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Total tanshinone and total phenolic acid extract in red-rooted salvia root and its production

A kind of technology of total tanshinone and total phenolic acid, applied in the field of medicine

Active Publication Date: 2007-11-21
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, so far, there are no patents related to the total tanshinone and total phenolic acid extracts in Salvia miltiorrhiza and their preparation methods and uses.

Method used

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  • Total tanshinone and total phenolic acid extract in red-rooted salvia root and its production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Preparation process of total tanshinone and total phenolic acid extract of Salvia miltiorrhiza

[0055] Take 1kg of Danshen decoction pieces, extract 3 times with 10L of 90% ethanol under reflux, extract for 2 hours each time, recover the solvent under reduced pressure, and obtain the crude extract of total tanshinone; solvent to obtain the crude extract of total phenolic acids. The total tanshinone crude extract was dispersed and dissolved by adding 50% ethanol to make the solution concentration 0.70 mg / mL (calculated by the total tanshinone amount), and the ultrasonic wave was used to dissolve and disperse evenly. The suspension was taken and passed through 1.8L AB-8 macroporous adsorption resin. The adsorption flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:6, the sample loading is 2.1mg / mL (in terms of total tanshinone), and after washing 3 times the volume of the resin, 70% ethanol is used to elute 7 times the volume of the ...

Embodiment 2

[0056] Example 2: Preparation process of total tanshinone and total phenolic acid extract of Salvia miltiorrhiza

[0057] Take 2 kg of Danshen decoction pieces, extract 3 times with 15 L of 95% ethanol under reflux, extract 1.5 hours each time, recover the solvent under reduced pressure, and obtain the crude extract of total tanshinone; extract the dregs with 10 L of 5% ethanol under reflux three times, extract 1 hour each time, The solvent was recovered under reduced pressure to obtain a crude extract of total phenolic acids. The total tanshinone crude extract was dispersed and dissolved by adding 45% ethanol to make the solution concentration 0.60 mg / mL (calculated by the amount of total tanshinone). Ultrasound was used to dissolve and disperse evenly. The suspension was taken and passed through 3L AB-8 macroporous adsorption resin for adsorption The flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:6, the sample loading is 1.8mg / mL (based on the ...

Embodiment 3

[0058] Example 3: Preparation process of total tanshinone and total phenolic acid extract of Salvia miltiorrhiza

[0059] Take 5kg of Danshen decoction pieces, extract 3 times with 10L of 90% ethanol under reflux, extract for 1 hour each time, recover the solvent under reduced pressure, and obtain the crude extract of total tanshinone; solvent to obtain the crude extract of total phenolic acids. The total tanshinone crude extract is dispersed and dissolved by adding 50% ethanol to make the solution concentration 0.64mg / mL (calculated by the amount of total tanshinone), ultrasonically dissolves and disperses evenly, takes the suspension, passes through 2L AB-8 macroporous adsorption resin, and absorbs The flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:6, the sample loading is 2.0mg / mL (based on the amount of total tanshinone), wash with water for 3 times the volume of the resin, and then elute with 70% ethanol for 8 times the volume of the resin f...

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Abstract

The invention is concerned with a kind of extract of total ketone of salviae miltiorrhizae and total phenolic acid and its produce method form radix Salviae Miltiorrhizae. The extract of total ketone of salviae miltiorrhizae has cryptotanshinone, tanshinone I, tanshinone IIA, methyl Tanshinon, dihydrotanshinon I and ramification. The extract of total phenolic acid has salvianolic acid A, salvianolic acid B, protocatechuic aldehyde and ramification. The extract can be got form one or arbitrary compound of extraction with solvent method, macroporous resin method, column chromatography and liquid-liquid counter-current chromatography. The summation of the content to each total ketone of salviae miltiorrhizae is 20 to 100 percnte (w / w) of the extract of total ketone of salviae miltiorrhizae, the contene of cryptotanshinone, tanshinone I and tanshinone IIA is 5 to 100 percent (w / w) of whole content of total ketone of salviae miltiorrhizae. The summation of the content to each total phenolic acid is 5 to 100 percent (w / w) of the extract of the radix salviae miltiorrhizae total phenolic acid. The content of salvianolic acid B is the 5 to 100 percent (w / w) of the whole salvianolic acid.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an extract of total tanshinone and total phenolic acid of the traditional Chinese medicine Salvia miltiorrhiza, a preparation method, a quality control method and its application in the fields of medicine and health food. Background technique [0002] Salvia miltiorrhiza is a commonly used traditional Chinese medicine, derived from the dried roots and rhizomes of Salvia miltiorrhiza Bunge, a plant of the Labiatae family. Traditional Chinese medicine theory believes that Danshen has the effects of dispelling blood stasis and relieving pain, promoting blood circulation and regulating menstruation, nourishing the heart and eliminating troubles. Modern pharmacological studies have shown that Salvia miltiorrhiza has various effects such as cerebral ischemia protection, improving coronary blood flow and microcirculation, promoting tissue repair, anti-platelet aggregation, ...

Claims

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Application Information

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IPC IPC(8): A61K36/537A61K9/00A23L2/52A61P9/10A61P27/12A61P31/04A61P35/00C07D307/94C07D307/42C07C67/48A61K125/00
Inventor 石任兵刘斌宋文婷
Owner 石任兵
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