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Attenuated vaccine strain for avian infectious bronchitis virus and application thereof

A technology for bronchitis and chicken infectivity, applied in the directions of antiviral agent, inactivation/attenuation, virus/phage, etc., can solve the problem of inability to effectively prevent the attack of IB epidemic strains, and achieve the effect of good safety

Inactive Publication Date: 2013-01-02
SHANGHAI QISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to the Mass-type serotype, there are quite a few of the current domestic epidemic strains that are quite different from the Mass-type vaccine strains in terms of genetic evolution. Therefore, the existing vaccines in my country cannot effectively prevent the attack of IB epidemic strains.

Method used

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  • Attenuated vaccine strain for avian infectious bronchitis virus and application thereof
  • Attenuated vaccine strain for avian infectious bronchitis virus and application thereof
  • Attenuated vaccine strain for avian infectious bronchitis virus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0129] Preparation of attenuated strain

[0130] The IBV-QD01 virus strain (purchased from Shanghai Qisheng Biotechnology Co., Ltd.) was continuously subcultured using SPF chicken embryos aged 9 to 11 days, and the allantoic cavity of 2 to 3 chicken embryos was inoculated in each generation, and 100 ~200μl, placed at 37.8℃, humidity 45%~60%, cultured for 18~48 hours, aseptically collected the allantoic fluid of chicken embryos, and carried out the next subculture, and carried out cloning after continuous passage for more than 70 generations, and measured the virus titer And after attenuation evaluation, 6 ideal clones were obtained.

[0131] Chicken embryos are inoculated after the virus is collected, further amplified for one generation, and the allantoic fluid is aseptically collected as the virus material for the next animal test.

[0132] A preferred clone is chicken infectious bronchitis attenuated vaccine strain IBV-KL05 (Infectious Bronchitis Virus, IBV), which was dep...

Embodiment 2

[0135] Immunoprotective effect of attenuated strain

[0136] In this example, the immune challenge protection test of the IBV-KL05 strain against different virulent serotypes was tested. Methods as below:

[0137] In the experiment, 80 1-day-old SPF chicks were divided into five groups.

[0138] Groups 1-2: 20 rats in each group, inoculated with IBV-KL05MVS+3 by eye drops, 10 rats in each 3.5 EID 50 ;

[0139] Group 3: 20 rats, inoculated with IBV-H120 vaccine, 10 rats each 3.5 EID50 ;

[0140] Groups 4-5 were the normal control group, with 10 rats in each group, without inoculation.

[0141] Three weeks after inoculation, the virus was challenged. The strong poison used for the first, third and fourth groups was IBV-QD01, and the strong poison for the second and fifth groups was IBV-M41. Eye drops and nasal drops were used for the challenge. Dosage is 10 each 4.5 EID 50 .

[0142] Clinical symptoms were observed daily. Six days after the challenge, the cilia stagna...

Embodiment 3

[0150] Safety Test of Attenuated Strain on Female Reproductive System

[0151] The test took 51 1-day-old female SPF chicks and divided them into two groups:

[0152] The first group of 46 rats, each eye was inoculated with 10 5.5 EID 50 IBV-KL05 MVS;

[0153] The other group of 5 rats was the normal control group without inoculation. Chickens were observed at least once a day.

[0154] At the age of 11 weeks, all the chickens were dissected, and the fallopian tubes were observed for abnormalities such as cysts, deformation, hypoplasia, etc.

[0155] Results: Four chicks in the first group died 20 days after immunization due to bacterial infection of the yolk sac or pecking.

[0156] One chick in the second group was culled from this group on day 8 post-immunization due to stunting. The 42 test group chickens and 4 control group chickens that survived the experiment were dissected 11 weeks after inoculation, and no abnormalities were found in the oviducts.

[0157] The...

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Abstract

The method relates to an attenuated vaccine strain for avian infectious bronchitis virus and application thereof. According to the invention, avian infectious bronchitis virus (IBV) is attenuated, so as to successfully obtain an IBV attenuated strain CCTCC.V201232 and a derivative virus strain thereof. The attenuated strain and derivative virus strain provided by the invention can be used in preparation of a vaccine composition for prevention of infectious bronchitis. Experiments show that the attenuated strain and vaccine composition provided by the invention can be inoculated to immature birds, so as to effectively activate immune system in the birds and well prevent avian infectious bronchitis.

Description

technical field [0001] The present invention relates to the fields of bioengineering and immunization, specifically, an attenuated strain of chicken infectious bronchitis virus (Infectious Bronchitis virus, IBV), a derivative strain of the attenuated strain, and its IBV prevention and control vaccination and vaccine composition application in preparation. Background technique [0002] Chicken infectious bronchitis (Avian Infectious Bronchitis, IB) is an acute, highly contagious infectious disease caused by IBV (Infectious Bronchitis virus, IBV). IBV natural infection mainly occurs in chickens, and chickens of all ages are susceptible. IBV mainly affects the respiratory tract, kidneys and oviducts of chickens. Clinical symptoms are more common in chickens less than six weeks old, and the infection rate is high, but the mortality rate is low. After broiler chickens are infected, the food conversion rate is mainly reduced, the weight gain is slow, and secondary infection may...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/215A61P31/14C12N7/08C12R1/93
Inventor 侯艳霞张超董文龙寇芮
Owner SHANGHAI QISHENG BIOTECH CO LTD
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