Method for separating and purifying salvianolic acid from red sage root liquid extract by one step

Abstract

The invention relates to a method for furthering separating and purifying salvianolic acid from Danshen extract fluid, which comprises that prepares, breaks and extracts Danshen via water solution, acidifies extract to adjust pH and adds salt to process post-treatment, processes dynamic continuous adsorption and elution on the treated extract at chromatography column stuffed with resin adsorbent, elutes via water, collects and concentrates eluent, elutes via gradient ethanol solution, segmented collects and concentrates eluent, and dries the concentrates solution to obtain product. The invention can simply, effectively and quickly separate and purify various salvianolic acids, wherein test on different salvianolic acid products shows that the highest yields of tanshinol, alkannic acid, rosmarinic acid, salvianolic acid A and salvianolic acid B are 35. 55%, 70.65%, 99.27%, 82.78%, and 89.34%, and relative highest purities are 95.32%, 65.05%, 29.40%, 33.93%, and 82.35%, which are near or higher than the result of purification on the goal of single component.

Description

technical field [0001] The present invention relates to extraction technology of functional components of natural plants, especially a method for one-step separation and purification of salvianolic acid from water extract of salvia miltiorrhiza Methods. Background technique [0002] Salvia (Salvia miltiorrhiza Bunge) is a perennial herb belonging to the genus Salvia of the Labiatae family. Its dry root is a traditional Chinese medicine that has a long history of promoting blood circulation and removing stasis. Blood, promoting blood circulation and removing blood stasis, nourishing blood and nourishing Qi, dispelling numbness and strengthening feet, regulating menstruation and stopping collapse, detoxification and expulsion of pus". [0003] The water-soluble components of Danshen have a wide range of pharmacological effects, such as protecting myocardial ischemia and hypoxia, improving microcirculation, central sedation, inhibiting platelet aggregation and thrombus formati...

AI Technical Summary

Problems solved by technology

[0004] At present, the water-soluble components of Salvia Miltiorrhiza are mostly obtained by methods such as water extraction, alcohol precipitation, and ethyl acetate extraction. Literature (Chinese Patent Medicine, 2007, 29(1): 143-144; Chinese Herbal Medicine, 2007, 38(6): 843-846 ; Chinese Herbal Medicine, 2007, 38 (4): 542-545; Shi Zhen Guoyi Guoyao, 2007, 18 (5): 1186-1187) are all elaborated in detail, but the resulting product may be a mixture of polyphenolic acids, and the structure-activity relationship is not Clear; or after multi-step purification to obtain a single medicinal substance, such as CN1342638A adopts water extraction-double column adsorption elution-solvent extraction and other steps to purify Danshensu, CN1303052C adopts water or alcohol extraction-precursor conversion-ultrafiltration to remove Miscellaneous-solvent extraction-normal phase chromatography and other multi-step purification of Danshensu, CN1751706A adopts alkali extraction-acidification-alcohol precipitation-double column (resin+polyamide) adsorption elution-crystallization and other multi-step purification of Danshensu, CN101012163A adopts resin adsorption washing De-recrystallization or secondary chromatographic purification of Danshensu, CN1425659 uses several steps such as water extraction-acidification-column chromatography-drying to separate and purify salvianolic acid B. In the above examples, a single component is used as the extraction and purification target, and other Functional ingredients not involved

CN1305866C adopts twice boiling water extraction-high-speed centrifugation-membrane filtration to remove impurities or solvent extraction-reversed phase chromatographic separation to obtain three components of salvianolic acid A, B and protocatechuic aldehyde, but parameters such as purity and yield have not been determined. Reported, and batch processing, each loading 5ml

Therefore, there is no method that is easy to operate, has high extraction efficiency, product purity and throughput, and can simultaneously separate and purify multiple salvianolic acids.

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