High efficiency experssino human glicentin-1 gene engineering bacteria and its construction method and use

A technology of glucagon and genetically engineered bacteria, which is applied in the field of bioengineering and can solve the problems of high synthesis cost, difficult purification, and high price
CN1587385AInactive Publication Date: 2005-03-02EAST CHINA NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA NORMAL UNIVERSITY
Publication Date
2005-03-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention belongs to the field of biological engineering technology. The gene engineering bacteria is colibacillus DH5-alpha, BL21(DE3) or BLR(DE3) carrying the recombinant plasmid of human glicentin-1 gene, i. e., GLP-1 gene. The construction process of the gene engineering bacteria includes connecting serially DNA sequence containing human glicentin-1 gene to form polymer, constituting expression vector and converting to colibacillus to obtain efficiently expressing human glicentin-1 gene engineering bacteria. With the gene engineering bacteria and through a three-step process of liquid culture, purification to produce human glicentin-1 fusion protein and preparation of human glicentin-1, human glicentin-1 product may be produced. The present invention has the advantages of high expression amount, less purification steps, high yield and low production cost.
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Description

technical field

[0001] The invention relates to a genetically engineered bacterium for highly expressing human glucagon-like peptide-1, its construction method and application, and belongs to the technical field of bioengineering. Background technique

[0002] Diabetes is a worldwide disease with a high incidence rate and poses a serious threat to human health. According to the latest report of the International Diabetes Institute (IDI) in 2003, there are 194 million people with diabetes worldwide. According to the current growth rate, by 2025, the number of patients will reach 333 million. There are more than 40 million diabetic patients in my country. In the early 1980s, the incidence rate of diabetes in my country was only 0.67%. Now the incidence rate in Beijing, Shanghai and other places has exceeded 10%, and this number is still increasing. According to statistics, the annual cost of treatment for ordinary diabetic patients is 3,726 yuan, whi...

Claims

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