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Employment of rotavirus proteins, derived proteins and peptides for the modulation of tisssue permeability

a permeability and tissue technology, applied in the direction of viruses, fusion polypeptides, macromolecular non-active ingredients, etc., can solve the problems of morbidity and mortality, the method has been largely inefficient, etc., and achieve the effect of enhancing the passage of therapeutic agents and enhancing the delivery of drugs

Inactive Publication Date: 2006-11-09
CENT DE INVESTIGACION & DE ESTUDIOS AVANZADOS DEL INST POLITECNICO NACIONAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0036] Another aspect of the present invention is the use of rotavirus protein VP4 or its derived polypeptide VP8 or peptides derived from them to increase the paracellular permeability of epithelia and endothelia.
[0038] One additional aspect of the present invention is the use of rotavirus protein VP4, its derived polypeptide VP8, or peptides derived from them, to modulate and / or enhance the passage of therapeutical agents through the intestinal, nasal, ocular, vaginal and rectal epithelium.
[0040] Within another aspect of the present invention, is the use of rotavirus protein VP4, its derived polypeptide VP8, or peptides derived from them, to allow and / or to enhance the passage of therapeutical agents across the blood-brain barrier.
[0041] Within a further aspect of the present invention, is to enhance the delivery of a drug to a tumor in a mammal, comprising administering the rotavirus protein VP4, its derived polypeptide VP8 or peptides derived from them, in combination with a drug to a tumor-bearing mammal.
[0045] Within another related aspect of the present invention, protein VP4, its derived polypeptide VP8, or peptides derived from them can be used to reduce unwanted cellular adhesion that can occur between tumor cells, tumor cells and normal cells or between normal cells as a result of surgery, injury, chemotherapy, disease, inflammation or other condition jeopardizing cell viability or function.

Problems solved by technology

Such situation is encountered when the barrier formed by the epithelia or endothelia creates difficulties for the delivery of drugs to specific tissues and tumors within the body.
However such methods have been largely inefficient due to low endogenous transport rates or to their poor functioning with applied drugs.
Rotaviruses are the leading cause of morbidity and mortality caused by gastroenteritis in children less than 2 years old.

Method used

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  • Employment of rotavirus proteins, derived proteins and peptides for the modulation of tisssue permeability
  • Employment of rotavirus proteins, derived proteins and peptides for the modulation of tisssue permeability
  • Employment of rotavirus proteins, derived proteins and peptides for the modulation of tisssue permeability

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0127] Effect of rotavirus protein VP8 on the degree of sealing of epithelial monolayers.

[0128] This example illustrates an assay for evaluating the effect of rotavirus proteins on transepithelial electrical resistance.

[0129] A) VP8 Cloning [0130] The RRV VP8 fragment (VP4 gene nucleotides 1 to 750) was cloned in pGEX-4T-1 (Pharmacia) and in pET6HIS plasmids as previously described (Dowling et al., 2000; Isa et al., 1997).

[0131] B) VP5 Cloning [0132] The RRV VP5 fragment (VP4 gene nucleotides 749 to 2347) was cloned in pGEX4T-2 (Pharmacia) as previously described (Zarate et al., 2000b).

[0133] C) Expression, Purification, and Administration of Fusion Proteins

[0134] Expression and purification of fusion proteins was performed following previously described standard procedures (Frangioni et al., 1993). For their experimental use, fusion proteins VP5 and VP8 were dissolved in fresh Dulbecco's minimal essential medium (DMEM) and sterilized by passage through a 0.22 μm filtration uni...

example 2

[0141] VP8 modifies the cell border distribution of TJ proteins.

[0142] This example illustrated an epithelial cell assay for evaluating the effect of VP8 on the distribution of TJ proteins.

[0143] A) Immunolocalization of TJ Proteins.

[0144] MDCK cells were cultured in glass coverslips. Confluent cultures were exposed to 4 μg / ml of GST-VP8 for 1 hr. The cells were then fixed for 30 min with 2% p-formaldehyde in PBS, pH 7.4, and permeabilized for 3 min with 0.2% PBS-TX. Cells were washed five times with PBS and then blocked for 30 minutes with 1% BSA Ig free (Research Organics 1331-a). The monolayers were incubated overnight at 4° C. with rabbit polyclonal antibodies against ZO-1 (Zymed 61-7300, dilution 1:100), Claudin 3 (Zymed 34-1700, dilution 3 lgr / ml) or occludin (Zymed 71-1500, dilution 1:100 in 1% Ig-free BSA). After being washed five times with PBS, the coverslips were incubated for one hour at room temperature with a secondary antibody (FITC-conjugated goat anti-rabbit, cat...

example 3

[0146] Effect of VP8 on the freeze fracture appearance of TJ.

[0147] This example illustrates how the pattern of TJ filaments is modified by VP8.

[0148] A)Freeze Fracture Analysis of TJ.

[0149] Confluent monolayers of MDCK cells were incubated for 1 hour with 4 μg / ml of GST-VP8 dissolved in DMEM, while control monolayers remained in DMEM. Freeze-fracture replicas were obtained from monolayers fixed with 2.5% glutaraldehyde for 30 minutes, and gradually infiltrated with glycerol up to a 20% concentration, in which they were left for 1 hour. The monolayers were then detached from the substrate and frozen in liquid nitrogen. Freeze fracture was carried out at −120° C., and 2 x 10-6 mm Hg using a Balzers apparatus (BAF400T). After evaporation of platinum and carbon, the organic material was digested for 1 hour in chromic mixture. Replicas were extensively washed in distilled water and mounted in Formvar coated grids. The observations were done in an electron microscope JEOL 200EX.

[0150...

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Abstract

The present invetnion refers to the use of rotavirus proteins VP4, VP8 and their derived fusion proteins and peptides, for enhancing the delivery of pharmaceutical agents through the paracellular pathway. These rotavirus proteins and derived peptides may additionally be employed to reduce unwanted cellular adhesion that can occur between cancerous cells, or between normal cells as a result of surgery, injury, chemotherapy, disease, inflammation or other pathological conditions.

Description

TECHNICAL FIELD [0001] The present invention relates generally to methods for regulating tight junction sealing, and more particularly to the use of proteins and peptides that inhibit cell adhesion and the formation of tissue permeability barriers. BACKGROUND OF THE INVENTION The Tight Junction (TJ) [0002] In multicellular organisms fluids with different molecular compositions (urine, milk, gastric juice, blood etc.) are contained in compartments delineated by epithelia (e.g. renal tubules) and endothelia (blood vessels). These cellular sheets constitute the frontier between the organism internal milieu and the compartments' contents. Therefore in order for components of the blood to enter a given tissue, they must first traverse from the lumen of the blood vessel through the endothelial cells of that vessel. In case of substances that enter the body via the gut, they must first pass the barrier formed by the epithelial cells that line the cavity, and to enter the blood via the ski...

Claims

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Application Information

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IPC IPC(8): A61K38/28C12Q1/70A61K39/42C07H21/04A61K31/704A61K31/43A61K39/15C07K14/005C07K16/10A61K38/16A61K47/42A61P35/00C07K14/14C12N7/01
CPCA61K38/162A61K47/42C12N2720/12322C07K2319/00C07K14/005A61P3/10A61P35/00Y02A50/30C07K14/14
Inventor MARISCAL-GONZALEZ, LORENZANAVA-DOMINGUEZ, PORFIRIO
Owner CENT DE INVESTIGACION & DE ESTUDIOS AVANZADOS DEL INST POLITECNICO NACIONAL
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