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Methods for obtaining high-yielding oil palm plants

An oil palm, plant technology, applied in the field of obtaining high-yielding plants

Inactive Publication Date: 2013-03-27
SIME DARBY PLANTATION INTPROP SDN BHD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, given the many genetic and metabolic differences between rice, sunflower, and oil palm, and the highly specific nature of protein expression, rice and sunflower cannot be expected to improve oil palm yields by improving methods for obtaining and identifying high-yielding palms. These studies in the

Method used

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  • Methods for obtaining high-yielding oil palm plants
  • Methods for obtaining high-yielding oil palm plants
  • Methods for obtaining high-yielding oil palm plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Two-dimensional differential gel electrophoresis and protein identification

[0115] Target

[0116] Goals include identifying proteins that are differentially expressed in oil palm mesocarp tissue, including high- and low-yield traits, during fruit development.

[0117] method

[0118] Screening groups:

[0119] Two screening populations of oil palm plants were used, a high-yielding screening population and a low-yielding screening population, each comprising 3 individual palm plants. The above screening population was derived from the crossing of Serdang Avenue dura (at least 75% of Serdang Avenue dura) and AVROS pisifera (at least 75% of AVROS pisifera) to produce tenera progeny. More specifically, the high-yielding screen population is from a population of oil palm plants previously determined to be capable of producing relatively high yields of palm oil, specifically more than 10 tons of palm oil per hectare per year, thereby having a high-yielding phenoty...

Embodiment 2

[0171] Functions of 45 unique differentially expressed proteins

[0172] As shown in Table 1, the 45 unique differentially expressed proteins identified in Example 1 were annotated based on predicted molecular functions, involved pathways, and enzymatic classification. Surprisingly, only 3 of these 45 proteins were related to lipid metabolism function. These three proteins are phospholipase D, biotin carboxylase precursor and fructose bisphosphate aldolase. Furthermore, only 17 were successfully mapped to so-called KEGG pathways, ie according to the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway database, via http: / / www.genome.jp / keg / pathway.html (November 2010 most recently accessed) for pathways in which proteins function in the metabolism of carbohydrates, amino acids, lipids, nucleotides, energy, or secondary metabolism. These 17 proteins include the 3 lipid metabolism proteins mentioned above and 5-methyltetrahydropteroyltriglutamate-homocysteine ​​methyltrans...

Embodiment 3

[0174] Dot blot-like detection of a variety of 45 unique differentially expressed proteins

[0175] Target

[0176] The goals included confirming the protein leads obtained from the DIGE experiments of Example 1 in larger populations of high- and low-yielding oil palms.

[0177] method

[0178] Samples: Mesocarp tissues of 8 high-yielding palms and 8 low-yielding palms obtained at 6 time points, ie, 12 weeks, 14 weeks, 16 weeks, 18 weeks, 20 weeks and 22 weeks after pollination, were used.

[0179] Antibodies: As described in Table 3, antibodies to 27 of the 45 unique differentially expressed proteins were obtained from different suppliers.

[0180] Protein extraction (TCA extraction): Pre-cooled TCA extraction buffer (containing 10% TCA (10 g), 0.007% DTT (70 mg) and acetone (to 100 ml), stored at -20 ° C) at -20 ° C ( 0.2g+0.5ml) was added to the mesocarp sample in fine powder form. The samples were further ground with a small plastic grinder. The samples were mix...

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Abstract

Methods are provided for obtaining a high-yielding oil palm plant, comprising determining the level of a protein in mesocarp tissue of a fruit of a parental oil palm plant, determining whether there is a difference between the level of the protein in the mesocarp tissue of the fruit of the parental oil palm plant and the level of the protein in mesocarp tissue of a fruit of a reference oil palm plant, and selecting progeny of the parental oil palm plant based on the difference to obtain the high-yielding oil palm plant. Also provided are methods for predicting oil yield of a test oil palm plant and kits for obtaining a high-yielding oil palm plant.

Description

technical field [0001] The present application relates to methods of obtaining high-yielding plants, and more particularly, methods of obtaining oil palm plants high-yielding in the production of palm oil. Background technique [0002] The African oil palm Elaeis guineensis Jacq. is an important oil-food crop. Oil palms are monoecious, meaning that a single plant produces male and female flowers. Oil palms are characterized by dioecious inflorescences. The male inflorescence consists of many spikelets and can have over 100,000 flowers. Oil palms are naturally cross-pollinated by insects or wind. The female inflorescence is a spadix, which can contain thousands of flowers on spiny spikes. Each bunch carries 500 to 4000 fruits. The fruit of the oil palm is a sessile drupe. The shape is spherical to oval or elongated. The fruit consists of an exocarp, a palm oil-containing mesocarp, and an endocarp surrounding the core. [0003] Oil palm is important due to its high yie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/573C11B1/00A01H6/00A01H5/08A01H5/10
CPCG01N33/56961A01H1/04A01H5/08A01H5/10C07K14/415G01N2333/415A01H6/00
Inventor 托尼·黄英强伦纳德·丹妮拉·杰弗里·戴姆叶万晋恩波泽雅李方晋伊努尔·马斯尼·彼特·欧斯曼哈瑞科萨那·欧·库拉瓦森格玛穆赫达·纳齐尔·巴斯瑞穆海密·穆罕默德
Owner SIME DARBY PLANTATION INTPROP SDN BHD
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