Pichia pastoris for expressing rotavirus expression particles as well as preparation method and application of pichia pastoris

A rotavirus and virus-like particle technology, applied in the field of genetic engineering, can solve problems such as side effects, intussusception, and weak cross-protection of vaccines
CN102465137AActive Publication Date: 2012-05-23SHANGHAI INST OF BIOLOGICAL PROD CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI INST OF BIOLOGICAL PROD CO LTD
Publication Date
2012-05-23

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Abstract

The invention relates to pichia pastoris for expressing rotavirus expression particles as well as a preparation method and application of the pichia pastoris, and discloses a human rotavirus structural protein expression cassette, comprising the following elements: (a) an initial signal element AOX; (b) a rotavirus structural protein gene; and (c) a termination signal element TT. The invention further discloses a yeast cell transfected by using the expression cassette, a method for preparing the rotavirus viroid particles by using the yeast cell, viroid particles prepared by using the method, and application of the viroid particles for preparing vaccine compositions for preventing or treating rotavirus infection. The invention also discloses a preparation method of the yeast cell.
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Description

field of invention

[0001] The invention relates to the field of genetic engineering, in particular, using yeast system to express rotavirus structural protein to assemble rotavirus virus-like particles for preventing rotavirus infection. Background of the invention

[0002] Rotavirus is one of the most common pathogens of severe diarrhea in infants and young children worldwide. It mainly infects small intestinal epithelial cells, causing cell damage and diarrhea. Rotavirus is prevalent in summer, autumn and winter every year. The route of infection is the fecal-oral route. The clinical manifestations are acute gastroenteritis and osmotic diarrhea. Days, severe dehydration symptoms.

[0003] There are seven types of rotavirus, which are numbered A, B, C, D, E, F, and G in English letters. Humans are mainly infected by rotaviruses A, B and C, the most common of which is rotavirus A. All seven rotaviruses can cause disease in other animals.

[0004] Among rotavirus A specie...

Claims

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