mesenchymal stem cell-sourced exosome beautifying preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1: Preparation of human mesenchymal stem cell exosomes

[0026] 1. Culture of Mesenchymal Stem Cells

[0027] a) Soak the fresh umbilical cord in PBS buffer solution containing 5% penicillin-streptomycin, and rinse it repeatedly for 3-5 times until there is no blood and other impurities;

[0028] b) Cut the umbilical cord into small pieces of 3-4cm, and cut it along the umbilical cord. It can be observed with the naked eye that it contains three blood vessels, two of which are at both ends of the vein. The outer mold of the umbilical cord is peeled off along one of the arteries, and the arteries are torn off at this time Below, the viscous substance between the artery and the vein can be seen, that is, Wharton glue, which is torn off little by little and placed on a petri dish dripped with a small amount of PBS;

[0029] c) Separate the Wharton glue between the other artery and the vein in the same way, finally peel off the vein, and separate the Wharton glue l...

Embodiment 2

[0044] Example 2: Preparation of exosome cosmetic preparations derived from human mesenchymal stem cells

[0045] 1. Formula

[0046] 9.2g of human mesenchymal stem cell exosomes, 0.8g of mannitol, 42mg of oligopeptide-32, 130mg of tetrapeptide-4, 70mg of palmitoyl tripeptide-1, 80mg of palmitoyl pentapeptide-4

[0047] In the cosmetic preparation of the present invention, human mesenchymal stem cell exosomes are used as the main component, and an appropriate amount of mannitol is used as an excipient. Adding mannitol to the freeze-dried product can have a good effect, changing the quality of the freeze-dried product. At the same time, it can also play a certain protective role for polypeptide products, which is conducive to the stability of products. And the dosage of mannitol in the present invention can ignore its own pharmacological effects.

[0048] The oligopeptide-32, which has better skin permeability and is more easily absorbed, is added to the cosmetic preparation ...

Embodiment 3

[0052] Example 3: Preparation of exosome cosmetic preparations derived from human mesenchymal stem cells

[0053] 1. Formula

[0054] 10g human mesenchymal stem cell exosomes, 1g mannitol, 50mg oligopeptide-32, 200mg tetrapeptide-4, 100mg palmitoyl tripeptide-1, 100mg palmitoyl pentapeptide-4

[0055] 2. Preparation method

[0056] Take 10 g of human mesenchymal stem cell exosomes (ultracentrifugation) purified in Example 1, add 1 g of mannitol, 50 mg of oligopeptide-32, 200 mg of tetrapeptide-4, and 100 mg of palmitoyl tripeptide-1 100 mg of palmitoyl pentapeptide-4, after mixing, filter through 0.45 μm and 0.22 μm microporous membranes successively, then freeze and vacuum dry for 30 hours at a temperature of -65°C and a freezing pressure of 10 Pa in a vacuum. Obtain the cosmetic anti-aging exosome cosmetic preparation derived from human mesenchymal stem cells, and store it at room temperature.

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Abstract

The invention discloses a preparing method and application of a human mesenchymal stem cell-sourced exosome beautifying preparation. The beautifying preparation comprises 92-108 parts of human umbilical cord mesenchymal stem cell exosome, 8-13 parts of mannitol, 0.42-0.55 part of oligopeptide-32, 1.3-2.5 parts of tetrapeptide-4, 0.7-1.3 parts of palmitoyl tripeptide-1 and 0.8-1.2 parts of palmitoyl pentapeptide-4. The preparation method includes: evenly mixing and stirring the human mesenchymal stem cell exosome, the mannitol, the oligopeptide-32, the tetrapeptide-4, the palmitoyl tripeptide-1and the palmitoyl pentapeptide-4 according to the weight, filtering through a filter membrane, performing split charging, and performing freeze drying to obtain the human mesenchymal stem cell-sourced exosome beautifying preparation. The exosome beautifying preparation can be externally applied to the epidermal layer or guided into an epidermis deep layer and the corium layer through a microneedle, a rolling needle or a hydro-lifting needle. The ImmuReg technology is utilized to bring the optimal repairing effect of freeze-dried powder into play, the skin immune state can be precisely adjusted, and accordingly aged cells can be removed, and skin cells can be rejuvenated.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method and application of an exosome cosmetic preparation derived from human mesenchymal stem cells. Background technique [0002] Exosomes are nanoscale lipid bilayer vesicles released by exocytosis to the outside of cells, and can exist in various types of extracellular body fluids, such as blood, urine, amniotic fluid, saliva, cerebrospinal fluid, and even breast milk. Studies have found that exosomes contain cell-specific proteins, lipids and nucleic acids, which can be transmitted to other cells as signaling molecules, thereby changing the functions of other cells. For example, exosomes participate in the regulation of basic physiological processes such as neuronal communication, antigen presentation, immune response, organ development and reproduction, and also participate in the regulation of cancer progression, cardiovascular disease, inflammation, neurodegenerati...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/98A61K8/64A61Q19/00A61Q19/02A61Q19/08C12N5/0775
CPCA61K8/99A61K8/64A61K8/982A61Q19/00A61Q19/02A61Q19/08C12N5/0665
Inventor 雷菁刘梦梅刘慧莹吴桐
Owner 成都赛伊泰生物科技有限公司
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