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54 results about "Structural Protein Gene" patented technology
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A gene that encodes a protein with a chief function as part of a physical structure within a cell. This is in contrast to genes that encode proteins with a function that is enzymatic or regulatory.
Avirulent, immunogenic flavivirus chimeras
InactiveUS7094411B2Minimize and inhibit infectionStable maintenanceOrganic active ingredientsVirusesViral diseaseAmino acid mutation
Owner:MAHIDOL UNIV +2
Multi-epitope fusion diagnosis antigen for African swine fever virus as well as preparation method and application thereof
InactiveCN108148138AImprove featuresIncreased sensitivityAntibody mimetics/scaffoldsVirus peptidesAntigenBacillus coli
The invention discloses a multi-epitope fusion diagnosis antigen for African swine fever virus as well as a preparation method and application thereof. An ASFV (African swine fever virus) important structural protein gene encoding amino acid sequence is analyzed, screened and recombined through bioinformatics software, a multi-epitope fusion antigen gene is built and synthesized and is expressed in bacillus coli; through screening, the recombinant multi-epitope fusion antigen ASFV-meAg6 is obtained, so that diagnosis antigen protein with strong specificity and high sensitivity is provided foran ASFV serological diagnosis method.
Owner:SHIHEZI UNIVERSITY
Method for assembling foot and mouth disease virus hollow capsid in insect with acidproof improvement
The present invention discloses a method for assembling foot-and-mouth disease virus empty capsids in insect cells via the alteration of acid-resistance. The method for assembling foot-and-mouth disease virus empty capsids in insect cells includes the following steps: (1) the altered P12A gene and the non-structural protein gene 3C of foot-and-mouth disease virus are introduced into bacteria via baculovirus vectors for recombination to produce recombinant rhabdovirus A; (2) the DNA of the recombinant rhabdovirus A is used to transfect the insect cells, so that the foot-and-mouth disease virus empty capsids are obtained. The method assembles the integral foot-and-mouth disease virus empty capsids in the insect cells for the first time, lays a foundation for the research and the development of gene-engineered subunit vaccines and novel diagnostic reagents.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Avirulent, immunogenic flavivirus chimeras
InactiveUS20060062803A1Inhibition effectPreserve immunogenicityBiocideOrganic active ingredientsViral diseaseFhit gene
Chimeric flaviviruses that are avirulent and immunogenic are provided. The chimeric viruses are constructed to contain amino acid mutations in the nonstructural proteins of a flavivirus. Chimeric viruses containing the attenuation-mutated nonstructural genes of the virus are used as a backbone into which the structural protein genes of a second flavivirus strain are inserted. These chimeric viruses elicit pronounced immunogenicity yet lack the accompanying clinical symptoms of viral disease. The attenuated chimeric viruses are effective as immunogens or vaccines and may be combined in a pharmaceutical composition to confer simultaneous immunity against several strains of pathogenic flaviviruses.
Owner:MAHIDOL UNIV +2
Vaccine strains of infectious clones of porcine reproductive and respiratory syndrome virus (PRRSV) and application thereof
ActiveCN101984061AEasy to solveEasy to controlViral antigen ingredientsMicrobiological testing/measurementHighly pathogenicAttenuated vaccine
The invention discloses an artificially cloned attenuated vaccine strains. The vaccine strains are strains cloned from attenuated vaccine strains HuN4-F112 of highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV), and marked restriction enzyme sites are introduced in the structural protein gene sequences of the strains. The invention also discloses a recombinant vector which comprises a full-length gene cDNA sequence of the attenuated vaccine strains HuN4-F112 of the highly pathogenic PRRSV. The 5'-end of the full-length gene cDNA sequence is additionally provided witha transcription promoter and the full-length gene cDNA sequence is internally introduced with the marked restriction enzyme sites. The artificially cloned attenuated vaccine strains of the invention can not only provide completely safe immune protection for resistance of an immune pig to the highly pathogenic PRRSV, but also effectively distinguish an immune pig of the PRRSV from a naturally infectious pig of the PRRSV, thus being beneficial to preventing and controlling the highly pathogenic PRRSV.
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
siRNA capable of inhibiting foot and mouth disease virus replication and infection and its preparation method
InactiveCN1757725ATranscription assuranceEfficient transcriptionViruses/bacteriophagesAntibody medical ingredientsViral replicationDomestic animal
A kind of siRNAs able to suppress the copy and infection of foot-and-mouth disease virus in cell and animal individual and its preparing process and application are disclosed. The 1D protein gene and 3D polymerase gene are chosen as the interference targets from the genome of foot-and-mouth disease virus. The human recombinant adenovirus Ad5 is designed and used as the carrier of three siRNAs. The target of 21VPI siRNA and 63VPI siRNA is said 1D gene. The target of 56POL siRNA is 3D gene.
Owner:FUDAN UNIV
Encoding nucleotide sequence of codons optimizing rotavirus protein, recombinant and uses thereof
The invention pertains to the field of genetic engineering. More particularly, the invention relates to a coding nucleotide sequence of a codon optimized rotavirus VP6 or VP7 protein, a recombinant vector and a host cell which contain the sequence. The invention also relates to a method for optimizing and improving the amount of protein expression by the rotavirus structural protein gene codon and a usage on the immunology.
Owner:王健伟
Non-structural protein gene NS1 of avian influenza virus and its preparation method and use
The invention discloses the cDNA sequences of the non-structural protein gene NS1 of avian influenza virus, its preparation process and use, wherein gene is employed for constructing prokaryotic expression vectors, the expression vectors are transformed into bacillus coli to obtain recombinant strains (Escherichia coli BL21 / pET-28a-NS1), the strains are utilized to express the non-structural protein NS1 of the avian influenza virus, the expression proteins are used to establish a differential diagnosis method through enzyme linked immunosorbent assay for differentiate vaccinum avian influenzae inactivatum immune chicken from infected chicken. The invention also relates to a differential diagnosis reagent kit and its application.
Owner:HUAZHONG AGRI UNIV
CDNA (Complementary Deoxyribonucleic Acid) infectious clone of porcine reproductive and respiratory syndrome virus vaccine strain and application thereof
InactiveCN103695465AEasy to solveEasy to controlViral antigen ingredientsMicroorganism based processesComplementary deoxyribonucleic acidAttenuated vaccine
The invention provides a cDNA (complementary Deoxyribonucleic Acid) infectious clone of a porcine reproductive and respiratory syndrome virus vaccine strain and application thereof, and discloses a recombinant vector, which is an infectious clone of a porcine reproductive and respiratory syndrome virus vaccine strain CH1R constructed by adopting a reverse genetics approach. The infectious clone comprises a full-length gene cDNA sequence of the porcine reproductive and respiratory syndrome virus vaccine strain CH-1R. The invention further discloses an artificially cloned attenuated vaccine strain, which is a cloned strain of the porcine reproductive and respiratory syndrome virus vaccine strain CH-1R, wherein a marked restriction enzyme locus is introduced in a structural protein gene sequence. Through performing genetic engineering reconstruction of the infectious clone, a better vaccine can be researched, and the prevention and control of the porcine reproductive and respiratory syndrome are facilitated.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Method for efficiently producing recombinant avian adeno-associated virus
InactiveCN106085972AHigh titerSimple and fast operationPeptidesNucleic acid vectorFluorescenceTransfer vector
The invention discloses a method for efficiently producing recombinant avian adeno-associated virus. The method comprises the steps of first respectively constructing recombinant baculovirus rBacRep for expressing functional protein genes Rep78 and Rep52 of avian adeno-associated virus (AAAV), recombinant baculovirus rBacVP for expressing three structural protein genes VP1, VP2 and VP3 of the AAAV and recombinant baculovirus rBacGFP for expressing green fluorescence protein reporter genes; infecting insect cells Sf9 with the rBacRep, the rBacVP and the rBacGFP simultaneously, and obtaining rAAAV-GFP. According to fluorescent quantitative polymerase chain reaction (PCR) detection, each Sf9 cell can generate about 28000 VG of rAAAV-GFP, and the yield is improved by about 20 times compared with a conventional three-plasmid method. The method not only is simple and convenient in operation procedure and greatly improves virus titer, but also is easy to produce in a large scale, and the bottleneck of using rAAAV as a novel virus gene transfer vector is overcome.
Owner:王安平 +1
Pichia pastoris for expressing rotavirus expression particles as well as preparation method and application of pichia pastoris
The invention relates to pichia pastoris for expressing rotavirus expression particles as well as a preparation method and application of the pichia pastoris, and discloses a human rotavirus structural protein expression cassette, comprising the following elements: (a) an initial signal element AOX; (b) a rotavirus structural protein gene; and (c) a termination signal element TT. The invention further discloses a yeast cell transfected by using the expression cassette, a method for preparing the rotavirus viroid particles by using the yeast cell, viroid particles prepared by using the method, and application of the viroid particles for preparing vaccine compositions for preventing or treating rotavirus infection. The invention also discloses a preparation method of the yeast cell.
Owner:SHANGHAI INST OF BIOLOGICAL PROD CO LTD
Lphavirus replicon vector-based rabies virus infectious clone, and preparation method and application thereof
ActiveCN110643632AControllable positioningEasy way to getSsRNA viruses negative-senseViral antigen ingredientsViral glycoproteinTGE VACCINE
The invention belongs to the technical field of biology, and particularly discloses an alphavirus replicon vector-based rabies virus infectious clone, and a preparation method and application thereof.The infectious clone of the strain is constructed by taking Venezuelan Equine Encephalitis Virus (VEEV) vaccine strain TC-83 replicon which lacks structural protein genes as a vector and inserting rabies virus Glycoprotein (G) genes into a deletion position of the vector, and the rescued strain serving as an attenuated live vaccine not only provides effective immune protection, but also is saferto use.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI +1
Non-structural protein gene 3ABC of foot-and-mouth disease virus and its preparation and use
The present invention relates to non-structural protein gene 3ABC of foot-and-mouth disease virus, prokaryotic expression vector constituted with the gene, recombinant expression strain Escherichia coli BL21 / pKG3ABC obtained through transforming the vector to colibacillus BL21 and screening, method of expressing non-structural protein gene 3ABC of foot-and-mouth disease virus with the expression strain and purifying the expression protein, and the ELISA discrimination and diagnosis method established with the expression protein. The recombinant expression strain Escherichia coli BL21 / pKG3ABC is preserved in CCTCC and in the preservation number of CCTCC M203068.
Owner:HUAZHONG AGRI UNIV
No.6 gene segment of rice dwarfing virus
InactiveCN1344797AEasy to understandStrong RDV resistant plantsMicrobiological testing/measurementViruses/bacteriophagesIntracellularPlant cell
The present invention relates to the biological function and determination method of non-structural protein gene S6 of rice dwarf virus (RDV). The non-structural protein Pns6 is encoded by the No.6 segment S6 of RDV. The infections cloning test of Potato Virus X intercellular motion defect strain and the complementary test of RDV encoded non-structural protein gene shows that non-structural protein encoded by RDV is intercellular motion protein of RDV. Intracellular location research of Pns6 shows that the S6 protein is located in the plasmodesmus of plant cell wall and has the typical characteristic of motion protein. The determination of the gene biological function of S6 in helpful to the further research of the plant infecting mechanism of RDV and obtaining more powerful RDV resisting plant.
Owner:PEKING UNIV
Recombination expression of HPLC12 type ice structuring protein in bacillus subtilis and preparation method
InactiveCN103013896ABiosafetyGood genetic stabilityBacteriaMicroorganism based processesBiotechnologyCloning Site
The invention discloses a bacillus subtilis recombinant strain which is obtained by introducing a HPLC12 type ice structuring protein gene into bacillus subtilis. The amino acid sequence of the HPLC12 type ice structuring protein is as shown in a sequence 2. The invention also provides a preparation method of the HPLC12 type ice structuring protein. The HPLC12 type ice structuring protein is obtained by fermenting any one of the bacillus subtilis recombinant strains in the claims 1-4. The preparation method has the beneficial effects that the bacillus subtilis has biological safety and good genetic stability and can persistently secrete expression foreign genes, a HPLC12 type ice structuring protein gene subjected to codon optimization is inserted behind a multiple-cloning site starting from a bacillus subtilis plasmid pAl12 so that the bacillus subtilis recombinant engineering strain which can stably and largely secrete the HPLC12 type ice structuring proteins can be obtained. The preparation method plays an important role in the biological preparation of antifreeze protein expression.
Owner:周建业
Exogenous recombinant expression and purification method for crystallizable mature SARS coronavirus non-structural protein 12 (sars-nsp12)
InactiveCN103184228AEnsure physical and chemical stabilityStable physicochemical stabilityBacteriaTransferasesEscherichia coliPurification methods
According to the invention, a small ubiquitin-like modifier protein gene and a nsp12 gene are connected into a pET-26b vector to realize formation of a pET-26b-S-nsp12-CHis6 expression plasmid, a ubiquitin-like protein processing enzyme is linked into a pET11a vector to realize formation of a pET-11a-Ulp expression plasmid, and the aforementioned pET-26b-S-nsp12-CHis6 and the pET11a-Ulp expression plasmid are transformed into an escherichia coli BL21(DE3)pLysS to realize expression and purification of wild type protein of the nsp12. The obtained wild type protein of the nsp12 has normal RNA polymerase biological activity that should own by the wild-type protein, and can be used for crystallization experiments after necessary purification treatment, and can form a protein crystal. A method for exogenous recombinant expression and purification of crystallizable mature SARS coronavirus non-structural protein 12 (sars-nsp12) is provided by the invention.
Owner:TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
Dog type II adenovirus live vector recombinant vaccine for displaying protective antigen of rabies virus
InactiveCN101406698AResist attackStrong immunityAntiviralsAntibody medical ingredientsDiseaseProtective antigen
The invention discloses a recombinant vaccine with canine adenovirus type-2 as a live vector for displaying a protective antigen of rabies virus, which relates to recombinant vaccines displaying the protective antigen or an antigenic epitope of the rabies virus respectively by utilizing the characteristics of different structural proteins of the canine adenovirus type-2. The protective antigen of the rabies virus or a ribonucleotide sequence expressing the antigenic epitope are inserted at a hydrophobic locus of a structural protein gene to ensure that an exogenous antigen or an antigenic epitope is subject to fusion expression with structural protein of the exogenous antigen or the antigenic epitope on the surfaces of adenoviruses. After the recombinant vaccines challenge, test animals are subject to closed and isolated rearing, feeding and disease conditions of the test animals are observed and recorded; and the results show that dogs taking any kind of recombinant vaccine by oral administration and intramuscular injection all can resist the attack of strong viruses, and the survival rate is more than 90 percent.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Medaka ovary structural protein gene promoter and applications thereof
ActiveCN102936596AInstructions are accurateQuick instructionsBacteriaMicroorganism based processesPerturbateurs endocriniensGreen fluorescent protein
The present invention discloses a medaka ovary structural protein (OSP1) gene promoter and applications thereof. According to the present invention, the promoter is linked to an upstream region of green fluorescent protein gene in PEGFP-1 plasmid, and a microinjection method is adopted to inject the fusion plasmid into the fertilized egg of the medaka to construct to obtain the transgenic medaka, wherein incidence rate and occurrence degree of medaka hermaphrodite caused by exposure under exogenous endocrine interference substances can be accurately, rapidly and sensitively indicated, and the promoter can be applicable for rapid estrogen substance screening and estrogen effect substance detection in an environment.
Owner:PEKING UNIV
Expression and purification of murine norovirus VP1 protein virus-like particles and preparation of polyclonal antibody
InactiveCN107988240AFully formedStable formationSsRNA viruses positive-senseSerum immunoglobulinsLaboratory mouseNew Zealand white rabbit
The invention discloses expression and purification of murine norovirus VP1 protein virus-like particles and preparation of a polyclonal antibody. According to the technical scheme, structural proteinVP1 genes of the murine norovirus are subjected to codon optimization for the first time, protein is expressed by utilizing a baculovirus expression system, the expressed protein forms the virus-likeparticles, and the antibody can simulate capsid protein of live viruses. For a VLPs antigen, the new Zealand white rabbit is immunized, the polyclonal antibody capable of resisting op.VP1 is prepared, the experiment proves that the polyclonal antibody can be used for Western Blot detection and IFA analysis, and a basic material is provided for detecting the MNV carrying condition of the laboratory mouse in a laboratory. Meanwhile, the VLPs antigen and the polyclonal antibody lay a foundation for further developing an ELISA detection kit.
Owner:NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
Construction and function test of double-long-hairpin RNA (Ribonucleic Acid) expression element for inhibiting HIV-1 (Human Immunodeficiency Virus-1)
InactiveCN102352360ARealize multi-target RNAiEasy to operateMicrobiological testing/measurementVector-based foreign material introductionFluorescenceHuman immunodeficiency virus 1
The invention relates to construction and function test of a double-long-hairpin RNA (Ribonucleic Acid) expression element for inhibiting HIV-1 (Human Immunodeficiency Virus-1), belonging to the technical field of gene engineering. In accordance with the design principle of siRNA and the conservative analysis of the HIV-1 sequence, an HIV-1 capsid protein gag gene (532-552), an envelope protein env gene (1428-1448), a non-structural protein tat gene (131-151) and a vpu gene (143-161) are selected and designed into a corresponding siRNA sequence; through a two-step PCR (Polymerase Chain Reaction) method, a double-long-hairpin RNA expression element (the sequence list is shown as SEQ ID No.1) is finally constructed; and through microscopic fluorescent observation and flow cell analysis quantitative test, the inhibition effect of the double-long-hairpin RNA expression element on the eukaryotic expression of an HIV gene EGFP (Enhanced Green Fluorescent Protein) fusion protein is tested. Experiments prove that the dlh RNA expression element can effectively inhibit the expression of multiple HIV genes.
Owner:NANKAI UNIV
Double-effective vaccine vector against foot-and-mouth disease virus (FMDV), methods of preparing and using the same
ActiveUS20100129402A1Induce proliferative responseEasy to produceSsRNA viruses positive-senseVectorsAntisense geneStructural Protein Gene
A double-effective vaccine vector against foot-and-mouth disease virus having a bicistronic expression vector sequence, the bicistronic expression vector sequence is an antisense gene sequence capable of conjugating with 5′ UTR of RNA of the foot-and-mouth disease virus genome and an intact sequence of VP1 structural protein gene of the foot-and-mouth disease virus. Animal experiments show that the vaccine vector provides double effects in terms of gene therapy and gene immunization for the prevention and treatment of foot-and-mouth disease in animals. Also provided are construction methods and methods of use of the vaccine vector.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Avirulent, immunogenic flavivirus chimeras
InactiveUS20090010961A1Minimize and inhibit and immunize individualStable maintenanceOrganic active ingredientsSsRNA viruses positive-senseViral diseaseAmino acid mutation
Chimeric flaviviruses that are avirulent and immunogenic are provided. The chimeric viruses are constructed to contain amino acid mutations in the nonstructural proteins of a flavivirus. Chimeric viruses containing the attenuation-mutated nonstructural genes of the virus are used as a backbone into which the structural protein genes of a second flavivirus strain are inserted. These chimeric viruses elicit pronounced immunogenicity yet lack the accompanying clinical symptoms of viral disease. The attenuated chimeric viruses are effective as immunogens or vaccines and may be combined in a pharmaceutical composition to confer simultaneous immunity against several strains of pathogenic flaviviruses.
Owner:UNITED STATES OF AMERICA +1
Inducible type promoter and application thereof
ActiveCN106636097AIncrease synthetic expressionPlant peptidesVector-based foreign material introductionSesquiterpeneStructural Protein Gene
The invention relates to an inducible type promoter, and further relates to an expression vector containing the inducible type promoter and application of the inducible type promoter in regulating the expression of a target gene. The inducible type promoter has a function of starting the expression of a downstream structural protein gene under an induction condition. A technical support is provided to utilize the inducible expression mechanism of the promoter to improve the synthesis expression amount of aquilaria sinensis sesquiterpenes, and understand the mechanism related to trauma inducible expression of an aquilaria sinensis sesquiterpenes synthase gene.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Medicament carrier for targeted medicament delivery on diseases related to macrophage and preparation method thereof
InactiveCN101690822BReduce dosageLow toxicityMicroorganism based processesImmunological disordersDiseaseCoat protein
This invention discloses a medicament carrier for targeted medicament delivery on diseases related to macrophage and a preparation method thereof, the medicament carrier is hollow virus-like particles formed by prokaryotic expression and self-assembly of rotavirus coat protein Vp6 with specific identification ability to the macrophage; the internal surface or the external surface of the medicament carrier is chemically modified with medicament molecules. The preparation method comprises the following steps: firstly, extracting genes of structural protein Vp6 of A group rotavirus; secondly, cloning the genes of the structural protein Vp6 to a prokaryotic expression carrier; thirdly, performing expression in colibacillus BL21; and finally, crosslinking the expressed protein with the medicament and performing assembly to obtain the virus-like particles. By using the specific identification ability of the virus-like particles to the macrophage, medicaments are delivered to the macrophage to realize the functions of the targeted medicament delivery and slow release. The medicament carrier of the invention can effectively reduce the dosage and toxicity of the medicaments, prevent the medicaments from being degraded by internal environment, improve treating effect, and realize mass production and reduce manufacture cost at the same time.
Owner:SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
Chimeric virus of complete structural protein of hepatitis C virus and GB virus B
ActiveCN102154227ATrue reflection of the immune responseLearn about preclinical researchViruses/bacteriophagesFermentationPrimateGenotype
The invention relates to a virus, a composition and the field of preparation or purification thereof, in particular to a chimeric virus of the complete structural protein of a hepatitis C virus and a GB virus B. The chimeric virus is formed by connecting the sequence of a non-coding region on the 5' terminal of the GB virus B, the gene sequence of the complete structural protein of the hepatitis C virus, a nonstructural protein of the GB virus B and the sequence of a non-coding region on a 3' terminal in turn, wherein the gene sequence of the complete structural protein of the hepatitis C virus is the gene sequence of the complete structural protein of 1b genotype hepatitis C virus. The chimeric virus can be used to infect marmoset effectively through the intrahepatic or intravenous injection of the chimeric virus-containing serum of a primary marmoset. The chimeric virus simulates the infection and immune state of the hepatitis C virus in bodies of primates, a platform for testing and evaluating the immunity to the complete structural protein of the hepatitis C virus is provided, and the scientific problems such as limitation on the research on immunity, prevention and control ofhepatitis C virus and vaccine evaluation due to lack of small infected models of primates are solved.
Owner:SOUTHERN MEDICAL UNIVERSITY
Screening method of cell line capable of stably expressing complete structural protein CHO-K1 of hog cholera virus
PendingCN113174407ASsRNA viruses positive-senseGenetically modified cellsClassical swine fever virus CSFVRecombinant vaccines
The invention discloses a screening method of a cell line capable of stably expressing a complete structural protein CHO-K1 of a hog cholera virus, which mainly comprises the following steps: amplifying a complete structural protein (ShmEs) gene of the hog cholera virus (a rock gate strain) by utilizing a PCR (Polymerase Chain Reaction) method, and inserting the gene into an eukaryotic expression vector pcDNA3.4 to construct a recombinant expression plasmid pcDNA3.4-ShmEs. A transient transfection method is used for transferring the recombinant plasmid into CHO-K1 cells, and antibiotic G418 is used for screening. Test results show that the ShmEs gene is expressed in CHO-K1 cells and can be stably transmitted, and antigen immunized rabbit bodies produced by the stable cell line can stimulate the rabbit bodies to generate swine fever specific antibodies, and the swine fever specific antibodies can be maintained for at least 42 days. The successful expression of the ShmEs gene and the establishment of a stable cell line of the ShmEs gene lay a foundation for the development of novel genetic recombinant vaccines for swine fever.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Porcine Delta coronavirus virus-like particles as well as preparation method and application thereof
ActiveCN111187782AIntegrityImproving immunogenicitySsRNA viruses positive-senseViral antigen ingredientsVirus-like particleShuttle plasmid
The invention provides porcine Delta coronavirus virus-like particles as well as a preparation method and application thereof. The method comprises the following steps: firstly, designing and amplifying a porcine Delta coronavirus structural protein gene, constructing a recombinant shuttle plasmid by using the structural protein gene, constructing a recombinant bacmid by using the recombinant shuttle plasmid, transfecting Sf9 cells with the recombinant bacmid to obtain a recombinant baculovirus expressing the porcine Delta coronavirus structural protein, infecting Sf9 cells with the recombinant baculovirus, and performing purification to obtain the porcine Delta coronavirus-like particles. The preparation method provided by the invention uses the no-serum cultured Sf9 cells for expressionpreparation, sucrose density solution ultracentrifugation is also used to obtain the PDCoV-VLP virus-like particles, the PDCoV-VLP virus-like particles have the advantages of integrity, good immunogenicity, and high titer and safety of an antibody generated by immunizing animals, and can be used for preventing and treating porcine Delta coronavirus virus diseases, so that the particles have good development and application prospects.
Owner:SHANGHAI JIAO TONG UNIV
Non-structural protein gene NS1 of avian influenza virus and its preparation method and use
InactiveCN1303212CHigh biosecurityNo biohazardSugar derivativesBacteriaEscherichia coliCompanion animal
The invention discloses the cDNA sequences of the non-structural protein gene NS1 of avian influenza virus, its preparation process and use, wherein gene is employed for constructing prokaryotic expression vectors, the expression vectors are transformed into bacillus coli to obtain recombinant strains (Escherichia coli BL21 / pET-28a-NS1), the strains are utilized to express the non-structural protein NS1 of the avian influenza virus, the expression proteins are used to establish a differential diagnosis method through enzyme linked immunosorbent assay for differentiate vaccinum avian influenzae inactivatum immune chicken from infected chicken. The invention also relates to a differential diagnosis reagent kit and its application.
Owner:HUAZHONG AGRI UNIV
VEEV expression vector suitable for expressing antibody and application of VEEV expression vector
PendingCN113699184AControllable positioningProperly assembledSsRNA viruses positive-senseImmunoglobulins against virusesAnimals vaccinesTGE VACCINE
The invention belongs to the technical field of animal vaccines, and particularly relates to a VEEV expression vector suitable for expressing an antibody and application of VEEV expression vector. According to the invention, a Venezuelan equine encephalitis virus vaccine strain TC-83 replicon without a structural protein gene is taken as a carrier of the mRNA, a second SG promoter is inserted, and the two SG promoters are used for respectively expressing a heavy chain and a light chain of the antibody, so that one complete antibody can be expressed by one mRNA. And the mRNA can be subjected to co-transfection packaging with helper RNAs (VEEV-C and VEEV-E without nsP4) to obtain the VRP for expressing the antibody, and the VRP can be delivered in a lung targeting manner through nasal administration and is effectively expressed, so that an effective protection effect is provided for mice.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Dog type II adenovirus live vector recombinant vaccine for displaying protective antigen of rabies virus
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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