Lymphocyte cultivation liquid and method and application

Abstract

The invention discloses a lymphocyte culture fluid and culture method thereof and application therefor, the culture fluid is adding ATP and coenzyme A in completely culture fluid containing basic culture fluid RPMI-1640, albumin, L-glutamine, glucose, baking soda and HEPES. The culture method includes steps: (1) preparing culture fluid; (2) suspending cell in culture fluid prepared by step (1); (3) activating cell; (4) washing activated cell by the culture fluid prepared by step (1); (5) collecting cell. The culture fluid of the invention cultures cell with higher amplification times and longer generation time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a lymphocyte culture medium and a method and application for culturing lymphocytes. Background technique [0002] Lymphocytes are a kind of immunologically active cells with important uses. By extracting lymphocytes from cancer patients and inducing culture in vitro, a large number of active cells with anti-tumor cell ability can be obtained. [0003] Cytokine-induced killer cells, referred to as CIK cells. It is a group of heterogeneous cells obtained after autologous peripheral blood mononuclear cells are co-cultured with various cytokines for a period of time in vitro. After a large amount of such cells are reinfused, it can provide tumor cell killing activity and make the body Immunity is enhanced, thereby exerting a dual effect on tumor therapy. Since CIK cells are activated autologous cells, they are safe and have no obvious side effects. Currently, CIK cells are...

AI Technical Summary

Problems solved by technology

Its energy substance is only glutamine, so there is also the problem that it cannot provide enough energy for cell culture, thus affecting cell expansion and passage