Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multi-epitope fusion diagnosis antigen for African swine fever virus as well as preparation method and application thereof

An African swine fever virus and fusion antigen technology, applied in the biological field, can solve the problems of low specificity and low sensitivity, and achieve the effect of high sensitivity and strong specificity

Inactive Publication Date: 2018-06-12
SHIHEZI UNIVERSITY
View PDF4 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that cell-cultured whole virus antigens have high sensitivity but low specificity; single recombinant protein antigens have high specificity but low sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-epitope fusion diagnosis antigen for African swine fever virus as well as preparation method and application thereof
  • Multi-epitope fusion diagnosis antigen for African swine fever virus as well as preparation method and application thereof
  • Multi-epitope fusion diagnosis antigen for African swine fever virus as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0066] The present invention will be further described in detail below through specific examples.

[0067] 1. Prediction and analysis of dominant antigenic epitopes of important antigenic genes of ASFV.

[0068] According to the whole genome sequence of ASFV (GenBank accession number: U18466.2), using biological online software ABCPred (http: / / www.imtech.res.in / raghava / abcpred / ), BepiPred 1.0 Server (http: / / www.cbs .dtu.dk / services / BepiPred / ), Immunomedicine Group (http: / / imed.med.ucm.es / Tools / antigenic.pl) and DNAStar-Protean software on ASFV important antigen genes (P72, P54, P30 and P12 ) to predict and analyze the epitope of the amino acid sequence encoded by ), and find the sequence with common dominant linear epitope by combining four different prediction methods (Table 1).

[0069] 2. Construction of ASFV multi-epitope fusion antigen gene.

[0070] Through the nucleotide sequence (GGCCCGGGC) encoding flexible amino acid (GPG), the genes encoding dominant antigenic epi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a multi-epitope fusion diagnosis antigen for African swine fever virus as well as a preparation method and application thereof. An ASFV (African swine fever virus) important structural protein gene encoding amino acid sequence is analyzed, screened and recombined through bioinformatics software, a multi-epitope fusion antigen gene is built and synthesized and is expressed in bacillus coli; through screening, the recombinant multi-epitope fusion antigen ASFV-meAg6 is obtained, so that diagnosis antigen protein with strong specificity and high sensitivity is provided foran ASFV serological diagnosis method.

Description

technical field [0001] The invention relates to an African swine fever virus (ASFV) multi-epitope fusion diagnostic antigen ASFV-meAg6, a preparation method and application thereof, and belongs to the field of biotechnology. Background technique [0002] African swine fever (ASF) is an acute, febrile, highly contagious infectious disease of pigs caused by African swine fever virus (ASFV). The virus can infect pigs of all breeds and ages. Clinically, sick pigs are characterized by high fever, loss of appetite, skin and internal organ bleeding, and the mortality rate is as high as 100%. [0003] The disease is listed as a first-class animal disease in my country, and belongs to the legally notifiable animal disease of the World Organization for Animal Health (OIE). ASF was initially prevalent mainly in Africa and eastern Europe, and was later found outside Africa in countries such as Sardinia, Spain, Portugal, the Caribbean, Brazil, and Eastern Europe. In 2007, the disease c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70G01N33/569
CPCC07K14/005C07K2319/40C12N15/62C12N15/70C12N2710/12022C12N2800/22G01N33/56983G01N2469/20
Inventor 乔军孟庆玲郭晶李杰乔梦凡张星星李重阳孟丹伍晔晖王熙凤贡莎莎蔡扩军
Owner SHIHEZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products