10 results about "Site specific mutation" patented technology

The disclosed embodiments concern methods for determining sequences of interest using targeted unique molecular index (TUMI) sequences that are uniquely associated with individual polynucleotide fragments in plants, such as those present in a transgenic event, a site-specific mutation or a wild type variant. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

The present disclosure relates to antibodies and antibody conjugates having one or more site-specific mutations in the CH2 domain of the heavy chain. The antibody variants disclosed herein can have improved characteristics (e.g., thermal stability, antibody yields, antibody titers, cell-killing) relative to a parent or wild type antibody, including aglycosylated parent or wild type antibodies. Pharmaceutical compositions, diagnostic compositions and kits comprising the same, as well as methods of using these compositions and kits for therapeutic and diagnostic purposes, are also described.

The present disclosure provides methods of introducing site-specific mutations in a target cell and methods of determining efficacy of enzymes capable of introducing site-specific mutations. The present disclosure also provides methods of providing a bi-allelic sequence integration, methods of integrating of a sequence of interest into a locus in a genome of a cell, and methods of introducing a stable episomal vector in a cell. The present disclosure further provides methods of generating a human cell that is resistant to diphtheria toxin.

The invention relates to norcoclaurine synthases and substrate binding sites having one or more site-specific mutation which increase the activity, when compared to the wild type synthase, of the condensation of 4-HPAA and dopamine to (S)-norcoclaurine and / or 3,4-DhPAA and dopamine to (S)-norlaudanosoline. The inventors both identified specific mutations corresponding to at position 73, 75, 77, 82, 99, 114, 141, 142, 147, 152, 174 and / or 178 in the count according to SEQ ID No: 1, and sites corresponding to the binding domains defined in SEQ IN NO: 4 and 5, where the mutated increase of the activity may be positioned within these norcoclaurine synthases. These domains are conserved regions.

According to the application of the reagent for detecting the phosphorylation level of the S202 and T205 sites of the MAPT protein in preparation of the renal fibrosis diagnosis product, the function research of the MAPT protein is expanded from the traditional field of glomerular podocytes to renal tubular epithelial cells and the fibrosis pathological process of the renal tubular epithelial cells for the first time, and a brand new direction of the MAPT protein in renal disease research is opened up. Different from the conventional general research means which only depends on overall gene knockout or overexpression, the method provided by the invention realizes accurate analysis of the function of the MAPT key phosphorylation site by using the site-specific mutant. Through a systematic function determination experiment, the invention discloses an anti-intuition biological rule with great theoretical significance: although phosphorylation of the sites S202 and T205 obviously drives the fibrosis process of the renal tubular epithelial cells, the fibrosis phenotype cannot be improved by simulating the dephosphorylation state (S202A / T205A mutant) of the sites.

The application belongs to the field of genetic engineering and enzyme engineering, and discloses a kind of N-acetylneuraminic acid synthase mutant and its application in N-acetylneuraminic acid production, the mutant NeuB C86T is obtained by site-specific mutation on wild-type N-acetylneuraminic acid synthase from Neisseria meningitidis (N. meningitidis) Neisseria meningitidis The 86th amino acid is replaced from cysteine (C) to threonine (T). Through high performance liquid chromatography (HPLC) analysis of N-acetylneuraminic acid yield, the results show that, compared with wild-type enzyme, the mutant NeuB C86T The synthesis efficiency of N-acetylneuraminic acid is significantly improved by 57%, which indicates that it has potential superiority in producing N-acetylneuraminic acid.

The invention provides a method for increasing the drought resistance of soybean plants by introducing site-specific mutations into stomatal opening genes. The invention also relates to a soybean plant, an isolated part thereof or the seeds thereof, wherein said genes have been inactivated.

The present invention relates to compositions and methods for improved gene editing. The present disclosure provides methods of introducing a site-specific mutation in a target cell and methods of determining the efficacy of an enzyme capable of introducing a site-specific mutation. The disclosure also provides methods of providing diallele sequence integration, methods of integrating a sequence of interest into a locus in the genome of a cell, and methods of introducing a stable episomal vector in a cell. The disclosure further provides methods of producing human cells that are resistant to diphtheria toxins.

The application discloses application of a reagent for detecting phosphorylation levels of MAPT protein S202 and T205 sites in preparation of a kidney fibrosis diagnosis product, and first extends function research of the MAPT protein from a traditional glomerular podocyte field to a renal tubular epithelial cell and a fibrosis pathological process thereof, and opens up a brand-new direction of the MAPT protein in kidney disease research. Unlike previous general research means which only depend on whole gene knockout or overexpression, the application realizes accurate analysis of functions of key phosphorylation sites of the MAPT protein by using site-specific mutants. Through systematic function determination experiments, the application discloses a counterintuitive biological law with great theoretical significance: although S202 and T205 site phosphorylation significantly drives a fibrosis process of the renal tubular epithelial cell, simulation of a dephosphorylation state (S202A / T205A mutant) cannot improve the fibrosis phenotype.

The present invention belongs to the field of genetic engineering breeding technology and specifically discloses the application of targeted editing of the rice OsPLATZ1 gene to improve rice grain shape and yield. Using CRISPR / Cas genome editing technology, site-specific mutations are performed on the promoter and 5' UTR regions of the rice OsPLATZ1 gene, creating a mutant line with altered expression levels of the OsPLATZ1 gene. This alters the relative expression of the OsPLATZ1 gene, which is associated with panicle development, in the mutant line. Compared to the wild-type indica rice variety Huaguang, this new rice line was quickly and efficiently obtained with altered grain length, aspect ratio, number of grains per panicle, and 1000-grain weight. This approach satisfies people's preference for rice grain shape while simultaneously overcoming the mutual constraints between grain shape and grain number, resulting in superior lines with significantly increased yield. This approach, applied in breeding, addresses the yield challenge and provides important germplasm resources for high-yield rice breeding.