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Preparation method of high-content salvianolic acid B

A technology of salvianolic acid and total salvianolic acid of salvia miltiorrhiza is applied in the field of preparation of high-content salvianolic acid B, can solve problems such as being difficult to effectively satisfy industrialization development and application, and achieve the effects of effective production cost and reduction of production cost

Active Publication Date: 2014-11-05
王萍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the above-mentioned technical methods are not easy to effectively meet the industrialization development and application of salvianolic acid B as a new preparation (90%)

Method used

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  • Preparation method of high-content salvianolic acid B
  • Preparation method of high-content salvianolic acid B
  • Preparation method of high-content salvianolic acid B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Extract 1 kg of crushed Danshen medicinal material with 7 L, 6 L, and 4 L of 70% ethanol aqueous solution under reflux for 3 times, each time for 2 hours, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and filter. The obtained 1L filtrate was extracted 6 times with 2L ethyl acetate, then the pH of the remaining aqueous phase was adjusted to 2 with 0.1mol / L hydrochloric acid, and 1L ethyl acetate was added to extract 3 times, the organic phases were combined and filtered through 20g of silica gel to obtain the filtrate After concentrating under reduced pressure to dryness, the total phenolic acids of salvia miltiorrhiza were obtained; after dissolving the total phenolic acids of salvia miltiorrhiza in water, adjust the concentrated solution pH to 6 with 1mol / L sodium bicarbonate solution, and chromatographically through 2Kg macroporous resin HP-20 (batch number : 081104, Japan Mitsubishi Chemical Company), 5% ethanol water eluent...

Embodiment 2

[0087] Extract 1 kg of the pulverized Danshen medicinal material with 7 L, 5 L, and 3 L of 80% ethanol aqueous solution successively under reflux conditions for 3 times, each time for 2 hours, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and filter. Extract 1 L of the filtrate with 2 L of propyl acetate for 5 times, then adjust the pH of the aqueous phase to 2 with 0.1 mol / L sulfuric acid, add 1 L of ethyl acetate for extraction three times, combine the organic phases and filter through 20 g of silica gel, and concentrate the filtrate to dryness under reduced pressure , to obtain the total phenolic acid of salvia miltiorrhiza; after dissolving the total phenolic acid of salvia miltiorrhiza in water, adjust the pH of the concentrated solution to 6 with 1mol / L potassium carbonate solution, and chromatographically , with 0, 5% ethanol water eluent respectively eluting 6 resin column volumes (3L), 10% ethanol eluting 2 resin column volum...

Embodiment 3

[0089] 1 kg of crushed Danshen medicinal material was extracted with 8L, 6L and 3L of 60% ethanol aqueous solution successively under reflux conditions for 3 times, each time for 2 hours, the extracts were combined, concentrated under reduced pressure until there was no alcohol smell, and concentrated liquid, filtered. Concentrate the concentration of salvianolic acid B in the filtrate to 100 mg / mL, then alcohol precipitation with 95% ethanol solution twice; filter, concentrate the filtrate, add water to dilute to the concentration of salvianolic acid B to 40 mg / mL, add 2L ethyl acetate for extraction 6 times, the remaining aqueous phase containing salvianolic acid B was concentrated under reduced pressure until there was no ethyl acetate smell; then, it was chromatographed on 3kg of macroporous resin HPD-100 (batch number: 110425, Cangzhou Baoen resin), respectively with 0, 10% , 15% ethanol water eluent respectively elute 6 resin column volumes (3L), then use 50% ethanol to ...

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Abstract

The invention discloses a preparation method of a high-content salvianolic acid B. The method comprises the following two steps: separating total salvianolic acids and preparing the high-content salvianolic acid B. According to the method, water phase is selectively removed via a method of organic solvent extraction without affecting on water-soluble components, and the rest of water phase is acidized and then extracted, so that the water-soluble components with high polarity such as inorganic salt, tannin, sugar and protein and can be selectively removed; finally, the high-content total salvianolic acid is obtained. Based on the obtained total salvianolic acid, salvianolic acid B product having content being more than 95% is finally obtained with the final yield about 3% by carrying out the steps of regulating pH of the total salvianolic acid solution within 6.0 to 8.0, separating the total salvianolic acid solution via macroporous resin and the like. The method is simple to develop, effective and low in cost, and has an industrial value.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method of high-content salvianolic acid B. technical background [0002] Salvia miltiorrhiza, a plant of the genus Salvia in the Labiatae family, its roots and stems are used as medicine, bitter in taste, slightly cold in nature; Guixin and liver meridian; it has the functions of dispelling blood stasis and relieving pain, promoting blood circulation and dredging menstrual flow, clearing heart and eliminating troubles and dilating blood vessels, etc. It is a traditional Chinese medicine widely used clinically. Modern pharmacological studies have found that Danshen has significant anti-inflammatory, antibacterial, anti-oxidation, anti-liver damage, anti-platelet aggregation, anti-atherosclerosis and anti-tumor effects. [0003] The study of the material basis shows that the main chemical components that produce the above pharmacological activit...

Claims

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Application Information

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IPC IPC(8): C07D307/86A61K31/343A61P9/00A23L1/29A23L33/00
CPCC07D307/86
Inventor 王平
Owner 王萍
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