Colorectal cancer diagnosis kit
A colorectal cancer, kit technology, applied in biochemical equipment and methods, DNA/RNA fragment, microorganism determination/inspection, etc. The effect of reducing error rates and overcoming limitations
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] Example 1 Screening of methylation sites (CpG sites)
[0037] The miR-17-92 cluster was analyzed using the University of California Santa Cruz (UCSC) database, and bisulfite sequencing (BSP) primers were designed to find its CpG sites, namely methylation sites. The miR-17-92 cluster CpG sites were concentrated between the upstream 3.5kb sequence and the downstream 2kb sequence of the miR-17-92 cluster transcription initiation site. figure 1 The CpG site between the upstream 2kb sequence of the transcription start site and the downstream 2kb sequence is shown, and the present invention selects the miR-17-92 cluster transcription start site upstream 1.5kb sequence to the downstream 1.5kb sequence (SED ID NO:25 )conduct experiment.
Embodiment 2
[0038] Example 2 Primer Design
[0039] The sequencing primers were designed as bisulfite sequencing (BSP) primers. The primer sequences are listed in Table 1.
[0040] Table 1 Primer sequences for methylation sites
[0041]
Embodiment 3
[0042] Embodiment 3 sample collection
[0043] The feces, colorectal cancer tissues and paracancerous tissues of 204 colorectal cancer patients were collected. The surgical resection specimens of all cases were subjected to routine pathological examination to confirm the diagnosis of cancer. The DNA or RNA was extracted in the laboratory, and the cDNA and DNA after RNA reverse transcription were stored at -20°C; and 164 age-matched normal controls were selected as controls for stool testing during the same period. All patients were treated surgically and did not receive radiotherapy or chemotherapy before operation.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com