Vaccine strains of infectious clones of porcine reproductive and respiratory syndrome virus (PRRSV) and application thereof

A technology for respiratory syndrome and pig breeding, applied in the field of bioengineering, can solve problems such as large immunization dose of inactivated vaccines, unsatisfactory immunization effect of heterologous strains, safety concerns, etc., and achieve an effect that is conducive to prevention and control
CN101984061AActive Publication Date: 2011-03-09SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
Publication Date
2011-03-09

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Abstract

The invention discloses an artificially cloned attenuated vaccine strains. The vaccine strains are strains cloned from attenuated vaccine strains HuN4-F112 of highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV), and marked restriction enzyme sites are introduced in the structural protein gene sequences of the strains. The invention also discloses a recombinant vector which comprises a full-length gene cDNA sequence of the attenuated vaccine strains HuN4-F112 of the highly pathogenic PRRSV. The 5'-end of the full-length gene cDNA sequence is additionally provided witha transcription promoter and the full-length gene cDNA sequence is internally introduced with the marked restriction enzyme sites. The artificially cloned attenuated vaccine strains of the invention can not only provide completely safe immune protection for resistance of an immune pig to the highly pathogenic PRRSV, but also effectively distinguish an immune pig of the PRRSV from a naturally infectious pig of the PRRSV, thus being beneficial to preventing and controlling the highly pathogenic PRRSV.
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Description

Technical field

[0001] The invention relates to the technical field of bioengineering, in particular to an artificially cloned attenuated vaccine strain with infectious and highly pathogenic porcine reproductive and respiratory syndrome virus and its application. Background technique

[0002] Porcine reproductive and respiratory syndrome (PRRS) is caused by the PRRS virus (PRRSV) due to pregnancy sows abortion, stillbirth, weak fetus, mummified fetus and other reproductive disorders, and respiratory symptoms and symptoms of pigs at various ages. An infectious disease characterized by high mortality, commonly known as "Porcine Reproductive and Respiratory Syndrome", is one of the seven major swine diseases legally reported by OIE. Since it was first discovered in 1996, it has been popular in my country, causing huge economic losses to the pig industry in my country. In 2006, a "swine hyperthermia syndrome" spread rapidly in our pig herd, with an incidence rate of 50-100% and a mo...

Claims

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