Separation and purification method of bacillus amyloliquefaciens antimicrobial proteins

A technology of amylolytic spores and antibacterial proteins, which is applied in peptide preparation methods, chemical instruments and methods, and organic chemistry. The effect of bacterial activity
CN102153618AInactive Publication Date: 2011-08-17INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
1 Cites 17 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
Publication Date
2011-08-17
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a separation and purification method of bacillus amyloliquefaciens antimicrobial proteins, relating to a separation and purification method of bacillus antimicrobial proteins. The method solves the problems that the existing separation and purification method of proteins can not acquire bacillus amyloliquefaciens TF28 antimicrobial proteins with high purity, generates various impurity proteins and is low in the yield of antimicrobial proteins. The method includes the following steps: I. obtaining the crude extract of antimicrobial proteins; II. obtaining protein concentrate by anion exchange chromatography; and III. performing gel filtration chromatography through a molecular sieve to obtain bacillus amyloliquefaciens antimicrobial proteins. The separation and purification method is used for the separation and purification of bacillus amyloliquefaciens antimicrobial proteins.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a separation and purification method of bacillus antibacterial protein. Background technique

[0002] Antibacterial proteins show strong antibacterial activity. In addition, antibacterial proteins can form ion channels on the bacterial plasma membrane, destroy the membrane potential, cause intracellular material leakage and kill bacteria, so it is not easy to produce drug resistance and cross resistance. Become the main research direction of new biopesticides.

[0003] Although many bio-control microorganisms can secrete antibacterial proteins, and the separation and purification methods of proteins have been developed relatively well, but due to different strains, the properties of antibacterial proteins are very different, and the purification methods are also different.

[0004] Bacillus amyloliquefaciens TF28 (Bacillus amyloliquefaciens TF28) belongs to the genus Bacillus, its preservation number is CGMCC No.4038, and its ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More