Preparation technology of nanometer aluminum hydroxide adjuvant

A nano-aluminum hydroxide, preparation process technology, applied in medical preparations containing active ingredients, antibody medical ingredients, allergic diseases, etc. problem, to achieve the effect of good biocompatibility, good dispersibility and good dispersibility

Inactive Publication Date: 2011-03-30
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although common aluminum hydroxide adjuvant has good safety, it cannot be used as an adjuvant for tumor immunity because it cannot induce cellular immune response
However, some substances with strong adjuvant effects, such as lipopolysaccharide, cannot be used in human vaccines because of their poor safety.

Method used

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  • Preparation technology of nanometer aluminum hydroxide adjuvant
  • Preparation technology of nanometer aluminum hydroxide adjuvant
  • Preparation technology of nanometer aluminum hydroxide adjuvant

Examples

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Embodiment 1

[0018] 1. The preparation method of nano aluminum hydroxide as a cancer drug:

[0019] Choose the appropriate microemulsion formula, under 37℃ constant temperature magnetic stirring

[0020] 1. Take benzalkonium bromide and n-octanol with a mass ratio of 1:1 and shake well in a beaker;

[0021] 2. Add cyclohexane of equal mass ratio at room temperature and stir at high speed for 5 minutes, then pour it into a triangular flask and put it into the stir bar;

[0022] 3. Place the Erlenmeyer flask on the magnetic stirrer, adjust the stirring speed and add 10ml of 1mol / L AlCl 3 The solution is stirred for 10 minutes;

[0023] 4. Use a pipette to accurately measure 10ml of ammonia water in the separatory funnel to adjust the drip rate (30 drops / min as slowly as possible into the ammonia water), and maintain the system pH> 10 React for 2 hours;

[0024] 5. After the reaction, add acetone to demulsify by centrifugation (R=500rpm), discard the supernatant, then add 95% absolute ethanol and deioni...

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Abstract

The invention discloses a preparation technology of nanometer aluminum hydroxide adjuvant, comprising the following steps: taking benzalkonium bromide, n-caprylic alcohol and cyclohexane at equal ratio; stirring mixture at high speed, and pouring the mixture into a triangular flask; putting in a stir bar; putting the triangular flask on a magnetic stirrer, adding a proper quantity of AlCl3 solution, and stirring; slowing dropwise adding ammonia water into a separating funnel to regulate dripping rate; causing a reaction system to react for 2 hours at the pH of 10; after the reaction ends, adding acetone for demulsification and centrifugation; abandoning supernatant; respectively adding absolute ethyl alcohol and deionized water; evenly stirring by a glass rod; washing for three times, and decentralizing; collecting decentralized sediment into a beaker; drying for 12 hours in a drying oven to obtain fluffy Al(OH)3 adjuvant. The prepared nanometer aluminum hydroxide adjuvant has small grain diameter, good dispersibility and stability and favourable biocompatibility. In an animal experiment, the anti-tumor effect of nanometer aluminium adjuvant adsorption autovaccine is obviously superior to the curative effect obtained by using common aluminium adjuvant adsorption autovaccine.

Description

technical field [0001] The invention relates to a preparation process of a nano-aluminum adjuvant as a medicine for treating cancer and its anti-tumor therapeutic effect combined with an autologous tumor vaccine. Background technique [0002] In immunological treatment, adjuvants can enhance the body's response to vaccines in many ways: it can enhance the antigenicity of weak antigens; it can be used to enhance the duration and speed of immune responses; it can regulate the titer and specificity of antigens , isotype or differentiation; can stimulate cellular immune responses; can enhance and induce mucosal immune responses; can enhance the immunity of individuals with immature or aging immune systems; can reduce the dose or cost of antigens, and can also help overcome combination vaccines Antigen competition in . The only adjuvants currently used on a large scale are aluminum compounds, the most important of which is aluminum hydroxide. There are two main types of vaccine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P37/04
Inventor 张东生刘慧杜益群
Owner SOUTHEAST UNIV
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