Chicken infectivity bronchitis virus attenuated vaccine strain and application thereof

A technology for attenuated vaccine and bronchitis, which is applied in the field of biomedicine to achieve good immune protection efficacy and good safety.

Active Publication Date: 2009-08-26
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome the deficiencies of existing IB vaccines, screen out attenuated vaccine strains with a good immune protection rate for the current epidemic strains of chicken infectious bronchitis in my...

Method used

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  • Chicken infectivity bronchitis virus attenuated vaccine strain and application thereof
  • Chicken infectivity bronchitis virus attenuated vaccine strain and application thereof
  • Chicken infectivity bronchitis virus attenuated vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 Preparation of chicken infectious bronchitis virus attenuated vaccine strain (IBV) of the present invention

[0033] The virulent IBV strain tl / CH / LDT3 / 03 was isolated, identified and preserved by the Avian Infectious Diseases Laboratory of Harbin Veterinary Research Institute in 2003. Use the tl / CH / LDT3 / 03 strain of infectious bronchitis virus to attenuate, use 9-10 day-old SPF chicken embryos to carry out continuous passage to attenuate the tl / CH / LDT3 / 03 strain, and inoculate each passage through the allantoic cavity Three chicken embryos were continuously passed to 120 generations, and the attenuation test was carried out. The P3, P10, P25, P50, P80, P100, P110 and P120 generation strains were inoculated into 15-day-old chickens respectively, and the chicks inoculated with P110 and P120 did not find morbidity and death, indicating that the strains were infected in the P100 and P120 generation. It has been completely weakened and has be...

experiment example 2

[0034] Experimental Example 2 The cultivation of attenuated vaccine strain chicken infectious bronchitis virus LDT3-A strain of the present invention

[0035] 1 Materials and methods

[0036] 1.1 Passage of virus strain tl / CH / LDT3 / 03 attenuated

[0037] The tl / CH / LDT3 / 03 strain was attenuated by continuous passage using 9-10 day-old SPF chicken embryos. Inoculate 3 chicken embryos through the allantoic cavity for each generation of virus, inoculate 100 μL per embryo, incubate at 37°C for 72 hours (discard dead embryos within 24 hours), observe the chicken embryos twice a day, if the chicken embryos are within 24-72 hours If it dies, store it at 4°C. After 72 hours, all the chicken embryos were taken out and placed at 4°C for 4-24 hours, the lesions of the chicken embryos were observed and recorded, and the allantoic fluid of the chicken embryos was aseptically collected for the next passage. Chicken embryos that survived 72 hours but had typical lesions and clea...

experiment example 3

[0086] Experimental Example 3 Attenuated Vaccine Strain Immunity Efficacy Experiment of the present invention

[0087] 1 material

[0088] Experimental vaccine: the vaccine is live chicken infectious bronchitis vaccine (LDT3-A strain) developed by Harbin Veterinary Research Institute, batch number: 2006001, 2006002, 2006003, 2006004, 2006005. 2000 feathers / bottle.

[0089] Vaccine storage conditions and validity period: -20 ℃ storage, the validity period is 12 months.

[0090] Usage and dosage Mark the feather portion according to the label of the bottle, dilute it with normal saline, draw the vaccine with a dropper, and drop one drop (about 0.03-0.05ml) for each chicken.

[0091] 2 methods

[0092] 2.1 Active immunization test

[0093] 1 to 5 groups of 3-day-old SPF chicks were inoculated with 5 batches of vaccines, and each chick was inoculated with 1 dose (about 0.03-0.05ml) by intranasal drip. The 3-day-old SPF chickens in the sixth group received one drop of sterili...

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Abstract

The invention discloses a infectivity bronchitis attenuated vaccine strain LDT3-A strain, and discloses application and application effect thereof in preventing and curing chicken infectivity bronchitis. The microorganism accession number of the attenuated vaccine strain is CGMCC-2902. The attenuated vaccine strain of the present invention has good safety and good immunization protection effect to the chicken infectivity bronchitis. The attenuated strain can be prepared into single vaccine or combined vaccine for preventing or curing infectivity bronchitis virus.

Description

technical field [0001] The invention relates to attenuated chicken infectious bronchitis virus vaccine strain and application thereof, belonging to the field of biomedicine. Background technique [0002] Avian Infectious Bronchitis (IB) is an acute, highly contagious, viral respiratory disease caused by Infectious Bronchitis Virus (IBV). Its characteristics are: coughing, sneezing, tracheal rales in sick chickens; enlarged and pale kidneys, dilated ureters, a large amount of urate deposition, and a typical "mottled kidney" appearance. [0003] Chicken infectious bronchitis was first discovered in North Dakota, USA in 1930 and first reported by Schalk and Hawn in 1931. Early studies have shown that infectious bronchitis virus mainly affects the respiratory tract of chicks (respiratory IB), and also harms rearing chickens and laying hens, reducing their resistance, egg production and egg quality. In 1962, Winterfield and Hitchner reported the emergence of nephropathic IB in ...

Claims

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Application Information

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IPC IPC(8): C12N7/08C07K14/165C12N15/50A61K39/215A61P31/14C12R1/93
Inventor 刘胜旺孔宪刚韩宗玺邵呈昊李慧昕
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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