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Oil palm tissue culture method

A technology of tissue culture and oil palm, which is applied in the field of medium for plant tissue culture, to achieve the effect of shortening the reproduction cycle

Inactive Publication Date: 2013-01-09
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on oil palm tissue culture medium and culture methods in China, especially the method of using non-oil palm seed embryos as explant materials for propagation

Method used

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  • Oil palm tissue culture method
  • Oil palm tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Selection of explants

[0027] Select 5 oil palm high-yield and high-quality mother plants with an age of 8 years, cut the oil palm stems at 15cm to 95cm above the stem growth point, remove the surface impurities from the obtained oil palm stems, and spray 75 % alcohol is sterilized, dried, and then the dried oil palm stems are transferred to the ultra-clean workbench, and the old leaves of the outer layer are peeled off to obtain aseptic young oil palm leaves.

[0028] 2. Induction culture of callus

[0029] Trim oil palm tender leaves into pieces with a size of 1cm×2cm, inoculate them into callus induction medium, and carry out dark culture at a temperature of 25-28°C and a humidity of 50%-65%, and cultivate for 100 The callus was obtained after 1 day; the obtained callus was transferred to the embryogenic callus culture medium, and cultured in dark at a temperature of 25-28°C and a humidity of 50%-65%, and the embryos were obtained after 90 days of culture. sexu...

Embodiment 2

[0037] Select 5 oil palm high-yield and high-quality mother plants with a tree age of 9 years, cut the oil palm stems at 15cm to 95cm above the stem growth point, remove the surface impurities from the obtained oil palm stems, and spray 75 % alcohol is sterilized, dried, and then the dried oil palm stem is transferred to an ultra-clean workbench, and the old leaves of the outer layer are peeled off to obtain aseptic young oil palm leaves.

[0038] 2. Induction culture of callus

[0039] Trim oil palm tender leaves into pieces with a size of 1cm×2cm, inoculate them into callus induction medium, and carry out dark culture at a temperature of 25-28°C and a humidity of 50%-65%, and cultivate for 100 The callus tissue was obtained after 1 day; the obtained callus tissue was transferred into the embryogenic callus medium, and cultured in the dark at a temperature of 25-28°C and a humidity of 50%-65%, and the embryonic tissue was obtained after 90 days of culture. sexual callus.

...

Embodiment 3

[0047] Select 5 oil palm high-yielding and high-quality mother plants with a tree age of 10 years, cut off the oil palm stems at 15cm to 95cm above the stem growth point, remove the surface impurities from the obtained oil palm stems, and spray 75% on the surface of the oil palm stems % alcohol is sterilized, dried, and then the dried oil palm stems are transferred to the ultra-clean workbench, and the old leaves of the outer layer are peeled off to obtain aseptic young oil palm leaves.

[0048] 2. Induction culture of callus

[0049] Trim oil palm tender leaves into pieces with a size of 1cm×2cm, inoculate them into callus induction medium, and carry out dark culture at a temperature of 25-28°C and a humidity of 50%-65%, and cultivate for 100 The callus tissue was obtained after 1 day; the obtained callus tissue was transferred into the embryogenic callus medium, and cultured in the dark at a temperature of 25-28°C and a humidity of 50%-65%, and the embryonic tissue was obtai...

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Abstract

The invention belongs to a culture medium for plant tissue culture, particularly relates to an oil palm tissue culture method. An oil palm stem section on an oil palm stock plant stem growing point is cut, and then oil palm tender leaves are pruned into plates and inoculated onto a callus induction culture medium, an embryonic callus culture medium and an embryoid culture medium to be cultured to obtain oil palm somatic embryos; and the somatic embryos are inoculated into a bud induction culture medium, a rooting culture medium and a liquid rooting culture medium to be cultured to obtain oil palm test-tube plantlets. Oil palm tender leaves are used as explant material, a tissue culture technique is utilized to rapidly propagate oil palm without the limitation of seasons and weather, oil palm descendants can be effectively propagated largely, the propagation cycle of ordinary breeding is greatly shortened, the genetic characters of descendants are highly consistent to those of the parents, and an effective path is provided for the wide popularization and application of oil palm fine breeds.

Description

technical field [0001] The invention belongs to a culture medium for plant tissue culture, and in particular relates to a method for culturing oil palm tissue. The culture medium is used to cultivate and rapidly propagate oil palm tissue, providing technical support for the large-scale popularization and application of improved oil palm varieties. Background technique [0002] The origin of oil palm is in the humid forest edge area of ​​Africa at 10° south latitude to 15° north latitude and below 150 meters above sea level. The main production areas are distributed in Malaysia, Indonesia, west and central Africa in Asia, northern South America and Central America. my country has introduced oil palms for more than 80 years, mainly distributed in Hainan, Yunnan, Guangdong and other places. Oil palm is a tropical woody oil crop, and its yield per unit area is several to dozens of times that of other oil crops. It is known as the "king of vegetable oil in the world". At present...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邹积鑫林位夫潘登浪尤丽莉张朝阳曾宪海张希财周立军姚行成曹建华
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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