Method for breeding long-growth period japonica rice varieties by editing rice Ehd1 gene

Abstract

The invention provides a method for breeding rice varieties by transferring japonica rice in the north to the south. The method comprises the following steps: designing a CRISPR / Cas9 gene editing sitein a starting region of a rice Ehd1 gene receiving structural domain, constructing a gene editing vector, and transforming rice to achieve site-specific mutation of the Ehd1 gene so as to obtain a frameshift mutant of the Ehd1 gene and an in-frame deletion mutation strain with 3 amino acids deleted in the starting region of the receiving structural domain. Experiments prove that compared with thewild type, the growth period of the in-frame deletion mutation strain is moderately prolonged by about two weeks and the growth period of the frameshift mutant is delayed by nearly one month. By themethod provided by the invention, japonica rice materials with different prolonged growth periods can be quickly obtained, and the ecological adaptability of the existing excellent varieties is expanded; a high-efficiency gene knockout method and a high-efficiency breeding method can be provided for breeding the japonica rice varieties adapted to different ecological conditions in low-latitude regions.

Description

technical field [0001] The invention relates to the field of plant transgenic technology and crop genetic breeding, and relates to a method of editing rice using CRISPR-Cas9 technology Ehd1 The gene receiving structure domain prolongs the growth period of rice, and the breeding method of cultivating "Northern Japonica southward migration" rice varieties. Background technique [0002] Rice is the main food crop and the staple food of nearly half of the world's population. Rice is divided into two subspecies, indica and japonica. Among them, japonica rice is of high quality and its market price is generally higher than that of indica rice. Japonica rice is very popular in many countries. In order to meet the market consumption demand, the Chinese government has put forward the "indica rice to japonica rice" project focusing on improving the quality of rice. [0003] The main limiting factor affecting the introduction of northern japonica rice varieties to low-latitude areas i...

AI Technical Summary

Problems solved by technology

At present, conventional hybrid breeding is the main rice breeding method, and traditional techniques can be used to hybridize and backcross ehd1 Mutant genes are transferred into superior rice varieties to breed japonica rice varieties suitable for growth and growth in low-latitude regions. However, the main disadvantages of these breeding methods are labor-intensive, high-cost and long-breeding cycles.

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