Medicinal composition containing general ginsenoside and total salvianolic acid and preparation method thereof

A technology of total ginsenosides and total phenolic acids of salvia miltiorrhiza is applied in the directions of drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., which can solve the problems of no pharmacological action and research on its mechanism, and achieve the effect of expanding the scope of medicinal use.

Active Publication Date: 2011-06-22
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no systematic studies have been conducted on the pharmacological effects and mechanisms of medicinal compositions made from Danshen and Panax ginseng on blood vessels

Method used

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  • Medicinal composition containing general ginsenoside and total salvianolic acid and preparation method thereof
  • Medicinal composition containing general ginsenoside and total salvianolic acid and preparation method thereof
  • Medicinal composition containing general ginsenoside and total salvianolic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of a pharmaceutical composition comprising total ginsenosides and salvianolic acids

[0029] Add 60% (volume / volume) ethanol aqueous solution to ginseng decoction pieces and heat and reflux for extraction 4 times. The weight of the alcohol-added solution is 6, 4, 4 and 4 times the weight of ginseng decoction pieces in turn, and the extraction time is 0.5 hours each time. Combine the extracts, pass through HPD-100 type macroporous adsorption resin after reclaiming ethanol, first use 6 times of column volume after the water elution, then successively use 20% (volume / volume) of 8 times of column volume and 6 times of column volume The 60% (volume / volume) ethanol aqueous solution was eluted respectively, and the eluate of the 60% (volume / volume) ethanol aqueous solution was collected, concentrated, and spray-dried to obtain the total ginseng saponins extract.

[0030] Add deionized water to Danshen decoction pieces, heat and extract 3 times at 80°C, t...

Embodiment 2

[0032] Embodiment 2: the content determination of total ginseng saponins

[0033] The content assay method of 8 kinds of main active components in the total ginseng saponins extract prepared in embodiment 1 is as follows:

[0034] Preparation of mixed reference substance solution: Accurately weigh ginsenoside Rg respectively 1 , Ginsenoside R e , Ginsenoside Rg 2 , Ginsenoside Rb 1 , Ginsenoside R c , Ginsenoside Rb 2 , Ginsenoside Rb 3 and ginsenoside R d Appropriate amount of reference substance, add methanol to make concentration Rg 1 (0.488mg / ml), R e (0.260mg / ml), Rg 2(0.163mg / ml), Rb 1 (0.656mg / ml), Rb 2 (0.310mg / ml), R c (0.454mg / ml), Rb 3 (0.101mg / ml) and Rd (0.164mg / ml) mixed reference solution. Ginsenoside Rh made by the same method 1 (0.406mg / ml) reference substance solution.

[0035] The preparation of need testing solution: get total ginsenosides 12.5mg, accurately add 50% (volume / volume) methanol aqueous solution 20ml, close up, weigh, sonicate 30...

Embodiment 3

[0040] Embodiment 3: the assay of total phenolic acid extract of Salvia miltiorrhiza

[0041] The content assay method of 5 kinds of main active components in the total phenolic acid extract of salvia miltiorrhiza prepared in embodiment 1 is as follows:

[0042] Preparation of mixed reference substance solution: Accurately weigh the appropriate amount of Danshensu sodium, protocatechualdehyde, rosmarinic acid, shikonian acid and salvianolic acid B reference substance respectively, and add methanol to make concentrations of 0.079, 0.0228, 0.0294, 0.0378 And 1.12mg / ml mixed reference substance solution.

[0043] The preparation of need testing solution: get the total phenolic acid extract of salvia miltiorrhizae 12.5mg, accurately add 50% (volume / volume) methanol aqueous solution 20ml, close up, weigh, sonicate 30min, let cool, weigh again, use Make up the lost weight with methanol, filter through 0.22μm membrane, and take the subsequent filtrate as the test solution.

[0044]...

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Abstract

The invention provides a medicinal composition containing general ginsenoside and total salvianolic acid and a preparation method thereof. The preparation method comprises the following steps of: performing heating reflux on ginseng in 60 percent (volume/volume) of aqueous solution of ethanol, after recovering the ethanol, allowing extract to pass through macroporous adsorbent resin, eluting by using water, 20 percent (volume/volume) aqueous solution of ethanol and 60 percent (volume/volume) aqueous solution of ethanol respectively, collecting a part which is eluted by 60 percent (volume/volume) aqueous solution of ethanol, and removing solvent to obtain general ginsenoside extract; leaching root of red-rooted salvia in water by heating, adding the ethanol into the extract until the volume of the ethanol is 80 percent of that of the extract, standing, filtering, after recovering the ethanol, allowing filter liquor to pass through the macroporous adsorbent resin, eluting by using the water and 40 percent (volume/volume) of aqueous solution of ethanol, collecting a part which is eluted by 40 percent (volume/volume) of aqueous solution of ethanol, and removing the solvent to obtain total salvianolic acid extract; and mixing the general ginsenoside extract and the total salvianolic acid extract according to a weight ratio of (0.1-10):(0.1-10).

Description

technical field [0001] The invention relates to a pharmaceutical composition containing traditional Chinese medicine extracts and a preparation method thereof, in particular to a pharmaceutical composition containing total ginsenosides and salvianolic acids and a preparation method thereof. Background technique [0002] Hypertension is a common cardiovascular disease in my country, with a high prevalence rate. The mortality and disability rate caused by vascular complications such as heart, brain, kidney, and limbs ranks first among all kinds of diseases, and it is extremely harmful to human health. . At present, the treatment of hypertension and its related vascular complications mainly relies on western medicine, and the treatment of hypertension based on traditional Chinese medicine has not yet made a breakthrough. Therefore, how to develop the traditional medicine of the motherland and use the unique resources of our country to develop the application of traditional Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61P9/10A61P9/12
Inventor 屠鹏飞张可马旭马治中
Owner PEKING UNIV
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