Freezing and slicing method suitable for quickly observing oil palm leaf anatomical structures

A frozen section and blade technology, which is applied in the field of plant tissue frozen section preparation, can solve the problems that have not yet been seen in the research on the anatomical structure of oil palm leaves and can not be fully applied to the research on oil palm tissue slices, and achieve clear microscopic results, short time, The effect of uniform thickness

Inactive Publication Date: 2014-07-16
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

It is an important issue to explore suitable frozen section techniques for the anatomical structure and cytology of oil palm tissues. Although there have been reports on frozen sections of other plant tissues before, different types of plant tissues ha

Method used

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  • Freezing and slicing method suitable for quickly observing oil palm leaf anatomical structures
  • Freezing and slicing method suitable for quickly observing oil palm leaf anatomical structures
  • Freezing and slicing method suitable for quickly observing oil palm leaf anatomical structures

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Embodiment 1

[0020] Before slicing, the Leica cryostat (model LEICA CM1900) was pre-cooled, the temperature of the freezer was set at -20°C, and the temperature of the sample head was set at -10°C. This process takes different time to complete according to different external ambient temperatures. Generally, the set conditions can be reached after 1 to 2 hours. After the various parts of the instrument reach the set temperature, start the following operations.

[0021] 1. Choose thick white A4 printing paper, fold and cut it into a small paper frame with a length, width, and height of 5mm as the embedding box, which is used to place the sample tissue and embedding agent to be cut.

[0022] 2. Add a small amount of embedding agent to the sample tray, then place the embedding carton vertically, then put the sample tray into the pre-cooled microtome box, freeze at -20°C, OCT freezes the embedding agent Quickly solidify into a white solid substance to achieve the purpose of fixing the embedding...

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Abstract

The invention relates to a freezing and slicing method suitable for quickly observing oil palm leaf anatomical structures. The freezing and slicing method comprises the following steps: firstly setting temperatures of a freezing box and a sample head of a freezing microtome, manufacturing an embedding paper box and fixing the embedding paper box in a sample plate; selecting oil palm leaf material and trimming the oil palm leaf material into samples; adding an OCT (Optical Coherence Tomography) frozen embedding agent into the embedding paper box, then vertically putting the samples into the embedding agent, freezing under the condition of the temperature of 20 DEG C below zero till the frozen embedding agent is fully solidified, quickly trimming embedding blocks and then slicing and sealing to obtain a slice for observing oil palm leaf anatomical structures, observing the slice on a microscope to obtain images. By adopting the freezing and slicing technology, the frozen slice with the integrated oil palm leaf structures can be quickly obtained; the slice is smooth, free of wrinkles, uniform in thickness and clear in microscopy result; the slice facilitates the development of observation and research of the oil palm leaf anatomical structures and has great significance to the work of cultivating varieties suitable for cultivation in China or screening high-quality germplasm resource types.

Description

technical field [0001] The invention belongs to the technical field of microscopic observation samples, and relates to a method for preparing frozen section of plant tissue, in particular to a frozen section method suitable for quickly observing the anatomical structure of oil palm leaves based on frozen section technology, including a method suitable for freezing oil palm leaf tissue. Parameter setting of cryostat for sectioning and making of embedding carton, sample fixing, slicing, sealing and observation, etc. Background technique [0002] Oil palm (Elaeis guineensis Jacq.) is the oil-producing plant with the highest production efficiency in the world, and has broad prospects for development and utilization. However, the oil palm industry in my country started relatively late, and its varieties are mainly imported from abroad. There is no oil palm with independent intellectual property rights. New varieties, while oil palm varieties imported from abroad need long-term int...

Claims

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Application Information

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IPC IPC(8): G01N1/06G01N1/42G01N1/36
Inventor 张大鹏曹红星
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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