The invention relates to the technical field of paint, and discloses an anti-doodling aqueous interior wall paint and a preparation method thereof. In the invention, an aqueous hydroxyl acrylate resin dispersion, a cellulose thickener, a bactericide, a mildew inhibitor, a wetting agent, a dispersant, a PH conditioning agent, a flatting powder, an antifoaming agent, titanium dioxide, a thickening agent and water are prepared to a component A according to the proportion, the component A and hydrophilic aliphatic polyisocyanate being as an component B are combined for usage, paint film surface can be effectively enclosed, thereby various kinds of spots can not penetrate in the surface of the paint film so that moisture cloth is easily used for wiping without any trace, the test proves that the anti-doodling aqueous interior wall paint has obvious anti-doodling effect. The prepared aqueous interior wall paint can meet the requirements of full hydration, the prepared paint contains no harmful solvent and has advantages of high environmental protection, compared with current anti-doodling paint, and the cost is low. In addition, the production technology is simple, construction is convenient, construction cycle is short, and the anti-doodling aqueous interior wall paint is beautiful and durable.