A fungicidal composition for preventing and treating guava anthracnose

Abstract

The present application belongs to the technical field of guava disease prevention and treatment, and particularly relates to a kind of fungicide composition for preventing and treating guava anthracnose.A kind of fungicide composition for preventing and treating guava anthracnose, its effective component is compounded by rock white menu lactone and berberine or flutriafol, wherein, the mass ratio of rock white menu lactone and berberine is 1-7:15-1;The mass ratio of rock white menu lactone and flutriafol is 1-20:20-1.Rock white menu lactone and berberine or flutriafol are compounded in the present application, and the synergistic coefficient SR for inhibiting the mycelium growth of guava anthracnose pathogenic bacteria is greater than 1.5, which shows synergistic effect.Compared with single component, the prevention and treatment effect on guava anthracnose can be improved.In addition, the fungicide composition of the present application is compounded by rock white menu lactone and berberine or flutriafol, compared with single component, the generation and development of pathogenic bacteria resistance can be delayed, and the service life of the pesticide can be prolonged.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of guava disease prevention and treatment, and particularly relates to a fungicidal composition for preventing and treating guava anthracnose. BACKGROUND

[0002] Guava is also known as Passiflora edulis, egg fruit, which is rich in nutrients such as vitamins, amino acids, mineral elements and dietary fiber, as well as bioactive ingredients such as flavonoids, carotenoids and polysaccharides, and is deeply loved by consumers. The main diseases of guava in China include viral diseases, anthracnose, stem base rot and fruit rot, etc. Among them, the main pathogens of guava anthracnose are Colletotrichum gloeosporioides, C. acutatum, C. boninense and C. truncatum, etc., which mainly harm the fruit. At the early stage of the disease, small yellow spots appear, which gradually expand into yellow spots, and when the disease is severe, the spots are connected, and small black spots appear on the spots at the later stage, which seriously affects the yield and quality of guava.

[0003] Chemical fungicides are the most economical and effective means for disease control, which has extremely important significance in improving the yield and quality of agricultural products. However, long-term continuous use of single variety or single action mode of fungicides at high doses can easily cause the generation and evolution of pathogen resistance. Reasonable compounding of fungicides with fungicidal activity can improve the control effect, delay the generation and evolution of pathogen resistance, etc., which is one of the most effective methods to solve the above problems.

[0004] Coumarin is a general term for a class of natural compounds, which has aromatic odor. Coumarin compounds are distributed in plants of 73 families and more than 330 genera, especially in angiosperms such as Umbelliferae, Rutaceae and Leguminosae, which have been widely used as important raw materials in the fields of spices, cosmetics, medicine, electroplating and plastic industry, etc. There are also relevant reports in existing Chinese patents that coumarin can be used as an agricultural fungicide. For example, Li Minmin et al. isolated and identified active substances in Angelica pubescens for inhibiting Colletotrichum gloeosporioides, and obtained four coumarin compounds with inhibitory effect on crop pathogens: isoimperatorin, osthole, pimpinellein and isopimpinellein. Wang Xuelian et al. determined the inhibitory effect of 18 coumarin compounds on Colletotrichum gloeosporioides by growth rate method, and found that the 18 coumarin compounds had different degrees of inhibitory effect on Colletotrichum gloeosporioides within the test range, among which, the inhibitory activity of umbelliferone (CAS: 477-90-7) on Colletotrichum gloeosporioides was the strongest, and the EC50 was 7.3766 μg / mL.

[0005] At present, there is no relevant report on the use of umbelliferone, berberine or fluazinam in combination for preventing and treating guava anthracnose. SUMMARY

[0006] The present application aims to provide a kind of preventing and treating guava anthracnose fungicidal composition, to solve the problem of using single fungicidal active ingredient.

[0007] To achieve the above object, the present application provides the following technical solutions:

[0008] A kind of preventing and treating guava anthracnose fungicidal composition, its effective component is compounded by rock white inside lactone and berberine or fluazinam, wherein,

[0009] The mass ratio of rock white inside lactone and berberine is 1-7:15-1;

[0010] The mass ratio of rock white inside lactone and fluazinam is 1-20:20-1.

[0011] More effective, the mass ratio of rock white inside lactone and berberine is 1:7.

[0012] More effective, the mass ratio of rock white inside lactone and fluazinam is 10:1.

[0013] More specifically, the pathogenic bacteria of guava anthracnose is Colletotrichum gloeosporioides, Colletotrichum truncatum, Colletotrichum boninense or Colletotrichum brasiliense.

[0014] Compared with the prior art, the present application has the following beneficial effects:

[0015] (1) Rock white inside lactone and berberine or fluazinam are compounded in the present application, and the synergistic coefficient SR of inhibiting the mycelial growth of guava anthracnose pathogenic bacteria is greater than 1.5, showing synergistic effect. Compared with single component, the prevention and treatment effect on guava anthracnose can be improved.

[0016] (2) The fungicidal composition of the present application is compounded by rock white inside lactone and berberine or fluazinam, compared with single component, the production and evolution of pathogenic bacteria resistance can be delayed, and the service life of the pesticide can be prolonged. DETAILED DESCRIPTION

[0017] The technical solutions of the present application will be described below in a clear and complete manner. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0018] Example: virulence determination of single dose and mixed preparation on guava anthracnose pathogen

[0019] 1. Test strain

[0020] Colletotrichum brasiliense isolated from guava anthracnose typical symptom fruit

[0021] 2. Test agents

[0022] 98% of the original drug, 98% of the original drug, 98% of the original drug

[0023] After the test agent is completely dissolved, a single dose stock solution with an active ingredient concentration of 5000 mg / L is prepared by diluting the Tween-80 aqueous solution with a volume fraction of 0.1%. Multiple groups of mass ratios are set (see Tables 1 and 2), and each single dose stock solution and the mixed agent are set with 5 mass concentration gradients by equal ratio method.

[0024] 3. Test method

[0025] The mycelial growth rate method was used. Based on the preliminary test, the pre-melted PDA medium was quantitatively added to the sterile conical flask. When the PDA medium cooled to about 50℃, the liquid medicine was added to the sterile conical flask, the volume ratio of the liquid medicine and PDA medium was 1:9, and then mixed uniformly. After that, it was poured into a glass culture dish with a diameter of 90mm, 10mL of the medicine-containing medium was poured into each culture dish, and then cooled to form a medicine-containing plate. At the same time, the control plate with a volume ratio of 0.1% Tween-80 aqueous solution and PDA medium was set to 1:9. 0.1% Tween-80 aqueous solution and each mass concentration of liquid medicine were set with 5 plates.

[0026] The mycelium of the test strain was picked on PDA medium and cultured for 3 days. Then a 6mm diameter agar cake was cut from the edge of the colony using a puncher. Then the agar cake was inoculated in the center of the medicine-containing plate and the control plate, with one agar cake per dish. The mycelium was tightly attached to the medium, and then the dish was covered and placed in a constant temperature light incubator at 25℃. After 4 days, it was taken out and the colony diameters of each treatment were measured using the cross method. The average colony diameter and mycelial growth inhibition rate were calculated.

[0027]

[0028] 4. Data analysis

[0029] The logarithmic values of the drug concentration and the probability values of the control effect were subjected to regression analysis to calculate the EC 50 of each agent. The synergistic coefficient SR of the mixed agent was calculated according to Wadley method.

[0030] The synergistic effect of mixed agents was evaluated according to the synergistic coefficient SR. SR≥1.5 indicates synergistic effect; SR≤0.5 indicates antagonistic effect; 0.5

[0031] Table 1 Toxicity determination results of rock lily lactone and berberine ratio on guava anthracnose pathogen Colletotrichum brasiliense

[0032]

[0033] From Table 1, it can be seen that when rock lily lactone and berberine are compounded in the mass ratio range of 1-15:15-1, the synergistic coefficient SR is between 0.5-1.5 when the mass ratio of the two is 15:1, showing an additive effect type, and the synergistic coefficient SR of the rest of the mass ratio is greater than 1.5, showing a synergistic effect type, especially when the value ratio is 1:7, the synergistic coefficient SR is the largest. It shows that rock lily lactone and berberine can improve the control effect on guava anthracnose pathogen Colletotrichum brasiliense.

[0034] Table 2 Toxicity determination results of rock lily lactone and pydiflumetofen ratio on guava anthracnose pathogen Colletotrichum brasiliense

[0035]

[0036] From Table 2, it can be seen that when rock lily lactone and berberine are compounded in the mass ratio range of 1-20:20-1, the synergistic coefficient SR of each mass ratio is greater than 1.5, showing a synergistic effect type, especially when the mass ratio is 10:1, the synergistic coefficient SR is the largest. It shows that rock lily lactone and pydiflumetofen can improve the control effect on guava anthracnose pathogen Colletotrichum brasiliense.

[0037] In summary, when rock lily lactone and berberine or pydiflumetofen are compounded, the synergistic coefficient SR for inhibiting guava anthracnose pathogen mycelial growth is greater than 1.5, showing a synergistic effect. Compared with a single component, it can improve the control effect on guava anthracnose. In addition, the fungicidal composition of the present application is compounded by rock lily lactone and berberine or pydiflumetofen, which can delay the development and development of pathogen resistance compared with a single component, and can prolong the service life of the pesticide.

Claims

1. A bactericidal composition for preventing and controlling anthracnose in passion fruit, characterized in that, Its active ingredient is composed of bergapten and berberine, wherein the mass ratio of bergapten to berberine is 1-7:15-1.

2. The bactericidal composition for controlling passion fruit anthracnose according to claim 1, characterized in that, The mass ratio of Bergenin to Berberine is 1:7.

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