Method for preparing salvianolic acid B magnesate

A technology of salvianolic acid and magnesium salt, which is applied in the field of extraction and separation of botanical medicinal materials, can solve the problems of high price, high cost and small output, and achieves the effects of simple process, low price and simple equipment.

Inactive Publication Date: 2004-04-14
上海天甲生物医药有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is: in the prior art, the extraction and separation method of salvianolic acid B magnesium salt is to pulverize the medicinal material into powder, soak in 70% acetone, concentrate under reduced pressure, and then further absorb it through macropores. Resin, molecular sieve and other chromatographic methods are obtained, so this method needs to use a variety of expensive chromatographic packing materials, and there are small yields, high costs, and the purity of the obtained salvianolic acid B magnesium salt is not high, which cannot be industrialized. Production, etc. shortcoming

Method used

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  • Method for preparing salvianolic acid B magnesate

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Effect test

Embodiment 1

[0019] 5 kg of Salvia miltiorrhiza medicinal materials, crushed through a 5-mesh sieve, soaked with a total amount of 20L of 60% acetone at 20°C for 6 hours, then released the soaking liquid. The solvent was evaporated under reduced pressure until there was no acetone smell, the precipitate was removed by centrifugation at 2000 rpm, and the D101 or D520 type macroporous adsorption resin was used for chromatography, eluted with water to remove impurities, then eluted with 20% ethanol, recovered ethanol, and freeze-dried. After the obtained product is dissolved in water, it is chromatographed with polyamide to remove impurities with water, and then eluted with 5% ethanol to obtain the salvianolic acid B magnesium salt component, which is concentrated under reduced pressure and freeze-dried. The content of the magnesium salt of salvianolic acid B determined by HPLC was 93.2%.

Embodiment 2

[0021] 5 kg of Salvia miltiorrhiza medicinal materials were crushed to 20 meshes, and the soaking liquid was released after being soaked in a total of 20 L of 80% ethanol at 20°C for 12 hours. The solvent was evaporated under reduced pressure until there was no alcohol smell, and the precipitate was removed by centrifugation at 4000 rpm. The AB-8 type macroporous adsorption resin was used for chromatography, eluted with water to remove impurities, then eluted with 30% ethanol, recovered ethanol, and freeze-dried. After the obtained product is dissolved in water, it is chromatographed with polyamide to remove impurities with water, and then eluted with 10% ethanol to obtain the salvianolic acid B magnesium salt component, which is concentrated under reduced pressure and freeze-dried. The content of magnesium salt of salvianolic acid B determined by high performance liquid chromatography was 90.8%.

Embodiment 3

[0023] Salvia miltiorrhiza medicinal material 5kg, crushed through a 10-mesh sieve, soaked with a total of 20L of 80% acetone at 40°C for 8 hours, then released the soaking liquid. The solvent was evaporated under reduced pressure until there was no acetone smell, and the precipitate was removed by centrifugation at 2000 rpm. The NKA-II type macroporous adsorption resin was used for chromatography, eluted with water to remove impurities, and then eluted with 20% and 40% ethanol successively to recover 40% ethanol Elution site. Then it was chromatographed with NKA-II type macroporous adsorption resin, eluted with water to remove impurities, collected the 20% ethanol eluted fraction, and freeze-dried. After the obtained product was dissolved in water, it was chromatographed with polyamide to remove impurities with water, and then eluted with 20% ethanol to obtain the component containing the magnesium salt of salvianolic acid B, concentrated under reduced pressure, freeze-dried, and...

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Abstract

The invention provides a manufacturing method of red phenolic acid B magnesium salts. Crushes the radix salviae miltiorrhizae, immerses and extracts with alcohol containing water or acetone, condenses, eliminates the sediment. Then uses macropore to absorb resin chromatography, washes them with water, then wash with alcohol. The coarse product is obtained after condensed, then carries on chromatography with polyamide, and washes with water or alcohol. There gets the 90% purity product through condensation, cooling, drying.

Description

Technical field: [0001] The invention relates to the field of extraction and separation of plant medicinal materials, in particular to a method for extracting and separating the salvianolic acid B magnesium salt from the traditional Chinese medicinal material Salvia miltiorrhiza. Background technique: [0002] Salvia miltiorrhiza Bge, a plant of the labiatae family, is a perennial herb that has the effects of removing blood stasis and relieving pain, promoting blood circulation, clearing the heart and removing troubles. In modern clinics, it is widely used to prevent and treat cardiovascular disease, kidney disease, liver disease, etc. [0003] Magnesium Lithospermate B (Magnesium Lithospermate B) is a water-soluble phenolic acid substance isolated from Salvia miltiorrhiza. Its molecular formula is C 36 H 28 O 16 Mg, the structural formula is: [0004] [0005] Salvianolic acid B magnesium salt has the functions of protecting liver and cardiovascular system. [0006] The extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/84
Inventor 顾心彬朱洪莉
Owner 上海天甲生物医药有限公司
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