Identification of silkworm hemocyte specific expressed gene cathepsin O regulation element

A technology for regulating elements and expressing genes, which is applied in genetic engineering, plant gene improvement, recombinant DNA technology, etc., and can solve problems such as lack of silkworm blood cells

Pending Publication Date: 2015-09-09
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect that this patented technology solves lies within itself by improving BACV production efficiency without requiring specialized equipment or genetic manipulation techniques from other organisms like humans. This can be useful both experimentally (making new drugs) and therapeutic purposes such as regulating immune responses through modifying their own proteins).

Problems solved by technology

The technical problem addressed in this patented paper relates to improving the efficiency of introducing DNA sequences called BACLAS into animal systems such as mice without causing harmful side effects like reproductively damaging them during reproduction processes.

Method used

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  • Identification of silkworm hemocyte specific expressed gene cathepsin O regulation element
  • Identification of silkworm hemocyte specific expressed gene cathepsin O regulation element
  • Identification of silkworm hemocyte specific expressed gene cathepsin O regulation element

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Experimental program
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Embodiment Construction

[0036] Material Reagent

[0037] The materials used in this experiment were Dazao (P50) and the cell line Bm-swu3, which were provided and preserved by the Silkworm Resource Bank of the State Key Laboratory of Silkworm Genome Biology, Southwest University.

[0038]

[0039] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0040] 1. Chip screening of genes specifically expressed in blood cells

[0041] The silkworm tissue microarray data (http: / / silkworm.swu.edu.cn / microarray / ) is within the scope of the silkworm group, analyzing the known and predicted expression of silkworm genes in each tissue at the 5th instar and 3 days...

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Abstract

The invention relates to a screening method of silkworm hemocyte specific expressed genes and a method for analyzing and finding a hemocyte specific expression regulation element through cloning of a protease O gene promoter. The hemocyte specific expression regulation element is manufactured through the following steps that 1, a hemocyte specific expression candidate gene protease O gene is screened out through silkworm tissue chip expression data, transcriptome data, a quantitive RT-PCR and other methods, and the silkworm hemocyte specific expression of the gene is verified successfully; 2, a related gene promoter is successfully cloned, and functional analysis is carried out; 3, a Bac-to-Bac rhabdovirus expression system is successfully improved, an effective hemocyte promoter detection system is built, the activity of the candidate gene promoter is detected at the cell and the individual level, and tissue specificity of the promoter is verified; 4, the silkworm hemocyte specific gene protease O gene promoter is obtained through the study, and it is analyzed and found that the promoter is provided with the tissue specificity regulation element in the section between the upstream -333 and -80 of a transcriptional start site. The specificity expression of the gene on hemocyte is controlled.

Description

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Claims

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Application Information

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Owner SOUTHWEST UNIVERSITY
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