Anti-diarrhea feed additive and preparation method thereof

Abstract

The invention discloses an anti-diarrhea feed additive and a preparation method thereof, and belongs to the technical field of feed additives. The anti-diarrhea feed additive is prepared from 5 to 10 parts of phloretin, 3 to 5 parts of creatine, 10 to 15 parts of methyl cinnamate, 10 to 15 parts of ethyl cinnamate, 10 to 15 parts of propyl cinnamate, 10 to 15 parts of butyl cinnamate, 20 to 30 parts of montmorillonite, 10 to 15 parts of inulin, 6 to 10 parts of hydrogenated vegetable oil and 3 to 5 parts of sodium alginate. According to the anti-diarrhea feed additive and the preparation method thereof, the phloretin, the creatine, the cinnamate, the montmorillonite and the inulin are scientifically compounded, so that the anti-diarrhea effect of the feed additive is remarkably improved, meanwhile, intestinal health can be effectively guaranteed, nutrient absorption is promoted, and the growth performance of livestock and poultry is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of feed additives, in particular to an anti-diarrhea feed additive and a preparation method thereof. BACKGROUND

[0002] In the current livestock and poultry breeding industry, diarrhea is a common and harmful disease, especially in the weaning stage of piglets. Antibiotics or zinc oxide are commonly used as prevention and treatment measures in farms, but long-term abuse of antibiotics can easily lead to increased bacterial drug resistance and drug residues, and the use of high-dose zinc oxide has the risk of environmental pollution and accumulation in the animal body, so its application is limited. Therefore, it is an urgent need in the industry to develop a new type of green feed additive that is safe, efficient and pollution-free. Some existing natural plant extracts or mineral additives have certain effects, but they often have the shortcomings of single composition, limited target, easy inactivation in gastric acid environment or unstable effect, which leads to poor comprehensive effect of anti-diarrhea and is difficult to meet the overall requirements of large-scale breeding for intestinal health and growth performance of livestock and poultry. SUMMARY

[0003] The purpose of the present application is to provide an anti-diarrhea feed additive and a preparation method thereof, which realizes comprehensive prevention and control of viruses and bacteria by scientific compounding of phloretin, creatine, cinnamic acid ester compounds, montmorillonite and inulin, significantly improves the anti-diarrhea effect of the feed additive, effectively protects the intestinal health, promotes nutrient absorption, and thus improves the growth performance of livestock and poultry.

[0004] To achieve the above purpose, the present application provides an anti-diarrhea feed additive, which comprises phloretin 5-10 parts, creatine 3-5 parts, methyl cinnamate 10-15 parts, ethyl cinnamate 10-15 parts, propyl cinnamate 10-15 parts, butyl cinnamate 10-15 parts, montmorillonite 20-30 parts, inulin 10-15 parts, hydrogenated vegetable oil 6-10 parts and sodium alginate 3-5 parts.

[0005] Preferably, the hydrogenated vegetable oil comprises hydrogenated corn oil, hydrogenated soybean oil or hydrogenated rapeseed oil.

[0006] The present application also provides a preparation method of the anti-diarrhea feed additive, comprising the following steps: S1, mixing hydrogenated vegetable oil and sodium alginate, heating and stirring to obtain a molten liquid; S2, mixing phloretin, creatine, montmorillonite and inulin, passing through a 60-80 mesh sieve, and adding the molten liquid to obtain a mixed matrix; S3, adding methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix and stirring to obtain a mixed liquid; S4. Homogenize the mixture, cool and spray granulate to obtain an anti-diarrheal feed additive.

[0007] Preferably, in S1, the stirring temperature is 70-85℃, the stirring speed is 200-400rpm, and the stirring time is 10-20min.

[0008] Preferably, in S2, the stirring temperature is 70-85℃, the stirring speed is 300-500 rpm, and the stirring time is 15-25 min.

[0009] Preferably, in S3, the stirring temperature is 70-85℃, the stirring speed is 200-400rpm, and the stirring time is 10-20min.

[0010] Preferably, in S4, the homogenization speed is 3500-4500 rpm and the homogenization time is 15-30 min.

[0011] Preferably, in S4, the temperature of the cooling spray granulation is 5-15℃, and the flow rate of the cooling spray granulation is 5-15mL / min.

[0012] The present invention also provides an anti-diarrheal feed, comprising the above-mentioned anti-diarrheal feed additive, wherein each ton of feed contains 1-3 kg of the above-mentioned anti-diarrheal feed additive.

[0013] Therefore, the present invention, employing the above-mentioned anti-diarrheal feed additive and its preparation method, has the following beneficial effects: (1) The antidiarrheal feed additive provided by the present invention significantly improves the antidiarrheal effect of the feed additive by scientifically compounding phloretin, creatine, cinnamic acid ester, montmorillonite and inulin, while effectively protecting intestinal health and promoting nutrient absorption, thereby improving the growth performance of livestock and poultry.

[0014] (2) The antidiarrheal feed additive provided by this invention contains phlorizin and creatine, which have inhibitory effects on different types of viruses. The two work synergistically to achieve comprehensive prevention and control of viruses. Cinnamic acid esters (methyl ester, ethyl ester, propyl ester, butyl ester) have broad-spectrum antibacterial and anti-inflammatory effects, which can effectively kill or inhibit pathogenic bacteria in the intestine (such as Escherichia coli and Salmonella) and alleviate intestinal inflammatory reactions. Therefore, the synergistic effect of phlorizin, creatine and cinnamic acid esters significantly reduces the rate of viral and bacterial diarrhea in livestock and poultry.

[0015] (3) The montmorillonite in the anti-diarrheal feed additive provided by this invention has a layered structure and non-uniform charge distribution, which can physically adsorb bacteria, viruses, toxins and their generated gases in the intestine, and cover the digestive tract mucosa, protecting the mucosal barrier and creating conditions for creatine and cinnamic acid esters to play their role; creatine provides energy to repair the damaged intestinal mucosa. The three work synergistically to not only stop diarrhea quickly, but also fundamentally repair intestinal damage, prevent recurrence of diarrhea, and significantly improve the average daily weight gain of livestock and poultry.

[0016] (4) The inulin in the antidiarrheal feed additive provided by the present invention is a high-quality prebiotic that can reach the colon directly without being digested by the gastrointestinal tract and be specifically utilized by beneficial bacteria such as Bifidobacterium, thereby regulating the balance of intestinal flora, improving the body's immunity and digestive and absorptive capacity, thereby achieving the goal of clearing pathogens with phloretin, creatine and cinnamic acid esters, while improving the intestinal microenvironment and increasing the feed conversion rate, further promoting the growth and development of livestock and poultry.

[0017] (5) The hydrogenated vegetable oil in the antidiarrheal feed additive provided by the present invention has thickening, hardening and sustained-release effects, and sodium alginate has good film-forming and gelling abilities. The two are used together as formulation excipients to encapsulate the active ingredients. Cinnamyl esters and phloretin are unstable in the gastric acid environment and are easily decomposed and inactivated. The present invention utilizes hydrogenated vegetable oil and sodium alginate to form a dense sustained-release coating layer on the surface of cinnamyl esters and phloretin, thereby effectively blocking the erosion of gastric acid, allowing the active ingredients to pass smoothly through the stomach to the intestines and be released in a targeted manner in the intestines, which greatly improves the bioavailability and antidiarrheal effect of the drug.

[0018] The technical solution of the present invention will be further described in detail below through embodiments. Detailed Implementation

[0019] The technical solution of the present invention will be further described below through embodiments.

[0020] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0021] In this invention, unless otherwise specified, the test materials and instruments are all conventional test materials in the field and can be purchased through commercial channels.

[0022] Example 1 An anti-diarrheal feed additive comprises 5 parts phloretin, 4 parts creatine, 15 parts methyl cinnamate, 10 parts ethyl cinnamate, 13 parts propyl cinnamate, 15 parts butyl cinnamate, 20 parts montmorillonite, 12 parts inulin, 10 parts hydrogenated corn oil (hydrogenated vegetable oil), and 3 parts sodium alginate.

[0023] The preparation method of the above-mentioned anti-diarrheal feed additive includes the following steps: S1. Place hydrogenated corn oil and sodium alginate in an emulsification tank, mix, heat, and stir at 200 rpm for 20 minutes at 80°C to completely melt and mix evenly to obtain a molten liquid. S2. Mix phloretin, creatine, montmorillonite and inulin, pass through an 80-mesh sieve to ensure no lumps, add to the molten liquid, and stir at 400 rpm for 20 minutes at 80°C to obtain a mixed matrix. S3. Add methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix, and stir at 300 rpm for 15 min at 80°C to increase the fineness and stability, and obtain the mixture. S4. Homogenize the mixture at 4000 rpm for 20 min, then pump it into a cooling spray granulation tower via a transfer pump. Perform cold spray granulation at a temperature of 10℃ and a flow rate of 10 mL / min to obtain an anti-diarrheal feed additive.

[0024] Example 2 An anti-diarrheal feed additive comprises 8 parts phloretin, 5 parts creatine, 10 parts methyl cinnamate, 12 parts ethyl cinnamate, 15 parts propyl cinnamate, 10 parts butyl cinnamate, 25 parts montmorillonite, 15 parts inulin, 6 parts hydrogenated soybean oil (hydrogenated vegetable oil), and 4 parts sodium alginate.

[0025] The preparation method of the above-mentioned anti-diarrheal feed additive includes the following steps: S1. Place hydrogenated soybean oil and sodium alginate in an emulsification tank, mix, heat, and stir at 300 rpm for 15 minutes at 70°C to completely melt and mix evenly to obtain a molten liquid. S2. Mix phloretin, creatine, montmorillonite and inulin, pass through an 80-mesh sieve to ensure no lumps, add to the molten liquid, and stir at 500 rpm for 15 minutes at 85°C to obtain a mixed matrix. S3. Add methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix, and stir at 300 rpm for 15 min at 80°C to increase the fineness and stability, and obtain the mixture. S4. Homogenize the mixture at 4000 rpm for 20 min, then pump it into a cooling spray granulation tower via a transfer pump. Perform cold spray granulation at a temperature of 10℃ and a flow rate of 10 mL / min to obtain an anti-diarrheal feed additive.

[0026] Example 3 An anti-diarrheal feed additive comprises 10 parts phloretin, 3 parts creatine, 12 parts methyl cinnamate, 15 parts ethyl cinnamate, 10 parts propyl cinnamate, 13 parts butyl cinnamate, 30 parts montmorillonite, 10 parts inulin, 8 parts hydrogenated rapeseed oil (hydrogenated vegetable oil), and 5 parts sodium alginate.

[0027] The preparation method of the above-mentioned anti-diarrheal feed additive includes the following steps: S1. Place hydrogenated rapeseed oil and sodium alginate in an emulsification tank, mix, heat, and stir at 200 rpm for 20 minutes at 85°C to completely melt and mix evenly to obtain a molten liquid. S2. Mix phloretin, creatine, montmorillonite and inulin, pass through an 80-mesh sieve to ensure no lumps, add to the molten liquid, and stir at 500 rpm for 15 minutes at 70°C to obtain a mixed matrix. S3. Add methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix, and stir at 400 rpm for 10 min at 70°C to increase the fineness and stability to obtain a mixture. S4. Homogenize the mixture at 4000 rpm for 20 min, then pump it into a cooling spray granulation tower via a transfer pump. Perform cold spray granulation at a temperature of 10℃ and a flow rate of 10 mL / min to obtain an anti-diarrheal feed additive.

[0028] Example 4 An anti-diarrheal feed additive comprises 6 parts phloretin, 5 parts creatine, 10 parts methyl cinnamate, 15 parts ethyl cinnamate, 12 parts propyl cinnamate, 13 parts butyl cinnamate, 22 parts montmorillonite, 15 parts inulin, 10 parts hydrogenated corn oil (hydrogenated vegetable oil), and 3 parts sodium alginate.

[0029] The preparation method of the above-mentioned anti-diarrheal feed additive includes the following steps: S1. Place hydrogenated corn oil and sodium alginate in an emulsification tank, mix, heat, and stir at 200 rpm for 20 minutes at 80°C to completely melt and mix evenly to obtain a molten liquid. S2. Mix phloretin, creatine, montmorillonite and inulin, pass through an 80-mesh sieve to ensure no lumps, add to the molten liquid, and stir at 400 rpm for 20 minutes at 80°C to obtain a mixed matrix. S3. Add methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix, and stir at 200 rpm for 20 min at 85°C to increase the fineness and stability to obtain a mixture. S4. Homogenize the mixture at 4000 rpm for 20 min, then pump it into a cooling spray granulation tower via a transfer pump. Perform cold spray granulation at a temperature of 10℃ and a flow rate of 10 mL / min to obtain an anti-diarrheal feed additive.

[0030] Comparative Example 1 An anti-diarrheal feed additive comprises 5 parts phloretin, 15 parts methyl cinnamate, 10 parts ethyl cinnamate, 13 parts propyl cinnamate, 15 parts butyl cinnamate, 20 parts montmorillonite, 12 parts inulin, 10 parts hydrogenated corn oil (hydrogenated vegetable oil), and 3 parts sodium alginate.

[0031] The preparation method of the above-mentioned anti-diarrheal feed additive includes the following steps: S1. Place hydrogenated corn oil and sodium alginate in an emulsification tank, mix, heat, and stir at 200 rpm for 20 minutes at 80°C to completely melt and mix evenly to obtain a molten liquid. S2. Mix phloretin, montmorillonite, and inulin, pass through an 80-mesh sieve to ensure there are no lumps, add to the molten liquid, and stir at 400 rpm for 20 minutes at 80°C to obtain a mixed matrix. S3. Add methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix, and stir at 300 rpm for 15 min at 80°C to increase the fineness and stability, and obtain the mixture. S4. Homogenize the mixture at 4000 rpm for 20 min, then pump it into a cooling spray granulation tower via a transfer pump. Perform cold spray granulation at a temperature of 10℃ and a flow rate of 10 mL / min to obtain an anti-diarrheal feed additive.

[0032] Comparative Example 2 An anti-diarrheal feed additive comprises 5 parts phloretin, 4 parts creatine, 15 parts methyl cinnamate, 10 parts ethyl cinnamate, 13 parts propyl cinnamate, 15 parts butyl cinnamate, 20 parts montmorillonite, and 12 parts inulin.

[0033] The preparation method of the above-mentioned anti-diarrheal feed additive includes the following steps: S1. Mix phloretin, creatine, montmorillonite and inulin, pass through an 80-mesh sieve to ensure no lumps, and then stir at 400 rpm for 20 minutes at 80°C to obtain a mixed matrix. S2. Add methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix, and stir at 300 rpm for 15 minutes at 80°C to increase fineness and stability, thereby obtaining an anti-diarrheal feed additive.

[0034] Comparative Example 3 An anti-diarrheal feed additive comprises 5 parts phloretin, 4 parts creatine, 15 parts methyl cinnamate, 10 parts ethyl cinnamate, 13 parts propyl cinnamate, 15 parts butyl cinnamate, 20 parts montmorillonite, 12 parts corn starch, 10 parts hydrogenated corn oil (hydrogenated vegetable oil), and 3 parts sodium alginate.

[0035] The preparation method of the above-mentioned anti-diarrheal feed additive includes the following steps: S1. Place hydrogenated corn oil and sodium alginate in an emulsification tank, mix, heat, and stir at 200 rpm for 20 minutes at 80°C to completely melt and mix evenly to obtain a molten liquid. S2. Mix phloretin, creatine, montmorillonite and corn starch, pass through an 80-mesh sieve to ensure no lumps, add to the molten liquid, and stir at 400 rpm for 20 minutes at 80°C to obtain a mixed matrix. S3. Add methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate to the mixed matrix, and stir at 300 rpm for 15 min at 80°C to increase the fineness and stability, and obtain the mixture. S4. Homogenize the mixture at 4000 rpm for 20 min, then pump it into a cooling spray granulation tower via a transfer pump. Perform cold spray granulation at a temperature of 10℃ and a flow rate of 10 mL / min to obtain an anti-diarrheal feed additive.

[0036] Forty hundred healthy weaned piglets aged 28 days were randomly divided into eight groups of 50 each, numbered 1-8. Groups 1-7 were the experimental groups, and group 8 was the control group. During the experiment, the piglets had free access to feed and water. The control group was fed a basal diet, while the experimental groups received 1 kg of anti-diarrheal feed additive per ton of basal diet. The amounts of anti-diarrheal feed additives added to different groups are shown in Table 1. Feeding continued for 14 days. The average weight of each group was recorded at the beginning and end of the experiment. The number of piglets with diarrhea, average weight, and daily feed intake were recorded daily for each group. After the experiment, the diarrhea rate, average daily weight gain, and average daily feed intake for each group were calculated.

[0037] Table 1. Antidiarrheal feed additives added to each group

[0038] Table 2. Diarrhea and growth status of piglets in each group

[0039] As shown in Table 2, the anti-diarrheal feed additive provided by this invention exhibits significant advantages in reducing the diarrhea rate and improving the growth performance of piglets. The average daily weight gain of the blank control group (groups 8) was 186 g / day per head, and the diarrhea rate was 18.8%, indicating that the piglets were in a state of severe growth inhibition and impaired health. Compared with group 8, groups 5, 6, and 7 showed increased average daily weight gain and decreased diarrhea rate, but were still significantly higher than groups 1-4. The anti-diarrheal feed additives in group 5, lacking creatine, could not inhibit non-enveloped viruses such as rotavirus, slowing down intestinal repair in piglets and narrowing the antiviral spectrum. The anti-diarrheal feed additive in group 6, without coating, had the worst anti-diarrheal effect, indicating that without the protective layer formed by hydrogenated vegetable oil and sodium alginate, cinnamic acid esters and phloretin are destroyed in the stomach and cannot reach the intestines to exert their effects, leading to drug ineffectiveness. The anti-diarrheal feed additive in group 7, using corn starch instead of inulin, had a slightly lower anti-diarrheal effect and daily weight gain than groups 1-4, indicating that inulin, as a prebiotic, can promote nutrient absorption and improve feed conversion rate by regulating intestinal flora. Based on this, after adding the anti-diarrheal feed additive of this invention, the average daily weight gain of groups 1-4 increased to 295-302 g / day per head, and the diarrhea rate decreased to 1.8%-2.2%, indicating that the anti-diarrheal feed additive of this invention has a significant effect in reducing diarrhea in livestock and poultry.

[0040] Therefore, the present invention adopts the above-mentioned anti-diarrheal feed additive and its preparation method. By scientifically compounding phloretin, creatine, cinnamic acid ester, montmorillonite and inulin, the anti-diarrheal effect of the feed additive is significantly improved. At the same time, it can effectively protect intestinal health, promote nutrient absorption, and thus improve the growth performance of livestock and poultry.

[0041] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. An anti-diarrhea feed additive, characterized by: Phloretin 5-10 parts, creatine 3-5 parts, methyl cinnamate 10-15 parts, ethyl cinnamate 10-15 parts, propyl cinnamate 10-15 parts, butyl cinnamate 10-15 parts, montmorillonite 20-30 parts, inulin 10-15 parts, hydrogenated vegetable oil 6-10 parts, sodium alginate 3-5 parts.

2. The anti-diarrhea feed additive according to claim 1, characterized in that: The hydrogenated vegetable oil includes hydrogenated corn oil, hydrogenated soybean oil or hydrogenated rapeseed oil.

3. A process for the preparation of an antidiarrheal feed additive according to any one of claims 1-2, characterized by: The method comprises the following steps: S1, hydrogenated vegetable oil, sodium alginate are mixed, heated and stirred to obtain a molten liquid; S2, phloretin, creatine, montmorillonite and inulin are mixed, passed through a 60-80 mesh sieve, and added to the molten liquid, stirred to obtain a mixed matrix; S3, methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate are added to the mixed matrix, stirred to obtain a mixed liquid; S4, the mixed liquid is homogenized, cooled and spray granulated to obtain an anti-diarrhea feed additive.

4. A method of preparing an anti-diarrhea feed additive according to claim 3, characterized in that: In S1, the stirring temperature is 70-85°C, the stirring speed is 200-400 rpm, and the stirring time is 10-20 min.

5. A method of preparing an anti-diarrhea feed additive according to claim 3, characterized in that: In S2, the stirring temperature is 70-85°C, the stirring speed is 300-500 rpm, and the stirring time is 15-25 min.

6. A method of preparing an anti-diarrhea feed additive according to claim 3, characterized in that: In S3, the stirring temperature is 70-85°C, the stirring speed is 200-400 rpm, and the stirring time is 10-20 min.

7. The method for preparing an anti-diarrheal feed additive according to claim 3, characterized in that: In S4, the homogenization speed is 3500-4500 rpm, and the homogenization time is 15-30 min.

8. A method of preparing an anti-diarrhea feed additive according to claim 3, characterized by: In S4, the cooling and spray granulation temperature is 5-15°C, and the cooling and spray granulation flow rate is 5-15 mL / min.

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