Chitosan-based styptic sponge and preparation method thereof

A hemostatic sponge and chitosan-based technology, applied in medical science, bandages, absorbent pads, etc., can solve the problems of poor thrombin stability, easy inactivation, and restrictions on the application of hemostatic sponges, and achieve good coagulation healing effect and excellent properties The effect of stabilizing and enhancing the healing effect of blood coagulation

Inactive Publication Date: 2012-07-04
WUHAN GENERAL HOSPITAL OF GUANGZHOU MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it still has the disadvantages of poor thrombin stability and easy inactivation, which limits the application of the hemostatic sponge

Method used

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  • Chitosan-based styptic sponge and preparation method thereof
  • Chitosan-based styptic sponge and preparation method thereof
  • Chitosan-based styptic sponge and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Chitosan was dissolved in distilled water to obtain a chitosan aqueous solution with a mass concentration of 0.5%; thrombin was dissolved in 0.1mol L of pH 7.0 -1 Phosphate buffer to obtain a thrombin solution with a mass concentration of 0.0015%, and then mix the two at a volume ratio of 3:1 to obtain 400 mL of a mixed solution with a weight ratio of chitosan and thrombin of 100:0.1. Under the condition of stirring, add 5g of glycerol and 10g of mannitol, and add 0.5mL of calcium chloride solution with a mass concentration of 5% dropwise, mix evenly, then pour into a mold with an appropriate shape, and place it for 2 hours until a transparent After gelation, freeze at -20°C for 24 hours, then vacuum freeze-dry at -40°C for 36 hours, mold, cut, and package. 60 The finished product is obtained after Co irradiation sterilization.

Embodiment 2

[0039] Chitosan was dissolved in distilled water to obtain a chitosan aqueous solution with a mass concentration of 2%; thrombin was dissolved in 0.1mol L of pH 7.0 -1 Phosphate buffer saline, obtain the thrombin solution that mass concentration is 0.06%, dissolve calcium chloride with distilled water, obtain the calcium chloride solution that mass concentration is 3%, then the three are mixed by volume ratio 3: 1: 0.1, Prepare chitosan, thrombin, Ca 2+410 mL of a mixed solution with a weight ratio of 100:1:1.8. Under the condition of stirring, add 4g glycerol and 20g mannitol, mix well. Then, under stirring, add 2.5 mL of glutaraldehyde solution with a volume concentration of 1% dropwise for cross-linking, then pour it into a mold with an appropriate shape, and let it stand for 2 hours. After forming a transparent gel, freeze it at -20°C for 24 hours. Then vacuum freeze-dry at -40°C for 36 hours, pour the mold, cut and package, 60 The finished product is obtained after Co ...

Embodiment 3

[0041] Chitosan was dissolved in distilled water to obtain a chitosan aqueous solution with a mass concentration of 2%; thrombin was dissolved in 0.1mol L of pH 7.0 -1 Phosphate buffer solution to obtain a thrombin solution with a mass concentration of 0.06%; dissolve the human-like collagen in distilled water to obtain a human-like collagen solution with a mass concentration of 2%, and then mix the three in a volume ratio of 3:1:60 Mix to prepare chitosan, thrombin, and human-like collagen in a weight ratio of 1:0.01:20 mixed solution 640mL. Under the condition of stirring, add 3.2g glycerol and 32g mannitol, mix well. Then, under stirring, add 2.5 mL of glutaraldehyde solution with a volume concentration of 0.1% dropwise for cross-linking, then pour it into a mold with an appropriate shape, and let it stand for 3 hours. After forming a transparent gel, freeze it at -20°C for 24 hours. Then vacuum freeze-dry at -20°C for 36 hours, pour the mold, cut and package, 60 The fini...

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Abstract

The invention relates to chitosan-based styptic sponge with a thrombin immobilization effect, and the chitosan-based styptic sponge is a porous sponge made from chitosan with the thrombin immobilization effect and hemostatic. The preparation method of the chitosan-based styptic sponge provided by the invention comprises the following steps: immobilizing thrombin with chitosan or carboxymethyl chitosan, and adding other styptics, cryoprotectants and crosslinking agents to prepare the porous styptic sponge, wherein the weight ratio of the chitosan or carboxymethyl chitosan to the thrombin is 100:(0.1-20); pre-freezing, lyophilizing in vacuum, casting, cutting, encapsulating and sterilizing to prepare the chitosan-based styptic sponge. According to the chitosan-based styptic sponge, the thrombin is immobilized through the chitosan or carboxymethyl chitosan, so that the stability and procoagulant activity of the thrombin can be improved, and the property of the prepared chitosan-based styptic sponge is more stable, the procoagulant and wound healing effects are remarkably improved, and the chitosan-based styptic sponge can be widely applied to wound or surgery hemostasis.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a chitosan-based hemostatic sponge with thrombin immobilization and a preparation method thereof, which is used for wound or operation hemostasis. Background technique [0002] Hemostasis is an important step in emergency medical treatment. Rapid hemostasis is required for surgical treatment of patients and sudden trauma in daily life. In the harsh war environment and emergency treatment of war and trauma in complex emergencies, it can be achieved Rapid and effective hemostasis is particularly important. [0003] Chitosan is a natural polycationic polysaccharide prepared by deacetylation of chitin, and carboxymethyl chitosan is a water-soluble derivative obtained after chemical modification of chitin. Both chitosan and carboxymethyl chitosan have multiple functions such as coagulation, antibacterial, promoting wound healing, and inhibiting scar formation. The multifunctional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/44A61L15/42
Inventor 刘辉浦金辉郭苗苗吴迪
Owner WUHAN GENERAL HOSPITAL OF GUANGZHOU MILITARY
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